Solution structure of recombinant APETx1
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS37:SG |
2 | disulfide | sing | 1:CYS6:SG | 1:CYS30:SG |
3 | disulfide | sing | 1:CYS20:SG | 1:CYS38:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 98.3 % (454 of 462) | 99.1 % (232 of 234) | 97.8 % (181 of 185) | 95.3 % (41 of 43) |
Backbone | 96.8 % (240 of 248) | 97.8 % (88 of 90) | 96.6 % (114 of 118) | 95.0 % (38 of 40) |
Sidechain | 100.0 % (248 of 248) | 100.0 % (144 of 144) | 100.0 % (101 of 101) | 100.0 % (3 of 3) |
Aromatic | 100.0 % (72 of 72) | 100.0 % (36 of 36) | 100.0 % (35 of 35) | 100.0 % (1 of 1) |
Methyl | 100.0 % (32 of 32) | 100.0 % (16 of 16) | 100.0 % (16 of 16) |
1. Kappa-actitoxin-Ael2a
GTTCYCGKTI GIYWFGTKTC PSNRGYTGSC GYFLGICCYP VDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.691 mM [U-100% 13C; U-100% 15N] APETx1, 20 mM potassium phosphate, 100 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | APETx1 | [U-100% 13C; U-100% 15N] | protein | 0.691 mM |
2 | potassium phosphate | natural abundance | buffer | 20 mM |
3 | sodium chloride | natural abundance | salt | 100 mM |
4 | H2O | natural abundance | solvent | 90 % |
5 | D2O | [U-2H] | solvent | 10 % |