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BMRB: 36085

5XN4

Anti-CRISPR protein AcrIIA4

Authors

Suh, J.-Y, Kim, I.

Assembly

Anti-CRISPR AcrIIA4

Entity

1. Anti-CRISPR AcrIIA4 (polymer, Thiol state: not present), 87 monomers, 10174.01 Da Detail

MNINDLIREI KNKDYTVKLS GTDSNSITQL IIRVNNDGNE YVISESENES IVEKFISAFK NGWNQEYEDE EEFYNDMQTI TLKSELN

Formula weight

10174.01 Da

Source organism

Listeria monocytogenes

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.5 % (960 of 1038)	92.3 % (500 of 542)	91.9 % (361 of 393)	96.1 % (99 of 103)
Backbone	97.3 % (508 of 522)	96.0 % (170 of 177)	98.8 % (255 of 258)	95.4 % (83 of 87)
Sidechain	89.2 % (535 of 600)	90.4 % (330 of 365)	86.3 % (189 of 219)	100.0 % (16 of 16)
Aromatic	52.7 % (39 of 74)	54.1 % (20 of 37)	50.0 % (18 of 36)	100.0 % (1 of 1)
Methyl	92.0 % (81 of 88)	97.7 % (43 of 44)	86.4 % (38 of 44)

1. Anti-CRISPR AcrIIA4

MNINDLIREI KNKDYTVKLS GTDSNSITQL IIRVNNDGNE YVISESENES IVEKFISAFK NGWNQEYEDE EEFYNDMQTI TLKSELN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 2.0 mM [U-99% 13C; U-99% 15N] AcrIIA4, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	AcrIIA4	[U-99% 13C; U-99% 15N]	protein	2.0 mM
2	Tris HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.93 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.93 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Release date

2018-04-22

Citation

Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor
Kim, I., Jeong, M., Ka, D., Han, M., Kim, N.K., Bae, E., Suh, J.Y.
Sci. Rep. (2018), 8, 3883-3883, PubMed 29497118 , DOI 10.1038/s41598-018-22177-0 , Abstract

Related entities 1. Anti-CRISPR AcrIIA4, : 1 : 3 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC