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Found 1 Document (0.756 seconds)

BMRB: 36129

5YQ3

Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter

Authors

Bhuyan, A.K., Sashi, P.

Assembly

At3g55470

Entity

1. At3g55470 (polymer, Thiol state: not present), 162 monomers, 18125.17 Da Detail

MAVGILEVSL ISGKGLKRSD FLGKIDPYVE IQYKGQTRKS SVAKEDGGRN PTWNDKLKWR AEFPGSGADY KLIVKVMDHD TFSSDDFIGE ATVHVKELLE MGVEKGTAEL RPTKYNIVDS DLSFVGELLI GVSYSLLQDR GMDGEQFGGW KHSNVDHHHH HH

Formula weight

18125.17 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.1 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.1 % (1521 of 1876)	88.5 % (861 of 973)	68.8 % (505 of 734)	91.7 % (155 of 169)
Backbone	83.2 % (802 of 964)	96.4 % (326 of 338)	68.8 % (322 of 468)	97.5 % (154 of 158)
Sidechain	80.3 % (848 of 1056)	83.8 % (532 of 635)	77.1 % (316 of 410)	0.0 % (0 of 11)
Aromatic	28.5 % (49 of 172)	46.5 % (40 of 86)	10.8 % (9 of 83)	0.0 % (0 of 3)
Methyl	93.3 % (153 of 164)	91.5 % (75 of 82)	95.1 % (78 of 82)

1. entity 1

MAVGILEVSL ISGKGLKRSD FLGKIDPYVE IQYKGQTRKS SVAKEDGGRN PTWNDKLKWR AEFPGSGADY KLIVKVMDHD TFSSDDFIGE ATVHVKELLE MGVEKGTAEL RPTKYNIVDS DLSFVGELLI GVSYSLLQDR GMDGEQFGGW KHSNVDHHHH HH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.2, Details 100 uM [U-99% 13C; U-99% 15N] Arabidopsis thaliana phloem protein 16-1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	internal	indirect	0.2514495
¹H	TSP	protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Release date

2018-04-18

Citation

Solution NMR Structure and Backbone Dynamics of Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, a Putative mRNA Transporter
Sashi, P., Singarapu, K.K., Bhuyan, A.K.
Biochemistry (2018), 57, 912-924, PubMed 29320165 , DOI 10.1021/acs.biochem.7b01071 , Abstract

Related entities 1. At3g55470, : 1 : 1 : 49 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.2, Details 100 uM [U-99% 13C; U-99% 15N] Arabidopsis thaliana phloem protein 16-1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

2 3D HNCACB

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

3 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

4 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

5 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

7 3D HNCO

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

8 3D HNCA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

9 3D HN(CO)CA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana phloem protein 16-1	[U-99% 13C; U-99% 15N]	protein	100 uM
2	Sodium Acetate	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

nullKeywords PLANT PROTEIN, STRUCTURE FROM CYANA 2.1

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Found 1 Document (0.756 seconds)

BMRB: 36129

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. At3g55470, : 1 : 1 : 49 entities Detail

Experiments performed 9 experiments Detail

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