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NMR structure of YAP1-2 WW1 domain with LATS1 PPxY motif complex
Authors
Fan, J.S., Sivaraman, J.
Assembly
WW domain with PPxY motif
Entity
1. WW domain with PPxY motif (polymer, Thiol state: not present), 59 monomers, 6571.195 Da Detail

SFEIPDDVPL PAGWEMAKTS SGQRYFLNHI DQTTTWQDPR KAMGGGGNYQ GPPPPYPKH


Formula weight
6571.195 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.3 %, Completeness: 81.3 %, Completeness (bb): 75.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All81.3 % (557 of 685)88.7 % (321 of 362)67.9 % (180 of 265)96.6 % (56 of 58)
Backbone75.0 % (252 of 336)89.7 % (104 of 116)58.8 % (100 of 170)96.0 % (48 of 50)
Sidechain88.3 % (354 of 401)88.2 % (217 of 246)87.8 % (129 of 147)100.0 % (8 of 8)
Aromatic63.2 % (48 of 76)65.8 % (25 of 38)58.3 % (21 of 36)100.0 % (2 of 2)
Methyl100.0 % (34 of 34)100.0 % (17 of 17)100.0 % (17 of 17)

1. entity 1

SFEIPDDVPL PAGWEMAKTS SGQRYFLNHI DQTTTWQDPR KAMGGGGNYQ GPPPPYPKH

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1.0 mM [U-100% 13C; U-100% 15N] WW domain with PPxY motif, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1WW domain with PPxY motif[U-100% 13C; U-100% 15N]protein1.0 (±0.1) mM
2H2Onatural abundancesolvent90 %
3D2O[U-2H]solvent10 %
Sample #2

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1 mM WW domain with PPxY motif, 100% D2O


#NameIsotope labelingTypeConcentration
4WW domain with PPxY motifnatural abundanceprotein1 (±0.1) mM
5D2O[U-2H]solvent100 %

LACS Plot; CA
Referencing offset: -0.05 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.05 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.19 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 5YDX, Strand ID: A Detail


Release date
2017-10-17
Citation
Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction
Verma, A., Jing-Song, F., Finch-Edmondson, M.L., Velazquez-Campoy, A., Balasegaran, S., Sudol, M., Sivaraman, J.
Oncotarget (2018), 9, 8068-8080, PubMed 29487715 , DOI 10.18632/oncotarget.23909 ,
Related entities 1. WW domain with PPxY motif, : 1 : 92 entities Detail
Experiments performed 5 experiments Detail
Chemical shift validation 3 contents Detail
Keywords Hippo pathway, LATs, PPxY motif, SIGNALING PROTEIN, WW domain, YAP