BMRB: 36018

Solution structure of the first RRM domain of human spliceosomal protein SF3b49

Authors

Kuwasako, K., Nameki, N., Tsuda, K., Takahashi, M., Sato, A., Tochio, N., Inoue, M., Terada, T., Kigawa, T., Kobayashi, N., Shirouzu, M., Ito, T., Sakamoto, T., Wakamatsu, K., Guntert, P., Takahashi, S., Yokoyama, S., Muto, Y.

Assembly

Splicing factor 3B subunit 4

Entity

1. Splicing factor 3B subunit 4 (polymer, Thiol state: not present), 105 monomers, 11365.59 Da Detail

GSSGSSGPIS ERNQDATVYV GGLDEKVSEP LLWELFLQAG PVVNTHMPKD RVTGQHQGYG FVEFLSEEDA DYAIKIMDMI KLYGKPIRVN KASAHNKNLS GPSSG

Formula weight

11365.59 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.6 %, Completeness: 89.4 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.4 % (1059 of 1184)	89.4 % (551 of 616)	90.0 % (413 of 459)	87.2 % (95 of 109)
Backbone	86.7 % (536 of 618)	86.1 % (186 of 216)	87.1 % (264 of 303)	86.9 % (86 of 99)
Sidechain	92.3 % (608 of 659)	91.3 % (365 of 400)	93.6 % (233 of 249)	100.0 % (10 of 10)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (42 of 42)	100.0 % (1 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. Splicing factor 3B subunit 4

GSSGSSGPIS ERNQDATVYV GGLDEKVSEP LLWELFLQAG PVVNTHMPKD RVTGQHQGYG FVEFLSEEDA DYAIKIMDMI KLYGKPIRVN KASAHNKNLS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.8 mM [U-100% 13C; U-100% 15N] human spliceosomal protein SF3b49, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	human spliceosomal protein SF3b49	[U-100% 13C; U-100% 15N]	protein	0.8 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 5GVQ, Strand ID: A Detail

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Release date

2016-10-26

Citation

Solution structure of the first RNA recognition motif domain of human spliceosomal protein SF3b49 and its mode of interaction with a SF3b145 fragment
Kuwasako, K., Nameki, N., Tsuda, K., Takahashi, M., Sato, A., Tochio, N., Inoue, M., Terada, T., Kigawa, T., Kobayashi, N., Shirouzu, M., Ito, T., Sakamoto, T., Wakamatsu, K., Guntert, P., Takahashi, S., Yokoyama, S., Muto, Y.
Protein Sci. (2017), 26, 280-291, PubMed 27862552 , DOI 10.1002/pro.3080 , Abstract

Related entities 1. Splicing factor 3B subunit 4, : 1 : 137 entities Detail

Interaction partners 1. Splicing factor 3B subunit 4, : 62 interactors Detail

Experiments performed 4 experiments Detail