Solution structure of centrin4 from Trypanosoma brucei
MAALTDEQIR EAFNLFDADG SGAIDAEEMA LAMKGLGFGD LPRDEVERTV RSMNTNANGL IEYGEFERMV KSRMAQKDSP EEVLKAFQLF DLDKKGKISF ANLKEVAKLL GENPGDDVLQ EMIAEADEDG DGEVSFEEFK SVMMQMRGK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.3 % (1365 of 1699) | 91.3 % (812 of 889) | 61.9 % (404 of 653) | 94.9 % (149 of 157) |
Backbone | 85.7 % (761 of 888) | 99.0 % (305 of 308) | 71.9 % (312 of 434) | 98.6 % (144 of 146) |
Sidechain | 78.0 % (739 of 947) | 87.3 % (507 of 581) | 64.2 % (228 of 355) | 36.4 % (4 of 11) |
Aromatic | 2.0 % (2 of 98) | 4.1 % (2 of 49) | 0.0 % (0 of 49) | |
Methyl | 77.1 % (108 of 140) | 98.6 % (69 of 70) | 55.7 % (39 of 70) |
1. Centrin, putative
MAALTDEQIR EAFNLFDADG SGAIDAEEMA LAMKGLGFGD LPRDEVERTV RSMNTNANGL IEYGEFERMV KSRMAQKDSP EEVLKAFQLF DLDKKGKISF ANLKEVAKLL GENPGDDVLQ EMIAEADEDG DGEVSFEEFK SVMMQMRGKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM [U-13C; U-15N] centrin4, 20 mM sodium phosphate, 200 mM sodium chloride, 2 mM DTT, 2 mM EDTA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | centrin4 | [U-13C; U-15N] | protein | 0.5 mM |
2 | sodium chloride | natural abundance | salt | 200 mM |
3 | sodium phosphate | natural abundance | buffer | 20 mM |
4 | DTT | natural abundance | 2 mM | |
5 | EDTA | natural abundance | 2 mM | |
6 | H2O | natural abundance | solvent | 90 % |
7 | D2O | [U-2H] | solvent | 10 % |