Solution structure of human Gelsolin protein domain 1 at pH 5.0
EHPEFLKAGK EPGLQIWRVE KFDLVPVPTN LYGDFFTGDA YVILKTVQLR NGNLQYDLHY WLGNECSQDE SGAAAIFTVQ LDDYLNGRAV QHREVQGFES ATFLGYFKSG LKYKKGGVAS GFKHVVPNEV VVQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.8 % (945 of 1579) | 76.0 % (620 of 816) | 31.7 % (196 of 619) | 89.6 % (129 of 144) |
Backbone | 60.4 % (476 of 788) | 96.7 % (266 of 275) | 21.8 % (84 of 385) | 98.4 % (126 of 128) |
Sidechain | 54.7 % (498 of 910) | 65.4 % (354 of 541) | 39.9 % (141 of 353) | 18.8 % (3 of 16) |
Aromatic | 64.0 % (119 of 186) | 64.5 % (60 of 93) | 63.7 % (58 of 91) | 50.0 % (1 of 2) |
Methyl | 85.6 % (125 of 146) | 93.2 % (68 of 73) | 78.1 % (57 of 73) |
1. entity 1
EHPEFLKAGK EPGLQIWRVE KFDLVPVPTN LYGDFFTGDA YVILKTVQLR NGNLQYDLHY WLGNECSQDE SGAAAIFTVQ LDDYLNGRAV QHREVQGFES ATFLGYFKSG LKYKKGGVAS GFKHVVPNEV VVQSolvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1), Details 0.65 mM 13C, 15N gelsolin domain 1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | gelsolin domain 1 | [U-13C; U-15N] | protein | 0.65 (±0.1) mM |
2 | Acetate | natural abundance | buffer | 10 mM |
3 | H2O | natural abundance | solvent | 90 % |
4 | D2O | [U-2H] | solvent | 10 % |