BMRBj - BMREntryMaster

Found 1 Document (0.638 seconds)

BMRB: 36078

5XIR

Solution structure for human HSP70 substrate binding domain L542Y mutant

Authors

Hoshikawa, M., Tochio, N., Tate, S.

Assembly

Heat shock 70 kDa protein 1A

Entity

1. Heat shock 70 kDa protein 1A (polymer, Thiol state: not present), 185 monomers, 20349.68 Da Detail

HMGDKSENVQ DLLLLDVAPL SLGLETAGGV MTALIKRNST IPTKQTQIFT TYSDNQPGVL IQVYEGERAM TKDNNLLGRF ELSGIPPAPR GVPQIEVTFD IDANGILNVT ATDKSTGKAN KITITNDKGR LSKEEIERMV QEAEKYKAED EVQRERVSAK NAYESYAFNM KSAVEDEGLK GKISE

Formula weight

20349.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 88.0 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.0 % (1860 of 2113)	81.8 % (902 of 1103)	94.6 % (769 of 813)	95.9 % (189 of 197)
Backbone	95.8 % (1050 of 1096)	93.6 % (353 of 377)	96.9 % (524 of 541)	97.2 % (173 of 178)
Sidechain	82.2 % (976 of 1188)	75.6 % (549 of 726)	92.8 % (411 of 443)	84.2 % (16 of 19)
Aromatic	95.2 % (80 of 84)	95.2 % (40 of 42)	95.2 % (40 of 42)
Methyl	92.0 % (195 of 212)	90.6 % (96 of 106)	93.4 % (99 of 106)

1. Heat shock 70 kDa protein 1A

Show sample condition

Sample

Solvent system 94% H2O/6% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0, Details 50 mM potassium phosphate, 50 mM potassium chloride, 10 mM DTT, 94% H2O/6% D2O

#	Name	Isotope labeling	Type	Concentration
1	human HSP70 substrate binding domain L542Y mutant		protein	0.0 ~ 0.0 mM
2	DTT	natural abundance		10 mM
3	potassium chloride	natural abundance		50 mM
4	potassium phosphate	natural abundance		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Release date

2017-07-05

Citation

Substrate Binding Switches the Conformation at the Lynchpin Site in the Substrate-Binding Domain of Human Hsp70 to Enable Allosteric Interdomain Communication
Umehara, K., Hoshikawa, M., Tochio, N., Tate, S.I.
Molecules (2018), 23, E528-E528, PubMed 29495458 , DOI 10.3390/molecules23030528 , Abstract

Related entities 1. Heat shock 70 kDa protein 1A, : 1 : 7 : 285 entities Detail

Interaction partners 1. Heat shock 70 kDa protein 1A, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 3D HNCO