BMRBj - BMREntryMaster

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BMRB: 36176

5ZMR

Solution Structure of the N-terminal Domain of the Yeast Rpn5

Authors

Zhang, W., Zhao, C., Li, H., Hu, Y., Jin, C.

Assembly

26S proteasome regulatory subunit RPN5

Entity

1. 26S proteasome regulatory subunit RPN5 (polymer, Thiol state: all free), 136 monomers, 15594.83 Da Detail

MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS DLASSKEVLA KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV MEYLKSSKSL DLNTRISVIE TIRVVTENKI FVEVER

Formula weight

15594.83 Da

Source organism

Saccharomyces cerevisiae S288C

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chemcal Shifts

¹H: 866, ¹³C: 557, ¹⁵N: 144 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err
1	1	2	SER	HA	H	4.265	0.02
2	1	2	SER	HB2	H	3.977	0.02
3	1	2	SER	HB3	H	3.903	0.02
4	1	2	SER	C	C	175.479	0.3
5	1	2	SER	CA	C	61.193	0.3
6	1	2	SER	CB	C	62.328	0.3
7	1	3	ARG	H	H	8.563	0.02
8	1	3	ARG	HA	H	4.751	0.02
9	1	3	ARG	HB2	H	2.03	0.02
10	1	3	ARG	C	C	176.1	0.3
11	1	3	ARG	CA	C	62.146	0.3
12	1	3	ARG	CB	C	34.247	0.3
13	1	3	ARG	N	N	119.034	0.3
14	1	4	ASP	H	H	8.398	0.02
15	1	4	ASP	HA	H	4.068	0.02
16	1	4	ASP	HB2	H	1.848	0.02
17	1	4	ASP	CA	C	61.643	0.3
18	1	4	ASP	CB	C	38.483	0.3
19	1	4	ASP	N	N	121.994	0.3
20	1	6	PRO	HA	H	4.419	0.02
21	1	6	PRO	HB2	H	2.273	0.02
22	1	6	PRO	HB3	H	1.846	0.02
23	1	6	PRO	HG2	H	2.01	0.02
24	1	6	PRO	HD2	H	3.774	0.02
25	1	6	PRO	HD3	H	3.627	0.02
26	1	6	PRO	C	C	176.671	0.3
27	1	6	PRO	CA	C	62.925	0.3
28	1	6	PRO	CB	C	32.019	0.3
29	1	6	PRO	CG	C	27.363	0.3
30	1	6	PRO	CD	C	50.485	0.3
31	1	7	ILE	H	H	8.193	0.02
32	1	7	ILE	HA	H	4.099	0.02
33	1	7	ILE	HB	H	1.808	0.02
34	1	7	ILE	HG12	H	1.496	0.02
35	1	7	ILE	HG13	H	1.189	0.02
36	1	7	ILE	HG21	H	0.901	0.02
37	1	7	ILE	HG22	H	0.901	0.02
38	1	7	ILE	HG23	H	0.901	0.02
39	1	7	ILE	HD11	H	0.867	0.02
40	1	7	ILE	HD12	H	0.867	0.02
41	1	7	ILE	HD13	H	0.867	0.02
42	1	7	ILE	C	C	176.039	0.3
43	1	7	ILE	CA	C	61.049	0.3
44	1	7	ILE	CB	C	38.815	0.3
45	1	7	ILE	CG1	C	27.379	0.3
46	1	7	ILE	CG2	C	17.323	0.03
47	1	7	ILE	CD1	C	12.893	0.3
48	1	7	ILE	N	N	121.315	0.3
49	1	8	LYS	H	H	8.359	0.02
50	1	8	LYS	HA	H	4.338	0.02
51	1	8	LYS	HB2	H	1.787	0.02
52	1	8	LYS	HB3	H	1.702	0.02
53	1	8	LYS	HG2	H	1.411	0.02
54	1	8	LYS	HG3	H	1.364	0.02
55	1	8	LYS	HD3	H	1.653	0.02
56	1	8	LYS	HE2	H	2.946	0.02
57	1	8	LYS	HZ1	H	7.492	0.02
58	1	8	LYS	HZ2	H	7.492	0.02
59	1	8	LYS	HZ3	H	7.492	0.02
60	1	8	LYS	C	C	175.565	0.3
61	1	8	LYS	CA	C	55.898	0.3
62	1	8	LYS	CB	C	33.321	0.3
63	1	8	LYS	CG	C	24.666	0.3
64	1	8	LYS	CD	C	29.16	0.3
65	1	8	LYS	CE	C	42.159	0.3
66	1	8	LYS	N	N	126.539	0.3
67	1	9	ALA	H	H	8.387	0.02
68	1	9	ALA	HA	H	4.375	0.02
69	1	9	ALA	HB1	H	1.341	0.02
70	1	9	ALA	HB2	H	1.341	0.02
71	1	9	ALA	HB3	H	1.341	0.02
72	1	9	ALA	C	C	177.588	0.3
73	1	9	ALA	CA	C	52.205	0.3
74	1	9	ALA	CB	C	19.601	0.3
75	1	9	ALA	N	N	126.572	0.3
76	1	10	ASP	H	H	8.482	0.02
77	1	10	ASP	HA	H	4.512	0.02
78	1	10	ASP	HB2	H	2.579	0.02
79	1	10	ASP	HB3	H	2.647	0.02
80	1	10	ASP	C	C	176.033	0.3
81	1	10	ASP	CA	C	54.909	0.3
82	1	10	ASP	CB	C	41.429	0.3
83	1	10	ASP	N	N	120.361	0.3
84	1	11	LYS	H	H	7.813	0.02
85	1	11	LYS	HA	H	4.197	0.02
86	1	11	LYS	HB2	H	1.63	0.02
87	1	11	LYS	HG2	H	1.259	0.02
88	1	11	LYS	HG3	H	1.074	0.02
89	1	11	LYS	HD2	H	1.549	0.02
90	1	11	LYS	HE2	H	2.822	0.02
91	1	11	LYS	HE3	H	2.769	0.02
92	1	11	LYS	C	C	174.09	0.3
93	1	11	LYS	CA	C	55.886	0.3
94	1	11	LYS	CB	C	34.426	0.3
95	1	11	LYS	CG	C	24.836	0.3
96	1	11	LYS	CD	C	29.034	0.3
97	1	11	LYS	CE	C	42.022	0.3
98	1	11	LYS	N	N	118.944	0.3
99	1	12	ASP	H	H	7.875	0.02
100	1	12	ASP	HA	H	4.704	0.02
101	1	12	ASP	HB2	H	2.731	0.02
102	1	12	ASP	HB3	H	2.617	0.02
103	1	12	ASP	C	C	177.235	0.3
104	1	12	ASP	CA	C	52.527	0.3
105	1	12	ASP	CB	C	41.782	0.3
106	1	12	ASP	N	N	119.946	0.3
107	1	13	TYR	H	H	9.37	0.02
108	1	13	TYR	HA	H	4.201	0.02
109	1	13	TYR	HB2	H	3.058	0.02
110	1	13	TYR	HB3	H	2.812	0.02
111	1	13	TYR	C	C	176.497	0.3
112	1	13	TYR	CA	C	60.542	0.3
113	1	13	TYR	CB	C	38.278	0.3
114	1	13	TYR	N	N	124.997	0.3
115	1	14	SER	H	H	8.614	0.02
116	1	14	SER	HA	H	3.857	0.02
117	1	14	SER	HB2	H	3.765	0.02
118	1	14	SER	C	C	177.105	0.3
119	1	14	SER	CA	C	62.661	0.3
120	1	14	SER	N	N	117.441	0.3
121	1	15	GLN	H	H	8.172	0.02
122	1	15	GLN	HA	H	4.023	0.02
123	1	15	GLN	HB2	H	1.999	0.02
124	1	15	GLN	HG2	H	2.316	0.02
125	1	15	GLN	HE21	H	7.663	0.02
126	1	15	GLN	HE22	H	6.797	0.02
127	1	15	GLN	C	C	178.36	0.3
128	1	15	GLN	CA	C	58.044	0.3
129	1	15	GLN	CB	C	27.996	0.3
130	1	15	GLN	CG	C	33.614	0.3
131	1	15	GLN	N	N	121.176	0.3
132	1	15	GLN	NE2	N	112.857	0.3
133	1	16	ILE	H	H	7.42	0.02
134	1	16	ILE	HA	H	3.798	0.02
135	1	16	ILE	HB	H	1.918	0.02
136	1	16	ILE	HG12	H	1.493	0.02
137	1	16	ILE	HG13	H	1.208	0.02
138	1	16	ILE	HG21	H	0.922	0.02
139	1	16	ILE	HG22	H	0.922	0.02
140	1	16	ILE	HG23	H	0.922	0.02
141	1	16	ILE	HD11	H	0.678	0.02
142	1	16	ILE	HD12	H	0.678	0.02
143	1	16	ILE	HD13	H	0.678	0.02
144	1	16	ILE	C	C	178.647	0.3
145	1	16	ILE	CA	C	64.103	0.3
146	1	16	ILE	CB	C	37.774	0.3
147	1	16	ILE	CG1	C	29.188	0.3
148	1	16	ILE	CG2	C	17.305	0.3
149	1	16	ILE	CD1	C	12.863	0.3
150	1	16	ILE	N	N	121.53	0.3
151	1	17	LEU	H	H	8.116	0.02
152	1	17	LEU	HA	H	3.276	0.02
153	1	17	LEU	HB2	H	1.47	0.02
154	1	17	LEU	HB3	H	0.477	0.02
155	1	17	LEU	HG	H	1.581	0.02
156	1	17	LEU	HD11	H	0.587	0.02
157	1	17	LEU	HD12	H	0.587	0.02
158	1	17	LEU	HD13	H	0.587	0.02
159	1	17	LEU	HD21	H	0.789	0.02
160	1	17	LEU	HD22	H	0.789	0.02
161	1	17	LEU	HD23	H	0.789	0.02
162	1	17	LEU	C	C	178.328	0.3
163	1	17	LEU	CA	C	58.797	0.3
164	1	17	LEU	CB	C	39.865	0.3
165	1	17	LEU	CG	C	27.046	0.3
166	1	17	LEU	CD1	C	23.68	0.3
167	1	17	LEU	CD2	C	25.133	0.3
168	1	17	LEU	N	N	118.964	0.3
169	1	18	LYS	H	H	7.027	0.02
170	1	18	LYS	HA	H	3.902	0.02
171	1	18	LYS	HB2	H	1.856	0.02
172	1	18	LYS	HG2	H	1.391	0.02
173	1	18	LYS	HG3	H	1.57	0.02
174	1	18	LYS	HE2	H	2.991	0.02
175	1	18	LYS	C	C	178.574	0.3
176	1	18	LYS	CA	C	59.011	0.3
177	1	18	LYS	CB	C	32.397	0.3
178	1	18	LYS	CG	C	25.159	0.3
179	1	18	LYS	CD	C	29.217	0.3
180	1	18	LYS	CE	C	42.131	0.3
181	1	18	LYS	N	N	115.178	0.3
182	1	19	GLU	H	H	7.329	0.02
183	1	19	GLU	HA	H	4.204	0.02
184	1	19	GLU	HB2	H	2.164	0.02
185	1	19	GLU	HB3	H	2.066	0.02
186	1	19	GLU	HG2	H	2.24	0.02
187	1	19	GLU	HG3	H	2.404	0.02
188	1	19	GLU	C	C	178.828	0.3
189	1	19	GLU	CA	C	57.825	0.3
190	1	19	GLU	CB	C	30.455	0.3
191	1	19	GLU	CG	C	36.117	0.3
192	1	19	GLU	N	N	116.133	0.3
193	1	20	GLU	H	H	8.58	0.02
194	1	20	GLU	HA	H	4.338	0.02
195	1	20	GLU	HB2	H	1.758	0.02
196	1	20	GLU	HB3	H	2.26	0.02
197	1	20	GLU	HG2	H	2.516	0.02
198	1	20	GLU	C	C	179.347	0.3
199	1	20	GLU	CA	C	58.049	0.3
200	1	20	GLU	CB	C	30.673	0.3
201	1	20	GLU	CG	C	36.202	0.3
202	1	20	GLU	N	N	116.835	0.3
203	1	21	PHE	H	H	9.052	0.02
204	1	21	PHE	HA	H	4.685	0.02
205	1	21	PHE	HB2	H	3.575	0.02
206	1	21	PHE	HB3	H	3.24	0.02
207	1	21	PHE	CA	C	60.106	0.3
208	1	21	PHE	CB	C	34.279	0.3
209	1	21	PHE	N	N	119.56	0.3
210	1	22	PRO	HA	H	4.408	0.02
211	1	22	PRO	HB2	H	2.402	0.02
212	1	22	PRO	HB3	H	1.802	0.02
213	1	22	PRO	HG2	H	2.153	0.02
214	1	22	PRO	HG3	H	2.026	0.02
215	1	22	PRO	HD2	H	3.54	0.02
216	1	22	PRO	HD3	H	3.627	0.02
217	1	22	PRO	C	C	180.067	0.3
218	1	22	PRO	CA	C	66.244	0.3
219	1	22	PRO	CB	C	31.302	0.3
220	1	22	PRO	CG	C	28.742	0.3
221	1	22	PRO	CD	C	50.78	0.3
222	1	23	LYS	H	H	6.855	0.02
223	1	23	LYS	HA	H	4.114	0.02
224	1	23	LYS	HB2	H	2.142	0.02
225	1	23	LYS	HB3	H	1.963	0.02
226	1	23	LYS	HG2	H	1.423	0.02
227	1	23	LYS	HG3	H	1.65	0.02
228	1	23	LYS	HD2	H	1.746	0.02
229	1	23	LYS	HE2	H	2.955	0.02
230	1	23	LYS	C	C	179.872	0.3
231	1	23	LYS	CA	C	59.205	0.3
232	1	23	LYS	CB	C	32.273	0.3
233	1	23	LYS	CG	C	25.19	0.3
234	1	23	LYS	CD	C	29.695	0.3
235	1	23	LYS	CE	C	42.235	0.3
236	1	23	LYS	N	N	116.915	0.3
237	1	24	ILE	H	H	8.319	0.02
238	1	24	ILE	HA	H	3.534	0.02
239	1	24	ILE	HB	H	2.021	0.02
240	1	24	ILE	HG12	H	1.038	0.02
241	1	24	ILE	HG21	H	0.815	0.02
242	1	24	ILE	HG22	H	0.815	0.02
243	1	24	ILE	HG23	H	0.815	0.02
244	1	24	ILE	HD11	H	0.882	0.02
245	1	24	ILE	HD12	H	0.882	0.02
246	1	24	ILE	HD13	H	0.882	0.02
247	1	24	ILE	C	C	177.398	0.3
248	1	24	ILE	CA	C	65.647	0.3
249	1	24	ILE	CB	C	38.154	0.3
250	1	24	ILE	CG1	C	30.056	0.3
251	1	24	ILE	CG2	C	17.253	0.3
252	1	24	ILE	CD1	C	14.322	0.3
253	1	24	ILE	N	N	123.86	0.3
254	1	25	ASP	H	H	8.601	0.02
255	1	25	ASP	HA	H	4.233	0.02
256	1	25	ASP	HB2	H	2.657	0.02
257	1	25	ASP	HB3	H	2.538	0.02
258	1	25	ASP	C	C	179.169	0.3
259	1	25	ASP	CA	C	57.724	0.3
260	1	25	ASP	CB	C	40.09	0.3
261	1	25	ASP	N	N	120.311	0.3
262	1	26	SER	H	H	7.5	0.02
263	1	26	SER	HA	H	4.212	0.02
264	1	26	SER	HB2	H	3.931	0.02
265	1	26	SER	C	C	177.588	0.3
266	1	26	SER	CA	C	61.64	0.3
267	1	26	SER	CB	C	62.758	0.3
268	1	26	SER	N	N	114.026	0.3
269	1	27	LEU	H	H	7.961	0.02
270	1	27	LEU	HA	H	4.163	0.02
271	1	27	LEU	HB2	H	1.779	0.02
272	1	27	LEU	HB3	H	1.716	0.02
273	1	27	LEU	HG	H	1.663	0.02
274	1	27	LEU	HD11	H	0.786	0.02
275	1	27	LEU	HD12	H	0.786	0.02
276	1	27	LEU	HD13	H	0.786	0.02
277	1	27	LEU	HD21	H	0.928	0.02
278	1	27	LEU	HD22	H	0.928	0.02
279	1	27	LEU	HD23	H	0.928	0.02
280	1	27	LEU	C	C	178.718	0.3
281	1	27	LEU	CA	C	57.59	0.3
282	1	27	LEU	CB	C	42.587	0.3
283	1	27	LEU	CG	C	26.754	0.3
284	1	27	LEU	CD1	C	25.159	0.3
285	1	27	LEU	CD2	C	23.75	0.3
286	1	27	LEU	N	N	124.085	0.3
287	1	28	ALA	H	H	8.575	0.02
288	1	28	ALA	HA	H	3.57	0.02
289	1	28	ALA	HB1	H	1.264	0.02
290	1	28	ALA	HB2	H	1.264	0.02
291	1	28	ALA	HB3	H	1.264	0.02
292	1	28	ALA	C	C	177.334	0.3
293	1	28	ALA	CA	C	54.247	0.3
294	1	28	ALA	CB	C	18.655	0.3
295	1	28	ALA	N	N	119.154	0.3
296	1	29	GLN	H	H	7.111	0.02
297	1	29	GLN	HA	H	3.952	0.02
298	1	29	GLN	HB2	H	2.078	0.02
299	1	29	GLN	HG2	H	2.539	0.02
300	1	29	GLN	HG3	H	2.45	0.02
301	1	29	GLN	HE21	H	7.48	0.02
302	1	29	GLN	C	C	176.718	0.3
303	1	29	GLN	CA	C	58.028	0.3
304	1	29	GLN	CB	C	28.584	0.3
305	1	29	GLN	CG	C	33.787	0.3
306	1	29	GLN	N	N	113.04	0.3
307	1	29	GLN	NE2	N	112.98	0.3
308	1	30	ASN	H	H	7.479	0.02
309	1	30	ASN	HA	H	4.917	0.02
310	1	30	ASN	HB2	H	2.791	0.02
311	1	30	ASN	HB3	H	2.689	0.02
312	1	30	ASN	HD21	H	6.901	0.02
313	1	30	ASN	HD22	H	7.575	0.02
314	1	30	ASN	C	C	174.917	0.3
315	1	30	ASN	CA	C	53.801	0.3
316	1	30	ASN	CB	C	41.165	0.3
317	1	30	ASN	N	N	112.905	0.3
318	1	30	ASN	ND2	N	113.807	0.3
319	1	31	ASP	H	H	8.492	0.02
320	1	31	ASP	HA	H	4.707	0.02
321	1	31	ASP	HB2	H	2.812	0.02
322	1	31	ASP	HB3	H	2.516	0.02
323	1	31	ASP	C	C	174.393	0.3
324	1	31	ASP	CA	C	53.475	0.3
325	1	31	ASP	CB	C	40.549	0.3
326	1	31	ASP	N	N	120.058	0.3
327	1	32	CYS	H	H	8.867	0.02
328	1	32	CYS	HA	H	3.904	0.02
329	1	32	CYS	HB2	H	2.91	0.02
330	1	32	CYS	HB3	H	2.817	0.02
331	1	32	CYS	C	C	175.858	0.3
332	1	32	CYS	CA	C	62.678	0.3
333	1	32	CYS	CB	C	26.321	0.3
334	1	32	CYS	N	N	125.237	0.3
335	1	33	ASN	H	H	8.42	0.02
336	1	33	ASN	HA	H	4.309	0.02
337	1	33	ASN	HB2	H	2.832	0.02
338	1	33	ASN	HB3	H	2.692	0.02
339	1	33	ASN	HD21	H	6.88	0.02
340	1	33	ASN	HD22	H	7.712	0.02
341	1	33	ASN	C	C	177.674	0.3
342	1	33	ASN	CA	C	56.862	0.3
343	1	33	ASN	CB	C	37.736	0.3
344	1	33	ASN	N	N	117.778	0.3
345	1	33	ASN	ND2	N	112.975	0.3
346	1	34	SER	H	H	7.9	0.02
347	1	34	SER	HA	H	4.28	0.02
348	1	34	SER	HB2	H	3.691	0.02
349	1	34	SER	HB3	H	3.814	0.02
350	1	34	SER	C	C	176.727	0.3
351	1	34	SER	CA	C	61.643	0.3
352	1	34	SER	CB	C	62.385	0.3
353	1	34	SER	N	N	116.438	0.3
354	1	35	ALA	H	H	7.473	0.02
355	1	35	ALA	HA	H	3.917	0.02
356	1	35	ALA	HB1	H	1.393	0.02
357	1	35	ALA	HB2	H	1.393	0.02
358	1	35	ALA	HB3	H	1.393	0.02
359	1	35	ALA	C	C	178.404	0.3
360	1	35	ALA	CA	C	55.61	0.3
361	1	35	ALA	CB	C	18.441	0.3
362	1	35	ALA	N	N	123.61	0.3
363	1	36	LEU	H	H	8.285	0.02
364	1	36	LEU	HA	H	3.853	0.02
365	1	36	LEU	HB2	H	2.116	0.02
366	1	36	LEU	HB3	H	1.293	0.02
367	1	36	LEU	HG	H	1.55	0.02
368	1	36	LEU	C	C	178.07	0.3
369	1	36	LEU	CA	C	58.215	0.3
370	1	36	LEU	CB	C	40.785	0.3
371	1	36	LEU	N	N	118.946	0.3
372	1	37	ASP	H	H	8.033	0.02
373	1	37	ASP	HA	H	4.288	0.02
374	1	37	ASP	HB2	H	2.831	0.02
375	1	37	ASP	HB3	H	2.648	0.02
376	1	37	ASP	C	C	179.38	0.3
377	1	37	ASP	CA	C	57.899	0.3
378	1	37	ASP	CB	C	40.483	0.3
379	1	37	ASP	N	N	118.36	0.3
380	1	38	GLN	H	H	7.702	0.02
381	1	38	GLN	HA	H	4.026	0.02
382	1	38	GLN	HB2	H	2.086	0.02
383	1	38	GLN	HG2	H	2.461	0.02
384	1	38	GLN	C	C	180.109	0.3
385	1	38	GLN	CA	C	59.108	0.3
386	1	38	GLN	CB	C	28.133	0.3
387	1	38	GLN	CG	C	33.802	0.3
388	1	38	GLN	N	N	117.344	0.3
389	1	39	LEU	H	H	8.216	0.02
390	1	39	LEU	HA	H	4.069	0.02
391	1	39	LEU	HB2	H	1.259	0.02
392	1	39	LEU	HB3	H	2.153	0.02
393	1	39	LEU	HG	H	1.852	0.02
394	1	39	LEU	C	C	178.881	0.3
395	1	39	LEU	CA	C	58.3	0.3
396	1	39	LEU	CB	C	41.717	0.3
397	1	39	LEU	N	N	121.898	0.3
398	1	40	LEU	H	H	8.71	0.02
399	1	40	LEU	HA	H	4.17	0.02
400	1	40	LEU	HB2	H	1.384	0.02
401	1	40	LEU	HB3	H	2.049	0.02
402	1	40	LEU	HG	H	1.769	0.02
403	1	40	LEU	HD11	H	0.849	0.02
404	1	40	LEU	HD12	H	0.849	0.02
405	1	40	LEU	HD13	H	0.849	0.02
406	1	40	LEU	HD21	H	0.65	0.02
407	1	40	LEU	HD22	H	0.65	0.02
408	1	40	LEU	HD23	H	0.65	0.02
409	1	40	LEU	C	C	181.323	0.3
410	1	40	LEU	CA	C	57.629	0.3
411	1	40	LEU	CB	C	40.584	0.3
412	1	40	LEU	CG	C	26.708	0.3
413	1	40	LEU	CD1	C	25.506	0.3
414	1	40	LEU	CD2	C	21.755	0.3
415	1	40	LEU	N	N	120.202	0.3
416	1	41	VAL	H	H	7.775	0.02
417	1	41	VAL	HA	H	3.737	0.02
418	1	41	VAL	HB	H	2.265	0.02
419	1	41	VAL	HG11	H	0.902	0.02
420	1	41	VAL	HG12	H	0.902	0.02
421	1	41	VAL	HG13	H	0.902	0.02
422	1	41	VAL	HG21	H	1.071	0.02
423	1	41	VAL	HG22	H	1.071	0.02
424	1	41	VAL	HG23	H	1.071	0.02
425	1	41	VAL	C	C	178.738	0.3
426	1	41	VAL	CA	C	67.017	0.3
427	1	41	VAL	CB	C	31.828	0.3
428	1	41	VAL	CG1	C	21.242	0.3
429	1	41	VAL	CG2	C	22.624	0.3
430	1	41	VAL	N	N	123.804	0.3
431	1	42	LEU	H	H	7.293	0.02
432	1	42	LEU	HA	H	3.879	0.02
433	1	42	LEU	HB2	H	2.12	0.02
434	1	42	LEU	HB3	H	1.251	0.02
435	1	42	LEU	HG	H	1.89	0.02
436	1	42	LEU	HD11	H	0.889	0.02
437	1	42	LEU	HD12	H	0.889	0.02
438	1	42	LEU	HD13	H	0.889	0.02
439	1	42	LEU	HD21	H	0.738	0.02
440	1	42	LEU	HD22	H	0.738	0.02
441	1	42	LEU	HD23	H	0.738	0.02
442	1	42	LEU	C	C	179.255	0.3
443	1	42	LEU	CA	C	57.756	0.3
444	1	42	LEU	CB	C	42.285	0.3
445	1	42	LEU	CG	C	26.684	0.3
446	1	42	LEU	CD1	C	25.704	0.3
447	1	42	LEU	CD2	C	22.44	0.3
448	1	42	LEU	N	N	118.902	0.3
449	1	43	GLU	H	H	9.13	0.02
450	1	43	GLU	HA	H	3.561	0.02
451	1	43	GLU	HB2	H	1.754	0.02
452	1	43	GLU	HB3	H	2.5	0.02
453	1	43	GLU	HG2	H	2.251	0.02
454	1	43	GLU	HG3	H	2.2	0.02
455	1	43	GLU	C	C	177.065	0.3
456	1	43	GLU	CA	C	61.278	0.3
457	1	43	GLU	CB	C	28.734	0.3
458	1	43	GLU	CG	C	37.73	0.3
459	1	43	GLU	N	N	123.434	0.3
460	1	44	LYS	H	H	7.245	0.02
461	1	44	LYS	HA	H	3.835	0.02
462	1	44	LYS	HB2	H	1.97	0.02
463	1	44	LYS	HE2	H	2.988	0.02
464	1	44	LYS	C	C	178.477	0.3
465	1	44	LYS	CA	C	59.863	0.3
466	1	44	LYS	CB	C	32.166	0.3
467	1	44	LYS	N	N	119.316	0.3
468	1	45	LYS	H	H	7.687	0.02
469	1	45	LYS	HA	H	3.935	0.02
470	1	45	LYS	HB2	H	1.579	0.02
471	1	45	LYS	HB3	H	1.427	0.02
472	1	45	LYS	C	C	180.101	0.3
473	1	45	LYS	CA	C	59.822	0.3
474	1	45	LYS	CB	C	32.148	0.3
475	1	45	LYS	CG	C	25.093	0.3
476	1	45	LYS	N	N	117.313	0.3
477	1	46	THR	H	H	8.105	0.02
478	1	46	THR	HA	H	3.635	0.02
479	1	46	THR	HB	H	4.026	0.02
480	1	46	THR	HG21	H	0.911	0.02
481	1	46	THR	HG22	H	0.911	0.02
482	1	46	THR	HG23	H	0.911	0.02
483	1	46	THR	C	C	176.44	0.3
484	1	46	THR	CA	C	65.775	0.3
485	1	46	THR	CB	C	68.587	0.3
486	1	46	THR	CG2	C	23.277	0.3
487	1	46	THR	N	N	109.606	0.3
488	1	47	ARG	H	H	8.024	0.02
489	1	47	ARG	HA	H	4.01	0.02
490	1	47	ARG	HB2	H	2.083	0.02
491	1	47	ARG	HB3	H	1.977	0.02
492	1	47	ARG	C	C	180.658	0.3
493	1	47	ARG	CA	C	59.645	0.3
494	1	47	ARG	CB	C	29.97	0.3
495	1	47	ARG	N	N	125.124	0.3
496	1	48	GLN	H	H	8.692	0.02
497	1	48	GLN	HA	H	4.047	0.02
498	1	48	GLN	HB2	H	2.187	0.02
499	1	48	GLN	HB3	H	2.055	0.02
500	1	48	GLN	HG2	H	2.665	0.02
501	1	48	GLN	HG3	H	2.397	0.02
502	1	48	GLN	HE21	H	7.496	0.02
503	1	48	GLN	HE22	H	6.84	0.02
504	1	48	GLN	C	C	177.545	0.3
505	1	48	GLN	CA	C	59.1	0.3
506	1	48	GLN	CB	C	28.258	0.3
507	1	48	GLN	CG	C	34.728	0.3
508	1	48	GLN	N	N	119.449	0.3
509	1	48	GLN	NE2	N	111.549	0.3
510	1	49	ALA	H	H	7.321	0.02
511	1	49	ALA	HA	H	4.537	0.02
512	1	49	ALA	HB1	H	1.72	0.02
513	1	49	ALA	HB2	H	1.72	0.02
514	1	49	ALA	HB3	H	1.72	0.02
515	1	49	ALA	C	C	176.153	0.3
516	1	49	ALA	CA	C	51.962	0.3
517	1	49	ALA	CB	C	20.112	0.3
518	1	49	ALA	N	N	118.674	0.3
519	1	50	SER	H	H	7.96	0.02
520	1	50	SER	HA	H	3.843	0.02
521	1	50	SER	HB2	H	4.037	0.02
522	1	50	SER	HB3	H	4.174	0.02
523	1	50	SER	C	C	173.537	0.3
524	1	50	SER	CA	C	59.111	0.3
525	1	50	SER	CB	C	62.001	0.3
526	1	50	SER	N	N	113.121	0.3
527	1	51	ASP	H	H	8.146	0.02
528	1	51	ASP	HA	H	4.797	0.02
529	1	51	ASP	HB2	H	2.872	0.02
530	1	51	ASP	C	C	175.224	0.3
531	1	51	ASP	CA	C	51.774	0.3
532	1	51	ASP	CB	C	39.578	0.3
533	1	51	ASP	N	N	117.629	0.3
534	1	52	LEU	H	H	7.632	0.02
535	1	52	LEU	HA	H	3.903	0.02
536	1	52	LEU	HB2	H	1.799	0.02
537	1	52	LEU	HB3	H	1.563	0.02
538	1	52	LEU	HG	H	1.431	0.02
539	1	52	LEU	HD11	H	0.934	0.02
540	1	52	LEU	HD12	H	0.934	0.02
541	1	52	LEU	HD13	H	0.934	0.02
542	1	52	LEU	HD21	H	0.912	0.02
543	1	52	LEU	HD22	H	0.912	0.02
544	1	52	LEU	HD23	H	0.912	0.02
545	1	52	LEU	C	C	177.41	0.3
546	1	52	LEU	CA	C	58.406	0.3
547	1	52	LEU	CB	C	42.185	0.3
548	1	52	LEU	CG	C	27.257	0.3
549	1	52	LEU	CD1	C	26.051	0.3
550	1	52	LEU	CD2	C	24.674	0.3
551	1	52	LEU	N	N	125.711	0.3
552	1	53	ALA	H	H	8.446	0.02
553	1	53	ALA	HA	H	3.878	0.02
554	1	53	ALA	HB1	H	1.43	0.02
555	1	53	ALA	HB2	H	1.43	0.02
556	1	53	ALA	HB3	H	1.43	0.02
557	1	53	ALA	C	C	181.141	0.3
558	1	53	ALA	CA	C	55.677	0.3
559	1	53	ALA	CB	C	18.213	0.3
560	1	53	ALA	N	N	119.163	0.3
561	1	54	SER	H	H	7.527	0.02
562	1	54	SER	HA	H	4.232	0.02
563	1	54	SER	HB2	H	3.907	0.02
564	1	54	SER	HB3	H	4.008	0.02
565	1	54	SER	CA	C	61.258	0.3
566	1	54	SER	CB	C	62.209	0.3
567	1	54	SER	N	N	113.07	0.3
568	1	55	SER	H	H	8.563	0.02
569	1	55	SER	HA	H	4.007	0.02
570	1	55	SER	HB2	H	4.208	0.02
571	1	55	SER	C	C	176.842	0.3
572	1	55	SER	CA	C	62.114	0.3
573	1	55	SER	N	N	119.079	0.3
574	1	56	LYS	H	H	8.368	0.02
575	1	56	LYS	HA	H	3.847	0.02
576	1	56	LYS	HB2	H	2.19	0.02
577	1	56	LYS	HB3	H	1.748	0.02
578	1	56	LYS	C	C	178.437	0.3
579	1	56	LYS	CA	C	61.136	0.3
580	1	56	LYS	CB	C	32.582	0.3
581	1	56	LYS	N	N	116.931	0.3
582	1	57	GLU	H	H	7.027	0.02
583	1	57	GLU	HA	H	4.265	0.02
584	1	57	GLU	HB2	H	2.289	0.02
585	1	57	GLU	HG2	H	2.42	0.02
586	1	57	GLU	HG3	H	2.503	0.02
587	1	57	GLU	C	C	180.466	0.3
588	1	57	GLU	CA	C	59.182	0.3
589	1	57	GLU	CB	C	30.071	0.3
590	1	57	GLU	CG	C	35.957	0.3
591	1	57	GLU	N	N	114.666	0.3
592	1	58	VAL	H	H	8.508	0.02
593	1	58	VAL	HA	H	3.658	0.02
594	1	58	VAL	HB	H	2.33	0.02
595	1	58	VAL	HG11	H	0.91	0.02
596	1	58	VAL	HG12	H	0.91	0.02
597	1	58	VAL	HG13	H	0.91	0.02
598	1	58	VAL	HG21	H	1.124	0.02
599	1	58	VAL	HG22	H	1.124	0.02
600	1	58	VAL	HG23	H	1.124	0.02
601	1	58	VAL	C	C	177.667	0.3
602	1	58	VAL	CA	C	67.707	0.3
603	1	58	VAL	CB	C	31.524	0.3
604	1	58	VAL	CG1	C	22.549	0.3
605	1	58	VAL	CG2	C	24.949	0.3
606	1	58	VAL	N	N	121.297	0.3
607	1	59	LEU	H	H	8.974	0.02
608	1	59	LEU	HA	H	3.928	0.02
609	1	59	LEU	HB2	H	1.455	0.02
610	1	59	LEU	HB3	H	1.894	0.02
611	1	59	LEU	HG	H	2.111	0.02
612	1	59	LEU	HD11	H	0.827	0.02
613	1	59	LEU	HD12	H	0.827	0.02
614	1	59	LEU	HD13	H	0.827	0.02
615	1	59	LEU	HD21	H	0.843	0.02
616	1	59	LEU	HD22	H	0.843	0.02
617	1	59	LEU	HD23	H	0.843	0.02
618	1	59	LEU	C	C	180.132	0.3
619	1	59	LEU	CA	C	59.011	0.3
620	1	59	LEU	CB	C	39.677	0.3
621	1	59	LEU	CG	C	26.805	0.3
622	1	59	LEU	CD1	C	26.324	0.3
623	1	59	LEU	CD2	C	24.122	0.3
624	1	59	LEU	N	N	118.928	0.3
625	1	60	ALA	H	H	8.511	0.02
626	1	60	ALA	HA	H	3.813	0.02
627	1	60	ALA	HB1	H	1.659	0.02
628	1	60	ALA	HB2	H	1.659	0.02
629	1	60	ALA	HB3	H	1.659	0.02
630	1	60	ALA	C	C	179.159	0.3
631	1	60	ALA	CA	C	55.687	0.3
632	1	60	ALA	CB	C	18.974	0.3
633	1	60	ALA	N	N	119.366	0.3
634	1	61	LYS	H	H	7.84	0.02
635	1	61	LYS	HA	H	4.02	0.02
636	1	61	LYS	HB2	H	1.823	0.02
637	1	61	LYS	HB3	H	1.687	0.02
638	1	61	LYS	HG2	H	0.735	0.02
639	1	61	LYS	HG3	H	0.982	0.02
640	1	61	LYS	HD2	H	1.088	0.02
641	1	61	LYS	HD3	H	0.919	0.02
642	1	61	LYS	HE2	H	2.211	0.02
643	1	61	LYS	HE3	H	2.142	0.02
644	1	61	LYS	C	C	178.848	0.3
645	1	61	LYS	CA	C	58.123	0.3
646	1	61	LYS	CB	C	31.261	0.3
647	1	61	LYS	CG	C	24.924	0.3
648	1	61	LYS	CD	C	27.522	0.3
649	1	61	LYS	CE	C	41.265	0.3
650	1	61	LYS	N	N	119.838	0.3
651	1	62	ILE	H	H	8.077	0.02
652	1	62	ILE	HA	H	3.285	0.02
653	1	62	ILE	HB	H	2.047	0.02
654	1	62	ILE	HG12	H	1.99	0.02
655	1	62	ILE	HG21	H	0.54	0.02
656	1	62	ILE	HG22	H	0.54	0.02
657	1	62	ILE	HG23	H	0.54	0.02
658	1	62	ILE	HD11	H	0.66	0.02
659	1	62	ILE	HD12	H	0.66	0.02
660	1	62	ILE	HD13	H	0.66	0.02
661	1	62	ILE	C	C	176.871	0.3
662	1	62	ILE	CA	C	66.716	0.3
663	1	62	ILE	CB	C	37.594	0.3
664	1	62	ILE	CG1	C	31.175	0.3
665	1	62	ILE	CG2	C	15.914	0.3
666	1	62	ILE	CD1	C	13.768	0.3
667	1	62	ILE	N	N	120.941	0.3
668	1	63	VAL	H	H	7.536	0.02
669	1	63	VAL	HA	H	3.168	0.02
670	1	63	VAL	HB	H	2.167	0.02
671	1	63	VAL	HG11	H	0.592	0.02
672	1	63	VAL	HG12	H	0.592	0.02
673	1	63	VAL	HG13	H	0.592	0.02
674	1	63	VAL	HG21	H	1.028	0.02
675	1	63	VAL	HG22	H	1.028	0.02
676	1	63	VAL	HG23	H	1.028	0.02
677	1	63	VAL	C	C	176.853	0.3
678	1	63	VAL	CA	C	66.998	0.3
679	1	63	VAL	CB	C	31.096	0.3
680	1	63	VAL	CG1	C	21.628	0.3
681	1	63	VAL	CG2	C	24.297	0.3
682	1	63	VAL	N	N	117.465	0.3
683	1	64	ASP	H	H	8.172	0.02
684	1	64	ASP	HA	H	3.946	0.02
685	1	64	ASP	HB2	H	2.694	0.02
686	1	64	ASP	HB3	H	2.559	0.02
687	1	64	ASP	C	C	178.484	0.3
688	1	64	ASP	CA	C	57.864	0.3
689	1	64	ASP	CB	C	41.844	0.3
690	1	64	ASP	N	N	117.431	0.3
691	1	65	LEU	H	H	8.316	0.02
692	1	65	LEU	HA	H	3.63	0.02
693	1	65	LEU	HB2	H	1.71	0.02
694	1	65	LEU	HB3	H	1.436	0.02
695	1	65	LEU	HG	H	0.731	0.02
696	1	65	LEU	HD11	H	0.725	0.02
697	1	65	LEU	HD12	H	0.725	0.02
698	1	65	LEU	HD13	H	0.725	0.02
699	1	65	LEU	C	C	177.876	0.3
700	1	65	LEU	CA	C	58.031	0.3
701	1	65	LEU	CB	C	42.583	0.3
702	1	65	LEU	CG	C	26.651	0.3
703	1	65	LEU	CD1	C	24.638	0.3
704	1	65	LEU	N	N	120.02	0.3
705	1	66	LEU	H	H	7.367	0.02
706	1	66	LEU	HA	H	3.884	0.02
707	1	66	LEU	HB2	H	1.254	0.02
708	1	66	LEU	HB3	H	1.409	0.02
709	1	66	LEU	HD11	H	0.536	0.02
710	1	66	LEU	HD12	H	0.536	0.02
711	1	66	LEU	HD13	H	0.536	0.02
712	1	66	LEU	C	C	179.068	0.3
713	1	66	LEU	CA	C	56.675	0.3
714	1	66	LEU	CB	C	42.194	0.3
715	1	66	LEU	N	N	112.998	0.3
716	1	67	ALA	H	H	9.091	0.02
717	1	67	ALA	HA	H	2.443	0.02
718	1	67	ALA	HB1	H	0.252	0.02
719	1	67	ALA	HB2	H	0.252	0.02
720	1	67	ALA	HB3	H	0.252	0.02
721	1	67	ALA	C	C	181.628	0.3
722	1	67	ALA	CA	C	55.161	0.3
723	1	67	ALA	CB	C	17.426	0.3
724	1	67	ALA	N	N	122.58	0.3
725	1	68	SER	H	H	7.396	0.02
726	1	68	SER	HA	H	4.107	0.02
727	1	68	SER	C	C	175.103	0.3
728	1	68	SER	CA	C	61.567	0.3
729	1	68	SER	CB	C	62.725	0.3
730	1	68	SER	N	N	111.168	0.3
731	1	69	ARG	H	H	6.367	0.02
732	1	69	ARG	HA	H	4.364	0.02
733	1	69	ARG	HB2	H	2.102	0.02
734	1	69	ARG	HB3	H	1.237	0.02
735	1	69	ARG	HD2	H	3.905	0.02
736	1	69	ARG	C	C	174.66	0.3
737	1	69	ARG	CA	C	55.087	0.3
738	1	69	ARG	CB	C	30.551	0.3
739	1	69	ARG	CD	C	45.4	0.3
740	1	69	ARG	N	N	119.248	0.3
741	1	70	ASN	H	H	7.727	0.02
742	1	70	ASN	HA	H	3.815	0.02
743	1	70	ASN	HB2	H	3.323	0.02
744	1	70	ASN	HB3	H	2.928	0.02
745	1	70	ASN	HD21	H	6.941	0.02
746	1	70	ASN	HD22	H	7.674	0.02
747	1	70	ASN	C	C	175.068	0.3
748	1	70	ASN	CA	C	54.764	0.3
749	1	70	ASN	CB	C	37.442	0.3
750	1	70	ASN	N	N	114.729	0.3
751	1	70	ASN	ND2	N	114.355	0.3
752	1	71	LYS	H	H	7.812	0.02
753	1	71	LYS	HA	H	4.631	0.02
754	1	71	LYS	HB2	H	1.974	0.02
755	1	71	LYS	HB3	H	1.438	0.02
756	1	71	LYS	HG2	H	1.266	0.02
757	1	71	LYS	HD2	H	1.56	0.02
758	1	71	LYS	HE2	H	3.06	0.02
759	1	71	LYS	C	C	177.189	0.3
760	1	71	LYS	CA	C	54.243	0.3
761	1	71	LYS	CB	C	29.629	0.3
762	1	71	LYS	CG	C	24.029	0.3
763	1	71	LYS	CD	C	29.276	0.3
764	1	71	LYS	CE	C	42	0.3
765	1	71	LYS	N	N	120.991	0.3
766	1	72	TRP	H	H	7.048	0.02
767	1	72	TRP	HA	H	3.904	0.02
768	1	72	TRP	HB2	H	3.012	0.02
769	1	72	TRP	HB3	H	2.878	0.02
770	1	72	TRP	HD1	H	7.209	0.02
771	1	72	TRP	HE1	H	10.198	0.02
772	1	72	TRP	C	C	177.849	0.3
773	1	72	TRP	CA	C	60.216	0.3
774	1	72	TRP	CB	C	29.311	0.3
775	1	72	TRP	N	N	120.098	0.3
776	1	72	TRP	NE1	N	129.26	0.3
777	1	73	ASP	H	H	8.454	0.02
778	1	73	ASP	HA	H	4.512	0.02
779	1	73	ASP	HB2	H	2.686	0.02
780	1	73	ASP	C	C	179.519	0.3
781	1	73	ASP	CA	C	57.733	0.3
782	1	73	ASP	CB	C	39.75	0.3
783	1	73	ASP	N	N	118.418	0.3
784	1	74	ASP	H	H	8.067	0.02
785	1	74	ASP	HA	H	4.353	0.02
786	1	74	ASP	HB2	H	2.588	0.02
787	1	74	ASP	HB3	H	2.461	0.02
788	1	74	ASP	C	C	178.179	0.3
789	1	74	ASP	CA	C	57.249	0.3
790	1	74	ASP	CB	C	40.785	0.3
791	1	74	ASP	N	N	120.716	0.3
792	1	75	LEU	H	H	8.121	0.02
793	1	75	LEU	HA	H	3.78	0.02
794	1	75	LEU	HB2	H	2.05	0.02
795	1	75	LEU	HB3	H	1.108	0.02
796	1	75	LEU	HG	H	0.852	0.02
797	1	75	LEU	HD11	H	0.793	0.02
798	1	75	LEU	HD12	H	0.793	0.02
799	1	75	LEU	HD13	H	0.793	0.02
800	1	75	LEU	C	C	177.498	0.3
801	1	75	LEU	CA	C	58.215	0.3
802	1	75	LEU	CB	C	40.915	0.3
803	1	75	LEU	CG	C	26.73	0.3
804	1	75	LEU	CD1	C	24.073	0.3
805	1	75	LEU	N	N	122.004	0.3
806	1	76	ASN	H	H	8.672	0.02
807	1	76	ASN	HA	H	4.527	0.02
808	1	76	ASN	HB2	H	2.996	0.02
809	1	76	ASN	HB3	H	2.826	0.02
810	1	76	ASN	C	C	178.725	0.3
811	1	76	ASN	CA	C	56.126	0.3
812	1	76	ASN	CB	C	37.766	0.3
813	1	76	ASN	N	N	120.121	0.3
814	1	77	GLU	H	H	7.846	0.02
815	1	77	GLU	HA	H	4.04	0.02
816	1	77	GLU	HB2	H	2.095	0.02
817	1	77	GLU	HB3	H	2.202	0.02
818	1	77	GLU	HG2	H	2.143	0.02
819	1	77	GLU	HG3	H	2.235	0.02
820	1	77	GLU	C	C	178.69	0.3
821	1	77	GLU	CA	C	59.613	0.3
822	1	77	GLU	CB	C	29.306	0.3
823	1	77	GLU	CG	C	36.048	0.3
824	1	77	GLU	N	N	120.835	0.3
825	1	78	GLN	H	H	8.358	0.02
826	1	78	GLN	HA	H	4.005	0.02
827	1	78	GLN	HB2	H	2.12	0.02
828	1	78	GLN	HB3	H	1.765	0.02
829	1	78	GLN	HG2	H	2.616	0.02
830	1	78	GLN	HG3	H	1.85	0.02
831	1	78	GLN	C	C	178.444	0.3
832	1	78	GLN	CA	C	58.146	0.3
833	1	78	GLN	CB	C	26.986	0.3
834	1	78	GLN	CG	C	33.14	0.3
835	1	78	GLN	N	N	116.995	0.3
836	1	79	LEU	H	H	8.823	0.02
837	1	79	LEU	HA	H	3.89	0.02
838	1	79	LEU	HB2	H	1.778	0.02
839	1	79	LEU	HB3	H	1.578	0.02
840	1	79	LEU	HG	H	1.68	0.02
841	1	79	LEU	HD11	H	0.934	0.02
842	1	79	LEU	HD12	H	0.934	0.02
843	1	79	LEU	HD13	H	0.934	0.02
844	1	79	LEU	HD21	H	0.816	0.02
845	1	79	LEU	HD22	H	0.816	0.02
846	1	79	LEU	HD23	H	0.816	0.02
847	1	79	LEU	C	C	178.73	0.3
848	1	79	LEU	CA	C	59.082	0.3
849	1	79	LEU	CB	C	42.267	0.3
850	1	79	LEU	CG	C	27.476	0.3
851	1	79	LEU	CD1	C	24.65	0.3
852	1	79	LEU	CD2	C	24.919	0.3
853	1	79	LEU	N	N	120.091	0.3
854	1	80	THR	H	H	7.576	0.02
855	1	80	THR	HA	H	3.61	0.02
856	1	80	THR	HB	H	4.305	0.02
857	1	80	THR	HG21	H	1.129	0.02
858	1	80	THR	HG22	H	1.129	0.02
859	1	80	THR	HG23	H	1.129	0.02
860	1	80	THR	C	C	176.916	0.3
861	1	80	THR	CA	C	67.091	0.3
862	1	80	THR	CB	C	68.519	0.3
863	1	80	THR	CG2	C	21.252	0.3
864	1	80	THR	N	N	113.707	0.3
865	1	81	LEU	H	H	8.657	0.02
866	1	81	LEU	HA	H	3.909	0.02
867	1	81	LEU	HB2	H	1.768	0.02
868	1	81	LEU	HB3	H	1.333	0.02
869	1	81	LEU	HG	H	1.802	0.02
870	1	81	LEU	HD11	H	0.78	0.02
871	1	81	LEU	HD12	H	0.78	0.02
872	1	81	LEU	HD13	H	0.78	0.02
873	1	81	LEU	HD21	H	0.799	0.02
874	1	81	LEU	HD22	H	0.799	0.02
875	1	81	LEU	HD23	H	0.799	0.02
876	1	81	LEU	C	C	181.739	0.3
877	1	81	LEU	CA	C	58.128	0.3
878	1	81	LEU	CB	C	42.614	0.3
879	1	81	LEU	CG	C	26.257	0.3
880	1	81	LEU	CD1	C	25.812	0.3
881	1	81	LEU	CD2	C	22.439	0.3
882	1	81	LEU	N	N	121.017	0.3
883	1	82	LEU	H	H	9.385	0.02
884	1	82	LEU	HA	H	4.03	0.02
885	1	82	LEU	HB2	H	1.888	0.02
886	1	82	LEU	HB3	H	0.89	0.02
887	1	82	LEU	HG	H	2.01	0.02
888	1	82	LEU	C	C	181.077	0.3
889	1	82	LEU	CA	C	57.733	0.3
890	1	82	LEU	CB	C	40.836	0.3
891	1	82	LEU	CG	C	30.266	0.3
892	1	82	LEU	N	N	118.311	0.3
893	1	83	SER	H	H	7.786	0.02
894	1	83	SER	HA	H	4.008	0.02
895	1	83	SER	HB2	H	3.959	0.02
896	1	83	SER	C	C	174.337	0.3
897	1	83	SER	CA	C	61.767	0.3
898	1	83	SER	CB	C	62.934	0.3
899	1	83	SER	N	N	114.055	0.3
900	1	84	LYS	H	H	6.881	0.02
901	1	84	LYS	HA	H	4.428	0.02
902	1	84	LYS	HB2	H	2.05	0.02
903	1	84	LYS	HB3	H	1.657	0.02
904	1	84	LYS	HG2	H	1.426	0.02
905	1	84	LYS	HG3	H	1.626	0.02
906	1	84	LYS	HD2	H	1.641	0.02
907	1	84	LYS	HE2	H	2.935	0.02
908	1	84	LYS	C	C	176.658	0.3
909	1	84	LYS	CA	C	55.886	0.3
910	1	84	LYS	CB	C	33.622	0.3
911	1	84	LYS	CG	C	25.025	0.3
912	1	84	LYS	CD	C	29.319	0.3
913	1	84	LYS	CE	C	42.165	0.3
914	1	84	LYS	N	N	118.235	0.3
915	1	85	LYS	H	H	6.88	0.02
916	1	85	LYS	HA	H	4.181	0.02
917	1	85	LYS	HB2	H	1.789	0.02
918	1	85	LYS	HB3	H	1.586	0.02
919	1	85	LYS	C	C	177.701	0.3
920	1	85	LYS	CA	C	54.98	0.3
921	1	85	LYS	CB	C	33.836	0.3
922	1	85	LYS	N	N	118.235	0.3
923	1	86	HIS	H	H	9.445	0.02
924	1	86	HIS	HA	H	4.384	0.02
925	1	86	HIS	HB2	H	3.234	0.02
926	1	86	HIS	HB3	H	3.163	0.02
927	1	86	HIS	C	C	176.976	0.3
928	1	86	HIS	CA	C	57.548	0.3
929	1	86	HIS	CB	C	28.703	0.3
930	1	86	HIS	N	N	123.188	0.3
931	1	87	GLY	H	H	9.11	0.02
932	1	87	GLY	HA2	H	3.843	0.02
933	1	87	GLY	HA3	H	3.586	0.02
934	1	87	GLY	C	C	174.088	0.3
935	1	87	GLY	CA	C	45.252	0.3
936	1	87	GLY	N	N	115.322	0.3
937	1	88	GLN	H	H	7.25	0.02
938	1	88	GLN	HA	H	3.932	0.02
939	1	88	GLN	HB2	H	1.995	0.02
940	1	88	GLN	C	C	175.054	0.3
941	1	88	GLN	CA	C	55.558	0.3
942	1	88	GLN	CB	C	30.355	0.3
943	1	88	GLN	CG	C	36.202	0.3
944	1	88	GLN	N	N	117.502	0.3
945	1	89	LEU	H	H	9.124	0.02
946	1	89	LEU	HA	H	4.457	0.02
947	1	89	LEU	HB2	H	2.015	0.02
948	1	89	LEU	HB3	H	1.536	0.02
949	1	89	LEU	HG	H	1.871	0.02
950	1	89	LEU	HD11	H	0.976	0.02
951	1	89	LEU	HD12	H	0.976	0.02
952	1	89	LEU	HD13	H	0.976	0.02
953	1	89	LEU	HD21	H	0.943	0.02
954	1	89	LEU	HD22	H	0.943	0.02
955	1	89	LEU	HD23	H	0.943	0.02
956	1	89	LEU	C	C	177.607	0.3
957	1	89	LEU	CA	C	54.255	0.3
958	1	89	LEU	CB	C	41.163	0.3
959	1	89	LEU	CG	C	27.25	0.3
960	1	89	LEU	CD1	C	25.794	0.3
961	1	89	LEU	CD2	C	24.004	0.3
962	1	89	LEU	N	N	129.983	0.3
963	1	90	LYS	H	H	8.859	0.02
964	1	90	LYS	HA	H	3.718	0.02
965	1	90	LYS	HB2	H	1.831	0.02
966	1	90	LYS	HB3	H	1.747	0.02
967	1	90	LYS	HG2	H	1.398	0.02
968	1	90	LYS	HG3	H	1.283	0.02
969	1	90	LYS	HD2	H	1.642	0.02
970	1	90	LYS	HD3	H	1.554	0.02
971	1	90	LYS	HE2	H	2.903	0.02
972	1	90	LYS	C	C	179.008	0.3
973	1	90	LYS	CA	C	60.322	0.3
974	1	90	LYS	CB	C	32.122	0.3
975	1	90	LYS	CG	C	25.119	0.3
976	1	90	LYS	CD	C	29.266	0.3
977	1	90	LYS	CE	C	42.159	0.3
978	1	90	LYS	N	N	128.061	0.3
979	1	91	LEU	H	H	8.768	0.02
980	1	91	LEU	HA	H	4.092	0.02
981	1	91	LEU	HB2	H	1.738	0.02
982	1	91	LEU	HB3	H	1.48	0.02
983	1	91	LEU	HG	H	1.738	0.02
984	1	91	LEU	HD11	H	0.887	0.02
985	1	91	LEU	HD12	H	0.887	0.02
986	1	91	LEU	HD13	H	0.887	0.02
987	1	91	LEU	HD21	H	0.939	0.02
988	1	91	LEU	HD22	H	0.939	0.02
989	1	91	LEU	HD23	H	0.939	0.02
990	1	91	LEU	C	C	179.995	0.3
991	1	91	LEU	CA	C	57.649	0.3
992	1	91	LEU	CB	C	41.936	0.3
993	1	91	LEU	CG	C	27.966	0.3
994	1	91	LEU	CD1	C	22.851	0.3
995	1	91	LEU	CD2	C	25.123	0.3
996	1	91	LEU	N	N	115.881	0.3
997	1	92	SER	H	H	7.215	0.02
998	1	92	SER	HA	H	4.197	0.02
999	1	92	SER	HB2	H	3.858	0.02
1000	1	92	SER	C	C	176.413	0.3
1001	1	92	SER	CA	C	61.624	0.3
1002	1	92	SER	CB	C	62.638	0.3
1003	1	92	SER	N	N	116.912	0.3
1004	1	93	ILE	H	H	7.278	0.02
1005	1	93	ILE	HA	H	3.687	0.02
1006	1	93	ILE	HB	H	2.05	0.02
1007	1	93	ILE	HG12	H	1.357	0.02
1008	1	93	ILE	HG13	H	1.294	0.02
1009	1	93	ILE	HG21	H	0.77	0.02
1010	1	93	ILE	HG22	H	0.77	0.02
1011	1	93	ILE	HG23	H	0.77	0.02
1012	1	93	ILE	HD11	H	0.547	0.02
1013	1	93	ILE	HD12	H	0.547	0.02
1014	1	93	ILE	HD13	H	0.547	0.02
1015	1	93	ILE	C	C	177.987	0.3
1016	1	93	ILE	CA	C	61.735	0.3
1017	1	93	ILE	CB	C	35.6	0.3
1018	1	93	ILE	CG1	C	27.825	0.3
1019	1	93	ILE	CG2	C	16.988	0.3
1020	1	93	ILE	CD1	C	8.83	0.3
1021	1	93	ILE	N	N	121.101	0.3
1022	1	94	GLN	H	H	8.345	0.02
1023	1	94	GLN	HA	H	3.84	0.02
1024	1	94	GLN	HB2	H	2.113	0.02
1025	1	94	GLN	HG2	H	2.462	0.02
1026	1	94	GLN	HG3	H	2.296	0.02
1027	1	94	GLN	HE21	H	6.734	0.02
1028	1	94	GLN	HE22	H	7.384	0.02
1029	1	94	GLN	C	C	178.097	0.3
1030	1	94	GLN	CA	C	59.896	0.3
1031	1	94	GLN	CB	C	28.613	0.3
1032	1	94	GLN	CG	C	34.389	0.3
1033	1	94	GLN	N	N	118.48	0.3
1034	1	94	GLN	NE2	N	110.442	0.3
1035	1	95	TYR	H	H	7.58	0.02
1036	1	95	TYR	HA	H	4.363	0.02
1037	1	95	TYR	HB2	H	3.276	0.02
1038	1	95	TYR	HB3	H	3.217	0.02
1039	1	95	TYR	C	C	176.81	0.3
1040	1	95	TYR	CA	C	61.222	0.3
1041	1	95	TYR	CB	C	38.95	0.3
1042	1	95	TYR	N	N	120.915	0.3
1043	1	96	MET	H	H	8.503	0.02
1044	1	96	MET	HA	H	3.482	0.02
1045	1	96	MET	HB2	H	2.256	0.02
1046	1	96	MET	HB3	H	2.075	0.02
1047	1	96	MET	C	C	178.032	0.3
1048	1	96	MET	CA	C	60.742	0.3
1049	1	96	MET	CB	C	32.294	0.3
1050	1	96	MET	N	N	119.88	0.3
1051	1	97	ILE	H	H	8.43	0.02
1052	1	97	ILE	HA	H	3.366	0.02
1053	1	97	ILE	HB	H	1.956	0.02
1054	1	97	ILE	HG12	H	1.783	0.02
1055	1	97	ILE	HG21	H	0.844	0.02
1056	1	97	ILE	HG22	H	0.844	0.02
1057	1	97	ILE	HG23	H	0.844	0.02
1058	1	97	ILE	HD11	H	0.742	0.02
1059	1	97	ILE	HD12	H	0.742	0.02
1060	1	97	ILE	HD13	H	0.742	0.02
1061	1	97	ILE	C	C	177.347	0.3
1062	1	97	ILE	CA	C	65.948	0.3
1063	1	97	ILE	CB	C	37.754	0.3
1064	1	97	ILE	CG1	C	30.458	0.3
1065	1	97	ILE	CG2	C	18.05	0.3
1066	1	97	ILE	CD1	C	13.941	0.3
1067	1	97	ILE	N	N	117.679	0.3
1068	1	98	GLN	H	H	7.966	0.02
1069	1	98	GLN	HA	H	3.926	0.02
1070	1	98	GLN	HB2	H	2.126	0.02
1071	1	98	GLN	HB3	H	2.268	0.02
1072	1	98	GLN	HG2	H	2.474	0.02
1073	1	98	GLN	HG3	H	2.284	0.02
1074	1	98	GLN	HE21	H	6.82	0.02
1075	1	98	GLN	HE22	H	7.521	0.02
1076	1	98	GLN	C	C	179.34	0.3
1077	1	98	GLN	CA	C	59.616	0.3
1078	1	98	GLN	CB	C	27.989	0.3
1079	1	98	GLN	CG	C	33.832	0.3
1080	1	98	GLN	N	N	120.429	0.3
1081	1	98	GLN	NE2	N	111.232	0.3
1082	1	99	LYS	H	H	8	0.02
1083	1	99	LYS	HA	H	4.039	0.02
1084	1	99	LYS	HB2	H	1.787	0.02
1085	1	99	LYS	HB3	H	1.998	0.02
1086	1	99	LYS	HG2	H	1.148	0.02
1087	1	99	LYS	HG3	H	1.05	0.02
1088	1	99	LYS	HD2	H	1.5	0.02
1089	1	99	LYS	HD3	H	1.563	0.02
1090	1	99	LYS	HE2	H	2.779	0.02
1091	1	99	LYS	HE3	H	2.644	0.02
1092	1	99	LYS	C	C	179.23	0.3
1093	1	99	LYS	CA	C	57.439	0.3
1094	1	99	LYS	CB	C	30.314	0.3
1095	1	99	LYS	CG	C	23.64	0.3
1096	1	99	LYS	CD	C	26.641	0.3
1097	1	99	LYS	CE	C	41.928	0.3
1098	1	99	LYS	N	N	118.495	0.3
1099	1	100	VAL	H	H	8.527	0.02
1100	1	100	VAL	HA	H	3.497	0.02
1101	1	100	VAL	HB	H	2.412	0.02
1102	1	100	VAL	HG11	H	1.026	0.02
1103	1	100	VAL	HG12	H	1.026	0.02
1104	1	100	VAL	HG13	H	1.026	0.02
1105	1	100	VAL	HG21	H	1.174	0.02
1106	1	100	VAL	HG22	H	1.174	0.02
1107	1	100	VAL	HG23	H	1.174	0.02
1108	1	100	VAL	C	C	178.196	0.3
1109	1	100	VAL	CA	C	67.995	0.3
1110	1	100	VAL	CB	C	31.47	0.3
1111	1	100	VAL	CG1	C	22.792	0.3
1112	1	100	VAL	CG2	C	24.482	0.3
1113	1	100	VAL	N	N	121.176	0.3
1114	1	101	MET	H	H	8.511	0.02
1115	1	101	MET	HA	H	4.164	0.02
1116	1	101	MET	HB2	H	2.264	0.02
1117	1	101	MET	HB3	H	2.031	0.02
1118	1	101	MET	HG2	H	2.503	0.02
1119	1	101	MET	HG3	H	2.744	0.02
1120	1	101	MET	C	C	178.98	0.3
1121	1	101	MET	CA	C	58.159	0.3
1122	1	101	MET	CB	C	30.864	0.3
1123	1	101	MET	CG	C	32.403	0.3
1124	1	101	MET	N	N	116.772	0.3
1125	1	102	GLU	H	H	7.998	0.02
1126	1	102	GLU	HA	H	3.937	0.02
1127	1	102	GLU	HB2	H	2.206	0.02
1128	1	102	GLU	HB3	H	2	0.02
1129	1	102	GLU	HG2	H	2.129	0.02
1130	1	102	GLU	HG3	H	2.455	0.02
1131	1	102	GLU	C	C	179.948	0.3
1132	1	102	GLU	CA	C	59.686	0.3
1133	1	102	GLU	CB	C	29.41	0.3
1134	1	102	GLU	CG	C	36.176	0.3
1135	1	102	GLU	N	N	120.325	0.3
1136	1	103	TYR	H	H	7.975	0.02
1137	1	103	TYR	HA	H	4.21	0.02
1138	1	103	TYR	HB2	H	3.138	0.02
1139	1	103	TYR	HB3	H	3.084	0.02
1140	1	103	TYR	C	C	179.548	0.3
1141	1	103	TYR	CA	C	62.521	0.3
1142	1	103	TYR	CB	C	38.695	0.3
1143	1	103	TYR	N	N	119.298	0.3
1144	1	104	LEU	H	H	8.801	0.02
1145	1	104	LEU	HA	H	3.899	0.02
1146	1	104	LEU	HB2	H	1.835	0.02
1147	1	104	LEU	HB3	H	1.509	0.02
1148	1	104	LEU	HG	H	1.641	0.02
1149	1	104	LEU	HD11	H	0.587	0.02
1150	1	104	LEU	HD12	H	0.587	0.02
1151	1	104	LEU	HD13	H	0.587	0.02
1152	1	104	LEU	HD21	H	0.495	0.02
1153	1	104	LEU	HD22	H	0.495	0.02
1154	1	104	LEU	HD23	H	0.495	0.02
1155	1	104	LEU	C	C	179.441	0.3
1156	1	104	LEU	CA	C	58.343	0.3
1157	1	104	LEU	CB	C	42.246	0.3
1158	1	104	LEU	CG	C	26.881	0.3
1159	1	104	LEU	CD1	C	25.184	0.3
1160	1	104	LEU	CD2	C	24.012	0.3
1161	1	104	LEU	N	N	121.538	0.3
1162	1	105	LYS	H	H	7.91	0.02
1163	1	105	LYS	HA	H	4.046	0.02
1164	1	105	LYS	HB2	H	1.871	0.02
1165	1	105	LYS	HG2	H	1.516	0.02
1166	1	105	LYS	HG3	H	1.401	0.02
1167	1	105	LYS	HD2	H	1.639	0.02
1168	1	105	LYS	HE2	H	2.939	0.02
1169	1	105	LYS	C	C	178.007	0.3
1170	1	105	LYS	CA	C	58.617	0.3
1171	1	105	LYS	CB	C	32.671	0.3
1172	1	105	LYS	CG	C	25.16	0.3
1173	1	105	LYS	CD	C	29.149	0.3
1174	1	105	LYS	CE	C	42.199	0.3
1175	1	105	LYS	N	N	117.335	0.3
1176	1	106	SER	H	H	7.552	0.02
1177	1	106	SER	HA	H	4.41	0.02
1178	1	106	SER	HB2	H	3.922	0.02
1179	1	106	SER	HB3	H	3.845	0.02
1180	1	106	SER	C	C	174.972	0.3
1181	1	106	SER	CA	C	59.08	0.3
1182	1	106	SER	CB	C	64.26	0.3
1183	1	106	SER	N	N	111.696	0.3
1184	1	107	SER	H	H	7.622	0.02
1185	1	107	SER	HA	H	4.538	0.02
1186	1	107	SER	HB2	H	4.154	0.02
1187	1	107	SER	HB3	H	3.956	0.02
1188	1	107	SER	C	C	175.795	0.3
1189	1	107	SER	CA	C	58.584	0.3
1190	1	107	SER	CB	C	63.376	0.3
1191	1	107	SER	N	N	117.47	0.3
1192	1	108	LYS	H	H	8.66	0.02
1193	1	108	LYS	HA	H	4.348	0.02
1194	1	108	LYS	HB2	H	1.965	0.02
1195	1	108	LYS	HB3	H	1.776	0.02
1196	1	108	LYS	HG2	H	1.54	0.02
1197	1	108	LYS	HG3	H	1.471	0.02
1198	1	108	LYS	HD2	H	1.688	0.02
1199	1	108	LYS	HE2	H	3.017	0.02
1200	1	108	LYS	C	C	177.31	0.3
1201	1	108	LYS	CA	C	56.973	0.3
1202	1	108	LYS	CB	C	32.746	0.3
1203	1	108	LYS	CG	C	24.966	0.3
1204	1	108	LYS	CD	C	28.764	0.3
1205	1	108	LYS	CE	C	42.165	0.3
1206	1	108	LYS	N	N	125.453	0.3
1207	1	109	SER	H	H	8.307	0.02
1208	1	109	SER	HA	H	4.486	0.02
1209	1	109	SER	HB2	H	3.948	0.02
1210	1	109	SER	HB3	H	3.873	0.02
1211	1	109	SER	C	C	173.996	0.3
1212	1	109	SER	CA	C	58.019	0.3
1213	1	109	SER	CB	C	63.831	0.3
1214	1	109	SER	N	N	113.374	0.3
1215	1	110	LEU	H	H	7.289	0.02
1216	1	110	LEU	HA	H	4.204	0.02
1217	1	110	LEU	HB2	H	1.355	0.02
1218	1	110	LEU	HG	H	1.371	0.02
1219	1	110	LEU	HD11	H	0.108	0.02
1220	1	110	LEU	HD12	H	0.108	0.02
1221	1	110	LEU	HD13	H	0.108	0.02
1222	1	110	LEU	HD21	H	0.333	0.02
1223	1	110	LEU	HD22	H	0.333	0.02
1224	1	110	LEU	HD23	H	0.333	0.02
1225	1	110	LEU	C	C	176.508	0.3
1226	1	110	LEU	CA	C	55.296	0.3
1227	1	110	LEU	CB	C	43.223	0.3
1228	1	110	LEU	CG	C	26.06	0.3
1229	1	110	LEU	CD1	C	24.919	0.3
1230	1	110	LEU	CD2	C	24.14	0.3
1231	1	110	LEU	N	N	123.876	0.3
1232	1	111	ASP	H	H	8.341	0.02
1233	1	111	ASP	HA	H	4.486	0.02
1234	1	111	ASP	HB2	H	2.885	0.02
1235	1	111	ASP	HB3	H	2.697	0.02
1236	1	111	ASP	C	C	175.977	0.3
1237	1	111	ASP	CA	C	53.632	0.3
1238	1	111	ASP	CB	C	42.024	0.3
1239	1	111	ASP	N	N	125.324	0.3
1240	1	112	LEU	H	H	8.477	0.02
1241	1	112	LEU	HA	H	3.941	0.02
1242	1	112	LEU	HB2	H	1.586	0.02
1243	1	112	LEU	HB3	H	1.633	0.02
1244	1	112	LEU	HG	H	1.58	0.02
1245	1	112	LEU	HD11	H	0.862	0.02
1246	1	112	LEU	HD12	H	0.862	0.02
1247	1	112	LEU	HD13	H	0.862	0.02
1248	1	112	LEU	HD21	H	0.887	0.02
1249	1	112	LEU	HD22	H	0.887	0.02
1250	1	112	LEU	HD23	H	0.887	0.02
1251	1	112	LEU	C	C	178.226	0.3
1252	1	112	LEU	CA	C	58.517	0.3
1253	1	112	LEU	CB	C	42.003	0.3
1254	1	112	LEU	CG	C	26.766	0.3
1255	1	112	LEU	CD1	C	24.974	0.3
1256	1	112	LEU	CD2	C	23.678	0.3
1257	1	112	LEU	N	N	123.482	0.3
1258	1	113	ASN	H	H	8.377	0.02
1259	1	113	ASN	HA	H	4.307	0.02
1260	1	113	ASN	HB2	H	2.696	0.02
1261	1	113	ASN	HB3	H	2.795	0.02
1262	1	113	ASN	HD21	H	6.877	0.02
1263	1	113	ASN	HD22	H	7.714	0.02
1264	1	113	ASN	C	C	178.727	0.3
1265	1	113	ASN	CA	C	56.605	0.3
1266	1	113	ASN	CB	C	37.649	0.3
1267	1	113	ASN	N	N	115.434	0.3
1268	1	113	ASN	ND2	N	113.041	0.3
1269	1	114	THR	H	H	8.073	0.02
1270	1	114	THR	HA	H	3.785	0.02
1271	1	114	THR	HB	H	3.86	0.02
1272	1	114	THR	HG21	H	0.839	0.02
1273	1	114	THR	HG22	H	0.839	0.02
1274	1	114	THR	HG23	H	0.839	0.02
1275	1	114	THR	C	C	176.387	0.3
1276	1	114	THR	CA	C	66.664	0.3
1277	1	114	THR	CB	C	67.401	0.3
1278	1	114	THR	CG2	C	22.221	0.3
1279	1	114	THR	N	N	119.228	0.3
1280	1	115	ARG	H	H	8.068	0.02
1281	1	115	ARG	HA	H	3.36	0.02
1282	1	115	ARG	HB2	H	1.848	0.02
1283	1	115	ARG	HB3	H	1.651	0.02
1284	1	115	ARG	HG2	H	1.318	0.02
1285	1	115	ARG	HG3	H	1.394	0.02
1286	1	115	ARG	HD2	H	2.97	0.02
1287	1	115	ARG	HD3	H	3.203	0.02
1288	1	115	ARG	HE	H	7.381	0.02
1289	1	115	ARG	HH21	H	6.67	0.02
1290	1	115	ARG	C	C	177.612	0.3
1291	1	115	ARG	CA	C	60.967	0.3
1292	1	115	ARG	CB	C	30.389	0.3
1293	1	115	ARG	CG	C	27.651	0.3
1294	1	115	ARG	CD	C	43.334	0.3
1295	1	115	ARG	N	N	122.976	0.3
1296	1	115	ARG	NE	N	86.58	0.3
1297	1	116	ILE	H	H	8.414	0.02
1298	1	116	ILE	HA	H	3.572	0.02
1299	1	116	ILE	HB	H	1.872	0.02
1300	1	116	ILE	HG12	H	1.147	0.02
1301	1	116	ILE	HG13	H	1.66	0.02
1302	1	116	ILE	HG21	H	0.862	0.02
1303	1	116	ILE	HG22	H	0.862	0.02
1304	1	116	ILE	HG23	H	0.862	0.02
1305	1	116	ILE	HD11	H	0.784	0.02
1306	1	116	ILE	HD12	H	0.784	0.02
1307	1	116	ILE	HD13	H	0.784	0.02
1308	1	116	ILE	C	C	177.914	0.3
1309	1	116	ILE	CA	C	64.983	0.3
1310	1	116	ILE	CB	C	37.707	0.3
1311	1	116	ILE	CG1	C	29.145	0.3
1312	1	116	ILE	CG2	C	17.305	0.3
1313	1	116	ILE	CD1	C	12.811	0.3
1314	1	116	ILE	N	N	117.133	0.3
1315	1	117	SER	H	H	7.678	0.02
1316	1	117	SER	HA	H	4.248	0.02
1317	1	117	SER	HB2	H	3.97	0.02
1318	1	117	SER	HB3	H	3.996	0.02
1319	1	117	SER	C	C	176.976	0.3
1320	1	117	SER	CA	C	61.812	0.3
1321	1	117	SER	CB	C	62.782	0.3
1322	1	117	SER	N	N	116.011	0.3
1323	1	118	VAL	H	H	7.824	0.02
1324	1	118	VAL	HA	H	4.398	0.02
1325	1	118	VAL	HB	H	2.034	0.02
1326	1	118	VAL	HG11	H	0.953	0.02
1327	1	118	VAL	HG12	H	0.953	0.02
1328	1	118	VAL	HG13	H	0.953	0.02
1329	1	118	VAL	HG21	H	1.095	0.02
1330	1	118	VAL	HG22	H	1.095	0.02
1331	1	118	VAL	HG23	H	1.095	0.02
1332	1	118	VAL	C	C	178.134	0.3
1333	1	118	VAL	CA	C	65.156	0.3
1334	1	118	VAL	CB	C	32.091	0.3
1335	1	118	VAL	CG1	C	22.127	0.3
1336	1	118	VAL	CG2	C	21.615	0.3
1337	1	118	VAL	N	N	126.141	0.3
1338	1	119	ILE	H	H	8	0.02
1339	1	119	ILE	HA	H	3.5	0.02
1340	1	119	ILE	HB	H	1.936	0.02
1341	1	119	ILE	HG12	H	1.073	0.02
1342	1	119	ILE	HG13	H	1.551	0.02
1343	1	119	ILE	HG21	H	0.835	0.02
1344	1	119	ILE	HG22	H	0.835	0.02
1345	1	119	ILE	HG23	H	0.835	0.02
1346	1	119	ILE	HD11	H	0.672	0.02
1347	1	119	ILE	HD12	H	0.672	0.02
1348	1	119	ILE	HD13	H	0.672	0.02
1349	1	119	ILE	C	C	178.925	0.3
1350	1	119	ILE	CA	C	65.147	0.3
1351	1	119	ILE	CB	C	37.14	0.3
1352	1	119	ILE	CG1	C	29.159	0.3
1353	1	119	ILE	CG2	C	17.271	0.3
1354	1	119	ILE	CD1	C	12.312	0.3
1355	1	119	ILE	N	N	120.623	0.3
1356	1	120	GLU	H	H	8.781	0.02
1357	1	120	GLU	HA	H	4.176	0.02
1358	1	120	GLU	HB2	H	2.061	0.02
1359	1	120	GLU	HB3	H	1.969	0.02
1360	1	120	GLU	HG2	H	2.296	0.02
1361	1	120	GLU	C	C	178.776	0.3
1362	1	120	GLU	CA	C	59.039	0.3
1363	1	120	GLU	CB	C	29.051	0.3
1364	1	120	GLU	CG	C	36.159	0.3
1365	1	120	GLU	N	N	119.861	0.3
1366	1	121	THR	H	H	7.765	0.02
1367	1	121	THR	HA	H	3.839	0.02
1368	1	121	THR	HB	H	4.441	0.02
1369	1	121	THR	HG21	H	1.169	0.02
1370	1	121	THR	HG22	H	1.169	0.02
1371	1	121	THR	HG23	H	1.169	0.02
1372	1	121	THR	C	C	176.263	0.3
1373	1	121	THR	CA	C	67.699	0.3
1374	1	121	THR	CB	C	68.251	0.3
1375	1	121	THR	CG2	C	21.751	0.3
1376	1	121	THR	N	N	118.498	0.3
1377	1	122	ILE	H	H	8.291	0.02
1378	1	122	ILE	HA	H	3.351	0.02
1379	1	122	ILE	HB	H	1.971	0.02
1380	1	122	ILE	HG12	H	1.916	0.02
1381	1	122	ILE	HG21	H	0.815	0.02
1382	1	122	ILE	HG22	H	0.815	0.02
1383	1	122	ILE	HG23	H	0.815	0.02
1384	1	122	ILE	HD11	H	0.883	0.02
1385	1	122	ILE	HD12	H	0.883	0.02
1386	1	122	ILE	HD13	H	0.883	0.02
1387	1	122	ILE	C	C	178.558	0.3
1388	1	122	ILE	CA	C	66.459	0.3
1389	1	122	ILE	CB	C	38.025	0.3
1390	1	122	ILE	CG1	C	30.182	0.3
1391	1	122	ILE	CG2	C	17.807	0.3
1392	1	122	ILE	CD1	C	14.322	0.3
1393	1	122	ILE	N	N	121.072	0.3
1394	1	123	ARG	H	H	8.295	0.02
1395	1	123	ARG	HA	H	3.827	0.02
1396	1	123	ARG	HB2	H	2.032	0.02
1397	1	123	ARG	HB3	H	1.847	0.02
1398	1	123	ARG	HG2	H	1.618	0.02
1399	1	123	ARG	HG3	H	1.47	0.02
1400	1	123	ARG	HD2	H	3.181	0.02
1401	1	123	ARG	HD3	H	3.032	0.02
1402	1	123	ARG	HE	H	7.7	0.02
1403	1	123	ARG	HH21	H	6.735	0.02
1404	1	123	ARG	C	C	178.267	0.3
1405	1	123	ARG	CA	C	60.377	0.3
1406	1	123	ARG	CB	C	29.731	0.3
1407	1	123	ARG	CG	C	27.338	0.3
1408	1	123	ARG	CD	C	42.937	0.3
1409	1	123	ARG	N	N	122.266	0.3
1410	1	123	ARG	NE	N	86.22	0.3
1411	1	124	VAL	H	H	7.98	0.02
1412	1	124	VAL	HA	H	3.647	0.02
1413	1	124	VAL	HB	H	2.225	0.02
1414	1	124	VAL	HG11	H	0.931	0.02
1415	1	124	VAL	HG12	H	0.931	0.02
1416	1	124	VAL	HG13	H	0.931	0.02
1417	1	124	VAL	HG21	H	1.048	0.02
1418	1	124	VAL	HG22	H	1.048	0.02
1419	1	124	VAL	HG23	H	1.048	0.02
1420	1	124	VAL	C	C	179.952	0.3
1421	1	124	VAL	CA	C	66.497	0.3
1422	1	124	VAL	CB	C	31.921	0.3
1423	1	124	VAL	CG1	C	21.242	0.3
1424	1	124	VAL	CG2	C	22.624	0.3
1425	1	124	VAL	N	N	118.405	0.3
1426	1	125	VAL	H	H	8.332	0.02
1427	1	125	VAL	HA	H	3.817	0.02
1428	1	125	VAL	HB	H	2.184	0.02
1429	1	125	VAL	HG11	H	0.886	0.02
1430	1	125	VAL	HG12	H	0.886	0.02
1431	1	125	VAL	HG13	H	0.886	0.02
1432	1	125	VAL	HG21	H	1.001	0.02
1433	1	125	VAL	HG22	H	1.001	0.02
1434	1	125	VAL	HG23	H	1.001	0.02
1435	1	125	VAL	C	C	177.933	0.3
1436	1	125	VAL	CA	C	65.931	0.3
1437	1	125	VAL	CB	C	31.861	0.3
1438	1	125	VAL	CG1	C	21.854	0.3
1439	1	125	VAL	CG2	C	22.441	0.3
1440	1	125	VAL	N	N	119.201	0.3
1441	1	126	THR	H	H	7.992	0.02
1442	1	126	THR	HA	H	4.047	0.02
1443	1	126	THR	HB	H	4.335	0.02
1444	1	126	THR	HG21	H	1.146	0.02
1445	1	126	THR	HG22	H	1.146	0.02
1446	1	126	THR	HG23	H	1.146	0.02
1447	1	126	THR	C	C	175.404	0.3
1448	1	126	THR	CA	C	63.362	0.3
1449	1	126	THR	CB	C	69.831	0.3
1450	1	126	THR	CG2	C	21.942	0.3
1451	1	126	THR	N	N	109.114	0.3
1452	1	127	GLU	H	H	7.9	0.02
1453	1	127	GLU	HA	H	4.082	0.02
1454	1	127	GLU	HB2	H	2.087	0.02
1455	1	127	GLU	HG2	H	2.329	0.02
1456	1	127	GLU	HG3	H	2.22	0.02
1457	1	127	GLU	C	C	176.38	0.3
1458	1	127	GLU	CA	C	57.541	0.3
1459	1	127	GLU	CB	C	28.768	0.3
1460	1	127	GLU	CG	C	36.625	0.3
1461	1	127	GLU	N	N	120.753	0.3
1462	1	128	ASN	H	H	8.251	0.02
1463	1	128	ASN	HA	H	4.474	0.02
1464	1	128	ASN	HB2	H	2.959	0.02
1465	1	128	ASN	HB3	H	2.863	0.02
1466	1	128	ASN	HD21	H	7.574	0.02
1467	1	128	ASN	HD22	H	6.849	0.02
1468	1	128	ASN	C	C	175.492	0.3
1469	1	128	ASN	CA	C	54.216	0.3
1470	1	128	ASN	CB	C	38.844	0.3
1471	1	128	ASN	N	N	116.329	0.3
1472	1	128	ASN	ND2	N	112.418	0.3
1473	1	129	LYS	H	H	8.12	0.02
1474	1	129	LYS	HA	H	4.245	0.02
1475	1	129	LYS	HB2	H	1.774	0.02
1476	1	129	LYS	HG2	H	1.403	0.02
1477	1	129	LYS	HD2	H	1.642	0.02
1478	1	129	LYS	HE2	H	2.956	0.02
1479	1	129	LYS	C	C	176.67	0.3
1480	1	129	LYS	CA	C	57.246	0.3
1481	1	129	LYS	CB	C	33.017	0.3
1482	1	129	LYS	CG	C	24.796	0.3
1483	1	129	LYS	CD	C	29.158	0.3
1484	1	129	LYS	CE	C	42.155	0.3
1485	1	129	LYS	N	N	120.172	0.3
1486	1	130	ILE	H	H	7.844	0.02
1487	1	130	ILE	HA	H	4.08	0.02
1488	1	130	ILE	HB	H	1.72	0.02
1489	1	130	ILE	HG12	H	1.237	0.02
1490	1	130	ILE	HG13	H	1.014	0.02
1491	1	130	ILE	HG21	H	0.689	0.02
1492	1	130	ILE	HG22	H	0.689	0.02
1493	1	130	ILE	HG23	H	0.689	0.02
1494	1	130	ILE	HD11	H	0.73	0.02
1495	1	130	ILE	HD12	H	0.73	0.02
1496	1	130	ILE	HD13	H	0.73	0.02
1497	1	130	ILE	C	C	175.414	0.3
1498	1	130	ILE	CA	C	61.409	0.3
1499	1	130	ILE	CB	C	38.642	0.3
1500	1	130	ILE	CG1	C	27.222	0.3
1501	1	130	ILE	CG2	C	17.385	0.3
1502	1	130	ILE	CD1	C	12.958	0.3
1503	1	130	ILE	N	N	118.38	0.3
1504	1	131	PHE	H	H	8.149	0.02
1505	1	131	PHE	HA	H	4.572	0.02
1506	1	131	PHE	HB2	H	3.115	0.02
1507	1	131	PHE	HB3	H	2.945	0.02
1508	1	131	PHE	C	C	175.258	0.3
1509	1	131	PHE	CA	C	57.752	0.3
1510	1	131	PHE	CB	C	39.546	0.3
1511	1	131	PHE	N	N	122.722	0.3
1512	1	132	VAL	H	H	7.761	0.02
1513	1	132	VAL	HA	H	4.023	0.02
1514	1	132	VAL	HB	H	1.97	0.02
1515	1	132	VAL	HG11	H	0.858	0.02
1516	1	132	VAL	HG12	H	0.858	0.02
1517	1	132	VAL	HG13	H	0.858	0.02
1518	1	132	VAL	C	C	175.258	0.3
1519	1	132	VAL	CA	C	62.129	0.3
1520	1	132	VAL	CB	C	33.173	0.3
1521	1	132	VAL	CG1	C	21.235	0.3
1522	1	132	VAL	N	N	121.014	0.3
1523	1	133	GLU	H	H	8.318	0.02
1524	1	133	GLU	HA	H	4.259	0.02
1525	1	133	GLU	HB2	H	1.984	0.02
1526	1	133	GLU	HB3	H	1.885	0.02
1527	1	133	GLU	HG2	H	2.211	0.02
1528	1	133	GLU	HG3	H	2.248	0.02
1529	1	133	GLU	C	C	176.08	0.3
1530	1	133	GLU	CA	C	56.352	0.3
1531	1	133	GLU	CB	C	30.57	0.3
1532	1	133	GLU	CG	C	36.244	0.3
1533	1	133	GLU	N	N	124.412	0.3
1534	1	134	VAL	H	H	8.066	0.02
1535	1	134	VAL	HA	H	4.061	0.02
1536	1	134	VAL	HB	H	2.023	0.02
1537	1	134	VAL	HG11	H	0.881	0.02
1538	1	134	VAL	HG12	H	0.881	0.02
1539	1	134	VAL	HG13	H	0.881	0.02
1540	1	134	VAL	C	C	175.727	0.3
1541	1	134	VAL	CA	C	62.125	0.3
1542	1	134	VAL	CB	C	32.993	0.3
1543	1	134	VAL	CG1	C	21.057	0.3
1544	1	134	VAL	N	N	120.725	0.3
1545	1	135	GLU	H	H	8.42	0.02
1546	1	135	GLU	HA	H	4.252	0.02
1547	1	135	GLU	HB2	H	2.014	0.02
1548	1	135	GLU	HB3	H	1.895	0.02
1549	1	135	GLU	HG2	H	2.255	0.02
1550	1	135	GLU	HG3	H	2.212	0.02
1551	1	135	GLU	C	C	175.258	0.3
1552	1	135	GLU	CA	C	56.74	0.3
1553	1	135	GLU	CB	C	30.42	0.3
1554	1	135	GLU	CG	C	36.244	0.3
1555	1	135	GLU	N	N	125.105	0.3
1556	1	136	ARG	H	H	7.925	0.02
1557	1	136	ARG	HA	H	4.133	0.02
1558	1	136	ARG	HB2	H	1.797	0.02
1559	1	136	ARG	HB3	H	1.669	0.02
1560	1	136	ARG	HG2	H	1.549	0.02
1561	1	136	ARG	HD2	H	3.139	0.02
1562	1	136	ARG	HE	H	7.176	0.02
1563	1	136	ARG	CA	C	57.419	0.3
1564	1	136	ARG	CB	C	31.664	0.3
1565	1	136	ARG	CG	C	27.141	0.3
1566	1	136	ARG	CD	C	43.557	0.3
1567	1	136	ARG	N	N	127.302	0.3

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Release date

2018-06-12

Citation

Solution structure of the N-terminal domain of proteasome lid subunit Rpn5
Zhang, W., Zhao, C., Hu, Y., Jin, C.
Biochem. Biophys. Res. Commun. (2018), 504, 225-230, PubMed 30177392 , DOI 10.1016/j.bbrc.2018.08.159 , Abstract

Related entities 1. 26S proteasome regulatory subunit RPN5, : 1 : 21 : 43 entities Detail

Interaction partners 1. 26S proteasome regulatory subunit RPN5, : 69 interactors Detail

More details for 69 interactors identified by 10 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

2 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

3 3D HNCA

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

4 3D HN(CO)CA

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

5 3D HNCO

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

6 3D HNCACB

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

7 3D HN(CA)CO

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

8 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

9 3D HCCH-COSY

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

10 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

11 3D HCCH-COSY

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

12 3D 15N-edited TOCSY-HSQC

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

13 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

14 3D 1H-15C NOESY

Instrument

Bruker AvanceIII - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	26S proteasome regulatory subunit Rpn5	[U-95% 13C; U-95% 15N]	protein	0.3 mM
2	DSS	natural abundance		0.1 mg/mL
3	DTT	natural abundance		1 mM
4	sodium phosphate	natural abundance	buffer	50 mM
5	sodium sulfate	natural abundance	salt	50 mM
6	sucrose	natural abundance		5 % w/v
7	trifluoroethanol	natural abundance		2 % v/v
8	H2O	natural abundance	solvent	90 % v/v
9	D2O	[U-99% 2H]	solvent	10 % v/v

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_36176_5zmr.nef
Input source #2: Coordindates	5zmr.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MSRDAPIKADKDYSQILKEEFPKIDSLAQNDCNSALDQLLVLEKKTRQASDLASSKEVLAKIVDLLASRNKWDDLNEQLTLLSKKHGQLKLSIQYMIQKV
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MSRDAPIKADKDYSQILKEEFPKIDSLAQNDCNSALDQLLVLEKKTRQASDLASSKEVLAKIVDLLASRNKWDDLNEQLTLLSKKHGQLKLSIQYMIQKV

-------110-------120-------130------
MEYLKSSKSLDLNTRISVIETIRVVTENKIFVEVER
||||||||||||||||||||||||||||||||||||
MEYLKSSKSLDLNTRISVIETIRVVTENKIFVEVER

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	136	0	0	100.0

Content subtype: combined_36176_5zmr.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1413		98.5 (chain: A, length: 136)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	2611 (1)	noe	97.1 (chain: A, length: 136)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 136, Sequence coverage: 98.5%
- Total number of assignments
  - ¹H chemical shifts: 712
  - ¹³C chemical shifts: 557
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
8	LYS	HZ1	7.492
8	LYS	HZ2	7.492
8	LYS	HZ3	7.492
115	ARG	HH21	6.67
123	ARG	HH21	6.735

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	866	707	81.6
¹³C chemical shifts	634	557	87.9
¹⁵N chemical shifts	155	144	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	271	266	98.2
¹³C chemical shifts	272	264	97.1
¹⁵N chemical shifts	134	131	97.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	595	441	74.1
¹³C chemical shifts	362	293	80.9
¹⁵N chemical shifts	21	13	61.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	92	77	83.7
¹³C chemical shifts	92	76	82.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	30	2	6.7
¹³C chemical shifts	29	0	0.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 136, Sequence coverage: 97.1%
- Total number of restraints: 2607
- Weight of restraints: 1.0 (2607)
- Potential type of restraints: upper-bound-parabolic (2607)
- Range of distance target values: 2.8, 5.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords HYDROLASE, a-helix bundle

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Found 1 Document (1.776 seconds)

BMRB: 36176

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. 26S proteasome regulatory subunit RPN5, : 1 : 21 : 43 entities Detail

Interaction partners 1. 26S proteasome regulatory subunit RPN5, : 69 interactors Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 3 contents Detail