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BMRB: 36101

5XV8

Solution structure of the complex between UVSSA acidic region and TFIIH p62 PH domain

Authors

Okuda, M., Nishimura, Y.

Assembly

DP1, General transcription factor IIH subunit 1

Entity

1. DP1, General transcription factor IIH subunit 1, entity 1 (polymer, Thiol state: not present), 48 monomers, 5500.752 Da Detail

GSMRRRTEAL GDAEEDEDDE DFVEVPEKEG YEPHIPDHLR PEYGLEAA

2. DP1, General transcription factor IIH subunit 1, entity 2 (polymer, Thiol state: all free), 110 monomers, 12428.26 Da Detail

GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN

Total weight

17929.012 Da

Max. entity weight

12428.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 44.3 %, Completeness: 34.6 %, Completeness (bb): 38.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	34.6 % (640 of 1852)	34.8 % (339 of 974)	33.8 % (242 of 716)	36.4 % (59 of 162)
Backbone	38.8 % (362 of 934)	39.0 % (124 of 318)	38.7 % (180 of 465)	38.4 % (58 of 151)
Sidechain	31.3 % (334 of 1067)	32.6 % (214 of 656)	30.0 % (120 of 400)	0.0 % (0 of 11)
Aromatic	22.8 % (26 of 114)	26.3 % (15 of 57)	19.6 % (11 of 56)	0.0 % (0 of 1)
Methyl	22.2 % (35 of 158)	22.8 % (18 of 79)	21.5 % (17 of 79)

1. entity 1

GSMRRRTEAL GDAEEDEDDE DFVEVPEKEG YEPHIPDHLR PEYGLEAA

2. entity 2

GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	TFIIH p62	natural abundance	protein	0.48 mM
6	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
7	D2O	[U-2H]	solvent	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
13	UVSSA	natural abundance	protein	0.48 mM
14	D2O	[U-2H]	solvent	100 %

Chem. Shift Complete2

Sequence coverage: 71.5 %, Completeness: 51.6 %, Completeness (bb): 54.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.6 % (1910 of 3704)	51.2 % (997 of 1948)	51.5 % (737 of 1432)	54.3 % (176 of 324)
Backbone	54.2 % (1012 of 1868)	54.7 % (348 of 636)	53.8 % (500 of 930)	54.3 % (164 of 302)
Sidechain	49.6 % (1058 of 2134)	49.4 % (648 of 1312)	49.9 % (399 of 800)	50.0 % (11 of 22)
Aromatic	36.8 % (84 of 228)	39.5 % (45 of 114)	33.9 % (38 of 112)	50.0 % (1 of 2)
Methyl	51.6 % (163 of 316)	51.9 % (82 of 158)	51.3 % (81 of 158)

1. entity 1

GSMRRRTEAL GDAEEDEDDE DFVEVPEKEG YEPHIPDHLR PEYGLEAA

2. entity 2

GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	TFIIH p62	natural abundance	protein	0.48 mM
6	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
7	D2O	[U-2H]	solvent	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
13	UVSSA	natural abundance	protein	0.48 mM
14	D2O	[U-2H]	solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 3 Detail

Release date

2017-12-26

Citation

Common TFIIH recruitment mechanism in global genome and transcription-coupled repair subpathways
Okuda, M., Nakazawa, Y., Guo, C., Ogi, T., Nishimura, Y.
Nucleic Acids Res. (2017), 45, 13043-13055, PubMed 29069470 , DOI 10.1093/nar/gkx970 , Abstract

Related entities 1. DP1, General transcription factor IIH subunit 1, entity 1, : 1 : 2 : 5 entities Detail

Related entities 2. DP1, General transcription factor IIH subunit 1, entity 2, : 5 : 6 entities Detail

Interaction partners 1. DP1, General transcription factor IIH subunit 1, entity 1, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Interaction partners 2. DP1, General transcription factor IIH subunit 1, entity 2, : 15 interactors Detail

More details for 15 interactors identified by 12 citations

Experiments performed 23 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

2 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

3 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

4 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

5 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

6 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

7 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

8 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	TFIIH p62	natural abundance	protein	0.48 mM
6	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
7	D2O	[U-2H]	solvent	100 %

9 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	TFIIH p62	natural abundance	protein	0.48 mM
6	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
7	D2O	[U-2H]	solvent	100 %

10 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	TFIIH p62	natural abundance	protein	0.48 mM
6	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
7	D2O	[U-2H]	solvent	100 %

11 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

12 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
13	UVSSA	natural abundance	protein	0.48 mM
14	D2O	[U-2H]	solvent	100 %

13 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
13	UVSSA	natural abundance	protein	0.48 mM
14	D2O	[U-2H]	solvent	100 %

14 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
13	UVSSA	natural abundance	protein	0.48 mM
14	D2O	[U-2H]	solvent	100 %

15 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

16 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

17 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

18 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

19 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

20 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

21 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

22 3D HNHB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] TFIIH p62, 0.48 mM UVSSA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	TFIIH p62	[U-99% 13C; U-99% 15N]	protein	0.40 mM
9	UVSSA	natural abundance	protein	0.48 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

23 3D HNHB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.80, Details 0.40 mM [U-99% 13C; U-99% 15N] UVSSA, 0.48 mM TFIIH p62, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIH p62	natural abundance	protein	0.48 mM
2	UVSSA	[U-99% 13C; U-99% 15N]	protein	0.40 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

nullKeywords DNA repair factor, NUCLEAR PROTEIN, general transcription factor, nucleotide excision repair, transcription-coupled repair

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Found 1 Document (1.265 seconds)

BMRB: 36101

Sample #1

Sample #2

Sample #3

Sample #4

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. DP1, General transcription factor IIH subunit 1, entity 1, : 1 : 2 : 5 entities Detail

Related entities 2. DP1, General transcription factor IIH subunit 1, entity 2, : 5 : 6 entities Detail

Interaction partners 1. DP1, General transcription factor IIH subunit 1, entity 1, : 3 interactors Detail

Interaction partners 2. DP1, General transcription factor IIH subunit 1, entity 2, : 15 interactors Detail

Experiments performed 23 experiments Detail

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Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample