NMR-Based Model of the 22 Amino Acid Peptide in Polysialyltransferase Domain (PSTD) of the Polysialyltransferase ST8Sia IV in the Presence of Polysialic Acid (PolySia)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.7 % (235 of 284) | 90.0 % (135 of 150) | 71.4 % (80 of 112) | 90.9 % (20 of 22) |
Backbone | 81.3 % (104 of 128) | 95.3 % (41 of 43) | 67.7 % (44 of 65) | 95.0 % (19 of 20) |
Sidechain | 84.7 % (150 of 177) | 87.9 % (94 of 107) | 80.9 % (55 of 68) | 50.0 % (1 of 2) |
Aromatic | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (11 of 11) | 100.0 % (1 of 1) |
Methyl | 71.9 % (23 of 32) | 93.8 % (15 of 16) | 50.0 % (8 of 16) |
1. entity 1
LRLIHAVRGY WLTNKVPIKR PSSolvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 1 mM PSTD-22AA-PolySia, 0.25 mM PolySia, 40 v/v ethyl group deuterated trifluoroethanol-d5, trifluoroethanol/water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PSTD-22AA-PolySia | natural abundance | protein | 1 mM |
2 | PolySia | natural abundance | 0.25 mM | |
3 | trifluoroethanol-d3 | [U-2H] | solvent | 40 v/v |
4 | H2O | natural abundance | solvent | 60 v/v |