NMR solution structures of the DNA minidumbbell formed by 5'-mCTTGXmCTTG-3'
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 91.4 % (53 of 58) | 91.4 % (53 of 58) |
Suger, PO4 | 88.1 % (37 of 42) | 88.1 % (37 of 42) |
Nucleobase | 93.8 % (15 of 16) | 93.8 % (15 of 16) |
Aromatic | 91.7 % (11 of 12) | 91.7 % (11 of 12) |
Methyl | 100.0 % (4 of 4) | 100.0 % (4 of 4) |
1. DNA (5'-D(*(MCY)P*TP*TP*GP*(3DR)P*(5CM)P*TP*TP*G)-3')
XTTGXXTTGSolvent system 99.96% D2O, Pressure 1 atm, Temperature 273 (±0.1) K, pH 7.0 (±0.1), Details 0.5 mM DNA (5'-D(*(MC1)P*TP*TP*GP*(DX)P*(DMC)P*TP*TP*G)-3'), 10 mM sodium phosphate, 0.02 mM DSS, 99.96% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*(MC1)P*TP*TP*GP*(DX)P*(DMC)P*TP*TP*G)-3') | natural abundance | DNA | 0.5 (±0.05) mM |
2 | DSS | natural abundance | 0.02 (±0.005) mM | |
3 | sodium phosphate | natural abundance | buffer | 10 (±1.0) mM |
4 | D2O | [U-2H] | solvent | 99.96 % |