BMRB: 36009

Solution NMR structure of Humanin containing a D-isomerized serine residue

Authors

Sugiki, T., Furuita, K., Alsanousi, N., Fujiwara, T., Kojima, C.

Assembly

Humanin

Entity

1. Humanin (polymer, Thiol state: all free), 24 monomers, 2687.231 Da Detail

MAPRGFSCLL LLTXEIDLPV KRRA

Formula weight

2687.231 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 85.5 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	85.5 % (142 of 166)	84.1 % (122 of 145)	95.2 % (20 of 21)
Backbone	93.9 % (62 of 66)	93.3 % (42 of 45)	95.2 % (20 of 21)
Sidechain	80.0 % (80 of 100)	80.0 % (80 of 100)
Aromatic	80.0 % (4 of 5)	80.0 % (4 of 5)
Methyl	88.2 % (15 of 17)	88.2 % (15 of 17)

1. entity 1

MAPRGFSCLL LLTXEIDLPV KRRA

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 7, Details 2.0 mM D-Ser-containing humanin, trifluoroethanol/water

#	Name	Isotope labeling	Type	Concentration
1	D-Ser-containing humanin	natural abundance		2.0 mM
2	trifluoroethanol	U-99% D		30 %
3	H2O	natural abundance		70 %

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Calculated from 20 models in PDB: 5GIW, Strand ID: X Detail

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Release date

2016-09-07

Citation

Solution NMR structure and inhibitory effect against amyloid-β fibrillation of Humanin containing a d-isomerized serine residue
Alsanousi, N., Sugiki, T., Furuita, K., So, M., Lee, Y.H., Fujiwara, T., Kojima, C.
Biochem. Biophys. Res. Commun. (2016), 477, 647-653, PubMed 27349871 , DOI 10.1016/j.bbrc.2016.06.114 , Abstract

Related entities 1. Humanin, : 1 : 11 : 21 entities Detail

Interaction partners 1. Humanin, : 1 interactors Detail

Experiments performed 3 experiments Detail