BMRB: 36119

Solution Structure of the DISC1/Ndel1 complex

Authors

Ye, F., Yu, C., Yu, C., Zhang, M.

Assembly

DISC1 Ndel1

Entity

1. DISC1 Ndel1 (polymer, Thiol state: all free), 145 monomers, 15438.27 Da Detail

GSEFGPWKED SHIVSAEVGE KCEAIGVKLL HLEDQLLGAM YSHDEALFQS LQGELQTVKE TLQAMILQLQ PTKEAGEASA SYPTAGAQET EALVPRGSGF GTSPLTPSAR ISALNIVGDL LRKVGALESK LAACRNFAKD QASRK

Formula weight

15438.27 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 84.1 %, Completeness (bb): 84.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.1 % (1357 of 1614)	90.3 % (755 of 836)	74.5 % (467 of 627)	89.4 % (135 of 151)
Backbone	84.7 % (727 of 858)	97.3 % (289 of 297)	72.0 % (304 of 422)	96.4 % (134 of 139)
Sidechain	85.8 % (762 of 888)	86.5 % (466 of 539)	87.5 % (295 of 337)	8.3 % (1 of 12)
Aromatic	0.0 % (0 of 80)	0.0 % (0 of 40)	0.0 % (0 of 39)	0.0 % (0 of 1)
Methyl	98.8 % (168 of 170)	100.0 % (85 of 85)	97.6 % (83 of 85)

1. Disrupted in schizophrenia 1 homolog,Nuclear distribution protein nudE-like 1

GSEFGPWKED SHIVSAEVGE KCEAIGVKLL HLEDQLLGAM YSHDEALFQS LQGELQTVKE TLQAMILQLQ PTKEAGEASA SYPTAGAQET EALVPRGSGF GTSPLTPSAR ISALNIVGDL LRKVGALESK LAACRNFAKD QASRK

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] DISC1_Ndel1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	DISC1_Ndel1	[U-99% 13C; U-99% 15N]	protein	0.8 mM
2	NaCl	natural abundance	salt	50 mM
3	D2O	U-2H	solvent	100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.5, Details 0.8 mM [U-99% 15N] DISC1_Ndel1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	DISC1_Ndel1	[U-99% 15N]	protein	0.8 mM
5	NaCl	natural abundance	salt	50 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] DISC1_Ndel1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	DISC1_Ndel1	[U-99% 13C; U-99% 15N]	protein	0.8 mM
9	NaCl	natural abundance	salt	50 mM
10	H2O	natural abundance	solvent	90 %
11	D2O	[U-2H]	solvent	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.5, Details 0.8 mM no labelling DISC1_Ndel1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
12	DISC1_Ndel1	natural abundance	protein	0.8 mM
13	NaCl	natural abundance	salt	50 mM
14	D2O	[U-2H]	solvent	100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 5YI4, Strand ID: A Detail

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Release date

2017-10-19

Citation

DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis
Ye, F., Kang, E., Yu, C., Qian, X., Jacob, F., Yu, C., Mao, M., Poon, R., Kim, J., Song, H., Ming, G.L., Zhang, M.
Neuron (2017), 96, 1041-1054, PubMed 29103808 , DOI 10.1016/j.neuron.2017.10.010 , Abstract

Related entities 1. DISC1 Ndel1, : 1 : 8 entities Detail

Experiments performed 5 experiments Detail