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BMRB: 36378


36378
7D0X
NMR solution structures of the DNA minidumbbell formed by 5'-mCTTGXmCTTG-3'
Authors
Wan, L., Guo, P., Lam, S.L.
Assembly
DNA (5'-D(*(MCY)P*TP*TP*GP*(3DR)P*(5CM)P*TP*TP*G)-3')
Entity
1. DNA (5'-D(*(MCY)P*TP*TP*GP*(3DR)P*(5CM)P*TP*TP*G)-3') (polymer, Thiol state: not present), 9 monomers, 2599.731 Da Detail

XTTGXXTTG


2. SODIUM ION (non-polymer), 22.990 × 8 Da
Total weight
2783.651 Da
Max. entity weight
2599.731 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 66.7 %, Completeness: 91.4 %, Completeness (bb): 88.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All91.4 % (53 of 58)91.4 % (53 of 58)
Suger, PO488.1 % (37 of 42)88.1 % (37 of 42)
Nucleobase93.8 % (15 of 16)93.8 % (15 of 16)
Aromatic91.7 % (11 of 12)91.7 % (11 of 12)
Methyl100.0 % (4 of 4)100.0 % (4 of 4)

1. DNA (5'-D(*(MCY)P*TP*TP*GP*(3DR)P*(5CM)P*TP*TP*G)-3')

XTTGXXTTG

Show sample condition
Sample

Solvent system 99.96% D2O, Pressure 1 atm, Temperature 273 (±0.1) K, pH 7.0 (±0.1), Details 0.5 mM DNA (5'-D(*(MC1)P*TP*TP*GP*(DX)P*(DMC)P*TP*TP*G)-3'), 10 mM sodium phosphate, 0.02 mM DSS, 99.96% D2O


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*(MC1)P*TP*TP*GP*(DX)P*(DMC)P*TP*TP*G)-3')natural abundanceDNA0.5 (±0.05) mM
2DSSnatural abundance0.02 (±0.005) mM
3sodium phosphatenatural abundancebuffer10 (±1.0) mM
4D2O[U-2H]solvent99.96 %

Release date
2021-02-09
Citation
5-Methylcytosine Substantially Enhances the Thermal Stability of DNA Minidumbbells
Wan, L., Yi, J., Lam, S.L., Lee, H.K., Guo, P.
Chemistry (2021), 27, 6740-6747, PubMed 33501691 , DOI 10.1002/chem.202005410 ,
Experiments performed 3 experiments Detail
nullKeywords 5-methylcytosine, DNA, abasic site, minidumbbell