Solution structure of anti-CRISPR AcrIF7
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.2 % (683 of 757) | 90.3 % (346 of 383) | 88.3 % (265 of 300) | 97.3 % (72 of 74) |
Backbone | 95.1 % (390 of 410) | 93.0 % (132 of 142) | 96.0 % (193 of 201) | 97.0 % (65 of 67) |
Sidechain | 86.1 % (353 of 410) | 88.8 % (214 of 241) | 81.5 % (132 of 162) | 100.0 % (7 of 7) |
Aromatic | 66.0 % (62 of 94) | 78.7 % (37 of 47) | 52.2 % (24 of 46) | 100.0 % (1 of 1) |
Methyl | 98.5 % (65 of 66) | 100.0 % (33 of 33) | 97.0 % (32 of 33) |
1. anti-CRIPSR AcrIF7
GHMTTFTSIV TTNPDFGGFE FYVEAGQQFD DSAYEEAYGV SVPSAVVEEM NAKAAQLKDG EWLNVSHEASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8, Details 0.6 mM [U-99% 13C; U-99% 15N] AcrIF7, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AcrIF7 | [U-99% 13C; U-99% 15N] | protein | 0.6 mM |
2 | HEPES | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 150 mM |
4 | DTT | natural abundance | 2 mM | |
5 | H2O | natural abundance | solvent | 90 % |
6 | D2O | [U-2H] | solvent | 10 % |