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Solution NMR structure of full-length Nsp1 from SARS-CoV-2
Authors
Wang, Y., Kirkpatrick, J.P., Carlomagno, T.
Assembly
Host translation inhibitor nsp1
Entity
1. Host translation inhibitor nsp1 (polymer), 182 monomers, 19903.18 Da Detail

GAMESLVPGF NEKTHVQLSL PVLQVRDVLV RGFGDSVEEV LSEARQHLKD GTCGLVEVEK GVLPQLEQPY VFIKRSDART APHGHVMVEL VAELEGIQYG RSGETLGVLV PHVGEIPVAY RKVLLRKNGN KGAGGHSYGA DLKSFDLGDE LGTDPYEDFQ ENWNTKHSSG VTRELMRELN GG


Formula weight
19903.18 Da
Source organism
Severe acute respiratory syndrome coronavirus 2
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 99.5 %, Completeness: 98.2 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All98.2 % (2030 of 2068)98.5 % (1052 of 1068)98.6 % (791 of 802)94.4 % (187 of 198)
Backbone98.5 % (1060 of 1076)98.7 % (374 of 379)98.7 % (516 of 523)97.7 % (170 of 174)
Sidechain98.1 % (1129 of 1151)98.4 % (678 of 689)99.1 % (434 of 438)70.8 % (17 of 24)
Aromatic93.8 % (122 of 130)93.8 % (61 of 65)93.8 % (60 of 64)100.0 % (1 of 1)
Methyl100.0 % (210 of 210)100.0 % (105 of 105)100.0 % (105 of 105)

1. entity 1

GAMESLVPGF NEKTHVQLSL PVLQVRDVLV RGFGDSVEEV LSEARQHLKD GTCGLVEVEK GVLPQLEQPY VFIKRSDART APHGHVMVEL VAELEGIQYG RSGETLGVLV PHVGEIPVAY RKVLLRKNGN KGAGGHSYGA DLKSFDLGDE LGTDPYEDFQ ENWNTKHSSG VTRELMRELN GG

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 600 uM [U-13C; U-15N] Nsp1, 50 mM sodium phosphate, 200 mM sodium chloride, 2 mM DTT, 2 mM EDTA, 0.01 % w/v sodium azide, 10 % v/v [U-2H] D2O, 90% H2O/10% D2O.


#NameIsotope labelingTypeConcentration
1Nsp1[U-13C; U-15N]600 (±100.0) uM
2sodium phosphatenatural abundance50 (±5.0) mM
3sodium chloridenatural abundance200 (±10.0) mM
4DTTnatural abundance2 (±0.1) mM
5EDTAnatural abundance2 (±0.1) mM
6sodium azidenatural abundance0.01 (±0.001) % w/v
7D2O[U-2H]10 (±0.5) % v/v
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 500 uM [U-10% 13C; U-100% 15N] Nsp1, 50 mM sodium phosphate, 200 mM sodium chloride, 2 mM DTT, 2 mM EDTA, 0.01 % w/v sodium azide, 10 % v/v [U-2H] D2O, 90% H2O/10% D2O.


#NameIsotope labelingTypeConcentration
8Nsp1[U-10% 13C; U-100% 15N]500 (±100.0) uM
9sodium phosphatenatural abundance50 (±5.0) mM
10sodium chloridenatural abundance200 (±10.0) mM
11DTTnatural abundance2 (±0.1) mM
12EDTAnatural abundance2 (±0.1) mM
13sodium azidenatural abundance0.01 (±0.001) % w/v
14D2O[U-2H]10 (±0.5) % v/v
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 6.5 (±0.1), Details 500 uM [U-10% 13C; U-100% 15N] Nsp1, 50 mM sodium phosphate, 200 mM sodium chloride, 2 mM DTT, 2 mM EDTA, 0.01 % w/w sodium azide, 10 % v/v [U-2H] D2O, 12 mg/mL Pf1 phage, 90% H2O/10% D2O.


#NameIsotope labelingTypeConcentration
15Nsp1[U-10% 13C; U-100% 15N]500 (±100.0) uM
16sodium phosphatenatural abundance50 (±5.0) mM
17sodium chloridenatural abundance200 (±10.0) mM
18DTTnatural abundance2 (±0.1) mM
19EDTAnatural abundance2 (±0.1) mM
20sodium azidenatural abundance0.01 (±0.001) % w/w
21D2O[U-2H]10 (±0.5) % v/v
22Pf1 phagenatural abundance12 (±1.0) mg/mL

Release date
2022-08-22
Citation 1
1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein)
Wang, Y., Kirkpatrick, J., zur Lage, S., Korn, S., Neissner, K., Schwalbe, H., Schlundt, A., Carlomagno, T.
Biomol. NMR Assign. (2021), 15, 287-295, PubMed 33770349 , DOI 10.1007/s12104-021-10019-6 ,
Citation 2
Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2
Wang, Y., Kirkpatrick, J.P., zur Lage, S., Carlomagno, T.
Related entities 1. Host translation inhibitor nsp1, : 1 : 13 : 9 entities Detail
Interaction partners 1. Host translation inhibitor nsp1, : 1 interactors Detail
Experiments performed 31 experiments Detail
nullKeywords Non-structural protein Host translation inhibitor, VIRAL PROTEIN