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BMRB: 36528

8HQB

NMR Structure of OsCIE1-Ubox

Authors

Zhang, Y., Yu, C.Z., Lan, W.X.

Assembly

U-box domain-containing protein 12 (E.C.2.3.2.27)

Entity

1. U-box domain-containing protein 12 (E.C.2.3.2.27) (polymer, Thiol state: all free), 80 monomers, 8944.205 × 2 Da Detail

GAMIIPDEFR CPISLELMQD PVIVSSGQTY ERSCIQKWLD SGHKTCPKTQ QPLSHTSLTP NFVLKSLISQ WCEANGIELP

Total weight

17888.41 Da

Max. entity weight

8944.205 Da

Source organism

Oryza sativa Japonica Group

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chemcal Shifts

¹H: 539, ¹³C: 305, ¹⁵N: 72 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Ambig. code
1	1	2	ALA	CA	C	52.413	1
2	1	2	ALA	CB	C	19.554	1
3	1	2	ALA	HA	H	4.362	1
4	1	2	ALA	HB1	H	1.388	1
5	1	2	ALA	HB2	H	1.388	1
6	1	2	ALA	HB3	H	1.388	1
7	1	3	MET	CA	C	55.178	1
8	1	3	MET	CB	C	33.263	1
9	1	3	MET	C	C	175.491	1
10	1	3	MET	HA	H	4.508	1
11	1	3	MET	HB3	H	1.589
12	1	3	MET	HB2	H	2.074
13	1	3	MET	HE1	H	1.47	1
14	1	3	MET	HE2	H	1.47	1
15	1	3	MET	HE3	H	1.47	1
16	1	3	MET	HG3	H	2.595
17	1	3	MET	HG2	H	2.595
18	1	3	MET	H	H	8.426	1
19	1	3	MET	N	N	120.405	1
20	1	4	ILE	CA	C	60.539	1
21	1	4	ILE	CB	C	38.372	1
22	1	4	ILE	CD1	C	12.41	1
23	1	4	ILE	CG1	C	27.327	1
24	1	4	ILE	CG2	C	17.374	1
25	1	4	ILE	C	C	175.527	1
26	1	4	ILE	HA	H	4.176	1
27	1	4	ILE	HB	H	1.838	1
28	1	4	ILE	HD11	H	0.824	1
29	1	4	ILE	HD12	H	0.824	1
30	1	4	ILE	HD13	H	0.824	1
31	1	4	ILE	HG13	H	1.191
32	1	4	ILE	HG12	H	1.54
33	1	4	ILE	HG21	H	0.805	1
34	1	4	ILE	HG22	H	0.805	1
35	1	4	ILE	HG23	H	0.805	1
36	1	4	ILE	H	H	8.384	1
37	1	4	ILE	N	N	125.134	1
38	1	5	ILE	CA	C	58.45	1
39	1	5	ILE	CB	C	39.626	1
40	1	5	ILE	CD1	C	13.93	1
41	1	5	ILE	CG1	C	27.374	1
42	1	5	ILE	CG2	C	17.483	1
43	1	5	ILE	HA	H	4.152	1
44	1	5	ILE	HB	H	1.554	1
45	1	5	ILE	HD11	H	0.907	1
46	1	5	ILE	HD12	H	0.907	1
47	1	5	ILE	HD13	H	0.907	1
48	1	5	ILE	HG13	H	1.192
49	1	5	ILE	HG12	H	1.192
50	1	5	ILE	HG21	H	0.653	1
51	1	5	ILE	HG22	H	0.653	1
52	1	5	ILE	HG23	H	0.653	1
53	1	5	ILE	H	H	8.237	1
54	1	5	ILE	N	N	127.538	1
55	1	6	PRO	CA	C	62.816	1
56	1	6	PRO	CB	C	31.374	1
57	1	6	PRO	CD	C	50.874	1
58	1	6	PRO	C	C	177.26	1
59	1	6	PRO	HA	H	3.99	1
60	1	6	PRO	HB2	H	1.776
61	1	6	PRO	HB3	H	1.776
62	1	6	PRO	HD2	H	3.462
63	1	6	PRO	HD3	H	3.462
64	1	6	PRO	HG2	H	2.118
65	1	6	PRO	HG3	H	2.118
66	1	7	ASP	CA	C	58.125	1
67	1	7	ASP	CB	C	40.733	1
68	1	7	ASP	C	C	178.408	1
69	1	7	ASP	HA	H	3.931	1
70	1	7	ASP	HB2	H	2.383
71	1	7	ASP	HB3	H	2.383
72	1	7	ASP	H	H	8.597	1
73	1	7	ASP	N	N	125.278	1
74	1	8	GLU	CA	C	60.154	1
75	1	8	GLU	CB	C	28.573	1
76	1	8	GLU	CG	C	32.124	1
77	1	8	GLU	C	C	176.86	1
78	1	8	GLU	HA	H	3.814	1
79	1	8	GLU	HB2	H	1.69
80	1	8	GLU	HB3	H	1.69
81	1	8	GLU	HG3	H	1.903
82	1	8	GLU	HG2	H	2.21
83	1	8	GLU	H	H	9.194	1
84	1	8	GLU	N	N	116.21	1
85	1	9	PHE	CA	C	53.196	1
86	1	9	PHE	CB	C	37.178	1
87	1	9	PHE	CD1	C	123.72
88	1	9	PHE	CD2	C	123.72
89	1	9	PHE	CE1	C	126.088
90	1	9	PHE	CE2	C	126.088
91	1	9	PHE	C	C	174.539	1
92	1	9	PHE	CZ	C	123.692	1
93	1	9	PHE	HA	H	4.61	1
94	1	9	PHE	HB3	H	2.684
95	1	9	PHE	HB2	H	3.274
96	1	9	PHE	HD1	H	6.292
97	1	9	PHE	HD2	H	6.292
98	1	9	PHE	HE1	H	6.843
99	1	9	PHE	HE2	H	6.843
100	1	9	PHE	H	H	7.406	1
101	1	9	PHE	HZ	H	7.004	1
102	1	9	PHE	N	N	114.225	1
103	1	10	ARG	CA	C	54.541	1
104	1	10	ARG	CB	C	32.529	1
105	1	10	ARG	CG	C	32.04	1
106	1	10	ARG	CD	C	43.684	1
107	1	10	ARG	C	C	175.383	1
108	1	10	ARG	HA	H	4.508	1
109	1	10	ARG	HB2	H	1.582
110	1	10	ARG	HB3	H	1.582
111	1	10	ARG	HD2	H	3.024
112	1	10	ARG	HD3	H	3.024
113	1	10	ARG	HG2	H	1.046
114	1	10	ARG	HG3	H	1.046
115	1	10	ARG	H	H	7.457	1
116	1	10	ARG	N	N	118.655	1
117	1	11	CYS	CA	C	59.073	1
118	1	11	CYS	CB	C	30.305	1
119	1	11	CYS	HA	H	4.584	1
120	1	11	CYS	HB3	H	2.807
121	1	11	CYS	HB2	H	3.503
122	1	11	CYS	H	H	8.496	1
123	1	11	CYS	N	N	131.49	1
124	1	12	PRO	CA	C	64.457	1
125	1	12	PRO	CB	C	33.237	1
126	1	12	PRO	CD	C	52.598	1
127	1	12	PRO	C	C	177.84	1
128	1	12	PRO	HA	H	4.336	1
129	1	12	PRO	HB2	H	1.761
130	1	12	PRO	HB3	H	1.761
131	1	12	PRO	HD3	H	4.01
132	1	12	PRO	HD2	H	4.121
133	1	12	PRO	HG2	H	2.362
134	1	12	PRO	HG3	H	2.362
135	1	13	ILE	CA	C	63.897	1
136	1	13	ILE	CB	C	38.69	1
137	1	13	ILE	CD1	C	13.753	1
138	1	13	ILE	CG1	C	26.769	1
139	1	13	ILE	CG2	C	17.34	1
140	1	13	ILE	C	C	177.61	1
141	1	13	ILE	HA	H	3.925	1
142	1	13	ILE	HB	H	2.233	1
143	1	13	ILE	HD11	H	0.251	1
144	1	13	ILE	HD12	H	0.251	1
145	1	13	ILE	HD13	H	0.251	1
146	1	13	ILE	HG13	H	1.554
147	1	13	ILE	HG12	H	1.554
148	1	13	ILE	HG21	H	0.842	1
149	1	13	ILE	HG22	H	0.842	1
150	1	13	ILE	HG23	H	0.842	1
151	1	13	ILE	H	H	11.166	1
152	1	13	ILE	N	N	122.328	1
153	1	14	SER	CA	C	59.583	1
154	1	14	SER	CB	C	64.483	1
155	1	14	SER	C	C	175.621	1
156	1	14	SER	HA	H	4.168	1
157	1	14	SER	HB2	H	4.036
158	1	14	SER	HB3	H	4.036
159	1	14	SER	H	H	9.498	1
160	1	14	SER	N	N	118.103	1
161	1	15	LEU	CA	C	57.294	1
162	1	15	LEU	CB	C	39.841	1
163	1	15	LEU	CD1	C	23
164	1	15	LEU	CD2	C	22.9106
165	1	15	LEU	C	C	175.821	1
166	1	15	LEU	HA	H	4.009	1
167	1	15	LEU	HB2	H	1.616
168	1	15	LEU	HB3	H	2.17
169	1	15	LEU	HD11	H	0.807
170	1	15	LEU	HD12	H	0.807
171	1	15	LEU	HD13	H	0.807
172	1	15	LEU	HD21	H	1.102
173	1	15	LEU	HD22	H	1.102
174	1	15	LEU	HD23	H	1.102
175	1	15	LEU	HG	H	1.467	1
176	1	15	LEU	H	H	8.793	1
177	1	15	LEU	N	N	117.958	1
178	1	16	GLU	CA	C	54.428	1
179	1	16	GLU	CB	C	32.251	1
180	1	16	GLU	C	C	176.012	1
181	1	16	GLU	HA	H	4.513	1
182	1	16	GLU	HB2	H	1.738
183	1	16	GLU	HB3	H	1.738
184	1	16	GLU	HG2	H	2.083
185	1	16	GLU	HG3	H	2.083
186	1	16	GLU	H	H	8.003	1
187	1	16	GLU	N	N	118.825	1
188	1	17	LEU	CA	C	55.564	1
189	1	17	LEU	CB	C	43.024	1
190	1	17	LEU	CD1	C	23.016
191	1	17	LEU	CD2	C	23.558
192	1	17	LEU	C	C	177.196	1
193	1	17	LEU	HA	H	4.035	1
194	1	17	LEU	HB3	H	1.436
195	1	17	LEU	HB2	H	1.656
196	1	17	LEU	HD11	H	0.664
197	1	17	LEU	HD12	H	0.664
198	1	17	LEU	HD13	H	0.664
199	1	17	LEU	HD21	H	0.907
200	1	17	LEU	HD22	H	0.907
201	1	17	LEU	HD23	H	0.907
202	1	17	LEU	HG	H	1.45	1
203	1	17	LEU	H	H	8.582	1
204	1	17	LEU	N	N	122.33	1
205	1	18	MET	CA	C	57.483	1
206	1	18	MET	CB	C	35.205	1
207	1	18	MET	CG	C	34.454	1
208	1	18	MET	CE	C	17.305	1
209	1	18	MET	C	C	174.251	1
210	1	18	MET	HA	H	4.277	1
211	1	18	MET	HB3	H	1.299
212	1	18	MET	HB2	H	1.89
213	1	18	MET	HE1	H	1.656	1
214	1	18	MET	HE2	H	1.656	1
215	1	18	MET	HE3	H	1.656	1
216	1	18	MET	HG3	H	2.317
217	1	18	MET	HG2	H	2.675
218	1	18	MET	H	H	8.315	1
219	1	18	MET	N	N	126.527	1
220	1	19	GLN	CA	C	56.761	1
221	1	19	GLN	CB	C	30.509	1
222	1	19	GLN	CG	C	33.69	1
223	1	19	GLN	C	C	176.988	1
224	1	19	GLN	HA	H	4.34	1
225	1	19	GLN	HB2	H	2.082
226	1	19	GLN	HB3	H	2.082
227	1	19	GLN	HG2	H	2.379
228	1	19	GLN	HG3	H	2.379
229	1	19	GLN	H	H	10.065	1
230	1	19	GLN	N	N	122.242	1
231	1	20	ASP	CA	C	50.975	1
232	1	20	ASP	CB	C	40.685	1
233	1	20	ASP	HA	H	5	1
234	1	20	ASP	HB3	H	2.355
235	1	20	ASP	HB2	H	3.099
236	1	20	ASP	H	H	10.343	1
237	1	20	ASP	N	N	122.836	1
238	1	21	PRO	CA	C	62.038	1
239	1	21	PRO	CB	C	33.494	1
240	1	21	PRO	CD	C	49.875	1
241	1	21	PRO	C	C	178.027	1
242	1	21	PRO	HA	H	4.231	1
243	1	21	PRO	HB2	H	2.25
244	1	21	PRO	HB3	H	2.25
245	1	21	PRO	HD3	H	3.586
246	1	21	PRO	HD2	H	3.78
247	1	21	PRO	HG2	H	1.936
248	1	21	PRO	HG3	H	1.936
249	1	22	VAL	CA	C	58.294	1
250	1	22	VAL	CB	C	35.443	1
251	1	22	VAL	C	C	172.496	1
252	1	22	VAL	HA	H	5.037	1
253	1	22	VAL	HB	H	1.74	1
254	1	22	VAL	HG11	H	0.686
255	1	22	VAL	HG12	H	0.686
256	1	22	VAL	HG13	H	0.686
257	1	22	VAL	HG21	H	0.921
258	1	22	VAL	HG22	H	0.921
259	1	22	VAL	HG23	H	0.921
260	1	22	VAL	H	H	9.873	1
261	1	22	VAL	N	N	120.002	1
262	1	22	VAL	CG1	C	21.005
263	1	22	VAL	CG2	C	20.466
264	1	23	ILE	CA	C	58.582	1
265	1	23	ILE	CB	C	42.454	1
266	1	23	ILE	CD1	C	13.392	1
267	1	23	ILE	CG1	C	14.845	1
268	1	23	ILE	CG2	C	15.725	1
269	1	23	ILE	C	C	176.599	1
270	1	23	ILE	HA	H	5.346	1
271	1	23	ILE	HB	H	1.511	1
272	1	23	ILE	HD11	H	0.886	1
273	1	23	ILE	HD12	H	0.886	1
274	1	23	ILE	HD13	H	0.886	1
275	1	23	ILE	HG12	H	0.982
276	1	23	ILE	HG13	H	0.982
277	1	23	ILE	HG21	H	0.788	1
278	1	23	ILE	HG22	H	0.788	1
279	1	23	ILE	HG23	H	0.788	1
280	1	23	ILE	H	H	9.714	1
281	1	23	ILE	N	N	123.163	1
282	1	24	VAL	CA	C	59.57	1
283	1	24	VAL	CB	C	33.989	1
284	1	24	VAL	CG1	C	23.29
285	1	24	VAL	CG2	C	23.29
286	1	24	VAL	C	C	178.601	1
287	1	24	VAL	HA	H	4.948	1
288	1	24	VAL	HB	H	1.345	1
289	1	24	VAL	H	H	7.843	1
290	1	24	VAL	N	N	117.563	1
291	1	24	VAL	HG11	H	0.996
292	1	24	VAL	HG12	H	0.996
293	1	24	VAL	HG13	H	0.996
294	1	24	VAL	HG21	H	0.996
295	1	24	VAL	HG22	H	0.996
296	1	24	VAL	HG23	H	0.996
297	1	25	SER	CA	C	62.733	1
298	1	25	SER	CB	C	63.041	1
299	1	25	SER	C	C	174.549	1
300	1	25	SER	HB2	H	4.086
301	1	25	SER	HB3	H	4.086
302	1	25	SER	H	H	9.398	1
303	1	25	SER	N	N	116.505	1
304	1	26	SER	CA	C	58.938	1
305	1	26	SER	CB	C	64.432	1
306	1	26	SER	C	C	176.121	1
307	1	26	SER	HA	H	4.474	1
308	1	26	SER	HB3	H	3.722
309	1	26	SER	HB2	H	4.28
310	1	26	SER	H	H	6.905	1
311	1	26	SER	N	N	114.514	1
312	1	27	GLY	CA	C	45.514	1
313	1	27	GLY	C	C	173.587	1
314	1	27	GLY	HA3	H	3.781
315	1	27	GLY	HA2	H	4.407
316	1	27	GLY	H	H	9.03	1
317	1	27	GLY	N	N	111.086	1
318	1	28	GLN	CA	C	54.333	1
319	1	28	GLN	CB	C	29.672	1
320	1	28	GLN	CG	C	34.318	1
321	1	28	GLN	C	C	173.47	1
322	1	28	GLN	HA	H	4.306	1
323	1	28	GLN	HB2	H	1.763
324	1	28	GLN	HB3	H	1.763
325	1	28	GLN	HE21	H	6.567
326	1	28	GLN	HE22	H	7.436
327	1	28	GLN	HG3	H	2.048
328	1	28	GLN	HG2	H	2.235
329	1	28	GLN	H	H	7.222	1
330	1	28	GLN	N	N	118.126	1
331	1	28	GLN	NE2	N	115.035	1
332	1	29	THR	CA	C	62.201	1
333	1	29	THR	CB	C	69.648	1
334	1	29	THR	CG2	C	22.998	1
335	1	29	THR	C	C	172.315	1
336	1	29	THR	HA	H	5.341	1
337	1	29	THR	HB	H	3.688	1
338	1	29	THR	HG21	H	1.079	1
339	1	29	THR	HG22	H	1.079	1
340	1	29	THR	HG23	H	1.079	1
341	1	29	THR	H	H	7.097	1
342	1	29	THR	N	N	118.355	1
343	1	30	TYR	CA	C	56.583	1
344	1	30	TYR	CB	C	45.428	1
345	1	30	TYR	CE1	C	111.565
346	1	30	TYR	CE2	C	111.565
347	1	30	TYR	C	C	175.413	1
348	1	30	TYR	HA	H	5.162	1
349	1	30	TYR	HB3	H	2.112
350	1	30	TYR	HB2	H	3.302
351	1	30	TYR	HD1	H	7.082
352	1	30	TYR	HD2	H	7.082
353	1	30	TYR	HE1	H	6.543
354	1	30	TYR	HE2	H	6.543
355	1	30	TYR	HH	H	9.478	1
356	1	30	TYR	H	H	9.203	1
357	1	30	TYR	N	N	121.536	1
358	1	31	GLU	CA	C	56.825	1
359	1	31	GLU	CB	C	30.256	1
360	1	31	GLU	CG	C	33.084	1
361	1	31	GLU	C	C	179.237	1
362	1	31	GLU	HA	H	4.403	1
363	1	31	GLU	HB3	H	2.049
364	1	31	GLU	HB2	H	2.166
365	1	31	GLU	HG2	H	2.314
366	1	31	GLU	HG3	H	2.314
367	1	31	GLU	H	H	10.447	1
368	1	31	GLU	N	N	124.435	1
369	1	32	ARG	CA	C	61.28	1
370	1	32	ARG	CB	C	30.093	1
371	1	32	ARG	C	C	177.685	1
372	1	32	ARG	HA	H	3.803	1
373	1	32	ARG	HB2	H	1.932
374	1	32	ARG	HB3	H	1.932
375	1	32	ARG	HD3	H	2.939
376	1	32	ARG	HD2	H	3.104
377	1	32	ARG	HG2	H	1.364
378	1	32	ARG	HG3	H	1.364
379	1	32	ARG	H	H	8.581	1
380	1	32	ARG	N	N	131.15	1
381	1	33	SER	CA	C	61.645	1
382	1	33	SER	CB	C	62.272	1
383	1	33	SER	C	C	177.24	1
384	1	33	SER	HA	H	4.146	1
385	1	33	SER	HB2	H	3.992
386	1	33	SER	HB3	H	3.992
387	1	33	SER	H	H	9.423	1
388	1	33	SER	N	N	111.842	1
389	1	34	CYS	CA	C	60.206	1
390	1	34	CYS	CB	C	28.062	1
391	1	34	CYS	C	C	178.057	1
392	1	34	CYS	HA	H	4.222	1
393	1	34	CYS	HB3	H	2.92
394	1	34	CYS	HB2	H	3.2
395	1	34	CYS	H	H	7.132	1
396	1	34	CYS	N	N	119.157	1
397	1	35	ILE	CA	C	59.259	1
398	1	35	ILE	CB	C	38.12	1
399	1	35	ILE	CD1	C	13.812	1
400	1	35	ILE	CG2	C	21.881	1
401	1	35	ILE	C	C	176.024	1
402	1	35	ILE	HA	H	4.203	1
403	1	35	ILE	HB	H	1.472	1
404	1	35	ILE	HD11	H	0.197	1
405	1	35	ILE	HD12	H	0.197	1
406	1	35	ILE	HD13	H	0.197	1
407	1	35	ILE	HG13	H	0.994
408	1	35	ILE	HG12	H	1.193
409	1	35	ILE	HG21	H	0.703	1
410	1	35	ILE	HG22	H	0.703	1
411	1	35	ILE	HG23	H	0.703	1
412	1	35	ILE	H	H	8.02	1
413	1	35	ILE	N	N	119.391	1
414	1	36	GLN	CA	C	58.954	1
415	1	36	GLN	CB	C	28.981	1
416	1	36	GLN	CG	C	33.66	1
417	1	36	GLN	C	C	177.707	1
418	1	36	GLN	HA	H	4.029	1
419	1	36	GLN	HB2	H	2.079
420	1	36	GLN	HB3	H	2.079
421	1	36	GLN	HE21	H	6.784
422	1	36	GLN	HE22	H	7.917
423	1	36	GLN	HG2	H	2.377
424	1	36	GLN	HG3	H	2.377
425	1	36	GLN	H	H	9.012	1
426	1	36	GLN	N	N	125.02	1
427	1	36	GLN	NE2	N	115.507	1
428	1	37	LYS	CA	C	59.716	1
429	1	37	LYS	CB	C	32.394	1
430	1	37	LYS	CD	C	29.04	1
431	1	37	LYS	CG	C	25.235	1
432	1	37	LYS	CE	C	42.212	1
433	1	37	LYS	C	C	179.158	1
434	1	37	LYS	HA	H	4.183	1
435	1	37	LYS	HB2	H	2.019
436	1	37	LYS	HB3	H	2.019
437	1	37	LYS	HD2	H	1.71
438	1	37	LYS	HD3	H	1.71
439	1	37	LYS	HE3	H	2.878
440	1	37	LYS	HE2	H	3.084
441	1	37	LYS	HG2	H	1.56
442	1	37	LYS	HG3	H	1.56
443	1	37	LYS	H	H	7.561	1
444	1	37	LYS	N	N	118.035	1
445	1	38	TRP	CA	C	60.186	1
446	1	38	TRP	CB	C	29.871	1
447	1	38	TRP	CD1	C	120.917	1
448	1	38	TRP	CE3	C	115.334	1
449	1	38	TRP	CH2	C	119.701	1
450	1	38	TRP	C	C	178.29	1
451	1	38	TRP	CZ2	C	109.113	1
452	1	38	TRP	CZ3	C	109.659	1
453	1	38	TRP	HA	H	4.472	1
454	1	38	TRP	HB3	H	3.22
455	1	38	TRP	HB2	H	3.662
456	1	38	TRP	HD1	H	7.071	1
457	1	38	TRP	HE1	H	10.072	1
458	1	38	TRP	HE3	H	7.25	1
459	1	38	TRP	HH2	H	6.773	1
460	1	38	TRP	H	H	7.418	1
461	1	38	TRP	HZ2	H	6.991	1
462	1	38	TRP	HZ3	H	7.442	1
463	1	38	TRP	N	N	121.897	1
464	1	38	TRP	NE1	N	130.118	1
465	1	39	LEU	CA	C	57.602	1
466	1	39	LEU	CB	C	41.292	1
467	1	39	LEU	CD1	C	22.53
468	1	39	LEU	CD2	C	22.53
469	1	39	LEU	C	C	181.944	1
470	1	39	LEU	HA	H	4.39	1
471	1	39	LEU	HB3	H	1.56
472	1	39	LEU	HB2	H	2.034
473	1	39	LEU	HD11	H	0.927
474	1	39	LEU	HD12	H	0.927
475	1	39	LEU	HD13	H	0.927
476	1	39	LEU	HD21	H	0.927
477	1	39	LEU	HD22	H	0.927
478	1	39	LEU	HD23	H	0.927
479	1	39	LEU	HG	H	1.562	1
480	1	39	LEU	H	H	8.394	1
481	1	39	LEU	N	N	119.83	1
482	1	40	ASP	CA	C	56.957	1
483	1	40	ASP	CB	C	40.098	1
484	1	40	ASP	C	C	177.785	1
485	1	40	ASP	HA	H	4.411	1
486	1	40	ASP	HB3	H	2.707
487	1	40	ASP	HB2	H	2.849
488	1	40	ASP	H	H	9.274	1
489	1	40	ASP	N	N	122.167	1
490	1	41	SER	CA	C	58.729	1
491	1	41	SER	CB	C	63.301	1
492	1	41	SER	C	C	173.965	1
493	1	41	SER	HA	H	4.407	1
494	1	41	SER	HB2	H	3.971
495	1	41	SER	HB3	H	3.971
496	1	41	SER	H	H	7.636	1
497	1	41	SER	N	N	113.513	1
498	1	42	GLY	CA	C	44.951	1
499	1	42	GLY	C	C	173.741	1
500	1	42	GLY	HA3	H	3.525
501	1	42	GLY	HA2	H	4.026
502	1	42	GLY	H	H	7.767	1
503	1	42	GLY	N	N	107.609	1
504	1	43	HIS	CA	C	56.065	1
505	1	43	HIS	CB	C	29.261	1
506	1	43	HIS	CD2	C	115.388	1
507	1	43	HIS	HA	H	4.206	1
508	1	43	HIS	HB3	H	1.254
509	1	43	HIS	HB2	H	2.269
510	1	43	HIS	HD2	H	6.071	1
511	1	43	HIS	H	H	6.68	1
512	1	43	HIS	N	N	117.567	1
513	1	44	LYS	CA	C	54.802	1
514	1	44	LYS	CB	C	32.754	1
515	1	44	LYS	CG	C	25.139	1
516	1	44	LYS	CE	C	42.197	1
517	1	44	LYS	C	C	175.706	1
518	1	44	LYS	HA	H	4.518	1
519	1	44	LYS	HB3	H	1.6
520	1	44	LYS	HB2	H	2.083
521	1	44	LYS	HG2	H	1.293
522	1	44	LYS	HG3	H	1.293
523	1	44	LYS	HD2	H	1.585
524	1	44	LYS	HD3	H	1.585
525	1	44	LYS	HE2	H	2.891
526	1	44	LYS	HE3	H	2.891
527	1	45	THR	CA	C	58.582	1
528	1	45	THR	CB	C	72.047	1
529	1	45	THR	CG2	C	22.331	1
530	1	45	THR	C	C	172.572	1
531	1	45	THR	HA	H	4.813	1
532	1	45	THR	HB	H	3.73	1
533	1	45	THR	HG21	H	0.99	1
534	1	45	THR	HG22	H	0.99	1
535	1	45	THR	HG23	H	0.99	1
536	1	45	THR	H	H	7.132	1
537	1	45	THR	N	N	107.83	1
538	1	46	CYS	CA	C	57.045	1
539	1	46	CYS	CB	C	29.162	1
540	1	46	CYS	HA	H	4.32	1
541	1	46	CYS	HB2	H	2.864
542	1	46	CYS	HB3	H	2.864
543	1	46	CYS	H	H	8.558	1
544	1	46	CYS	N	N	124.545	1
545	1	47	PRO	CA	C	64.976	1
546	1	47	PRO	CB	C	30.853	1
547	1	47	PRO	CD	C	49.979	1
548	1	47	PRO	CG	C	25.715	1
549	1	47	PRO	C	C	177.393	1
550	1	47	PRO	HA	H	4.3	1
551	1	47	PRO	HB3	H	0.842
552	1	47	PRO	HB2	H	1.235
553	1	47	PRO	HD3	H	3.294
554	1	47	PRO	HD2	H	3.578
555	1	47	PRO	HG2	H	-0.204
556	1	47	PRO	HG3	H	-0.204
557	1	48	LYS	CA	C	58.531	1
558	1	48	LYS	CB	C	34.486	1
559	1	48	LYS	CE	C	42.2	1
560	1	48	LYS	CG	C	25.139	1
561	1	48	LYS	CD	C	29.053	1
562	1	48	LYS	C	C	177.817	1
563	1	48	LYS	HA	H	4.15	1
564	1	48	LYS	HB2	H	1.734
565	1	48	LYS	HB3	H	1.734
566	1	48	LYS	HD2	H	1.601
567	1	48	LYS	HD3	H	1.601
568	1	48	LYS	HE2	H	2.833
569	1	48	LYS	HE3	H	2.833
570	1	48	LYS	HG2	H	1.279
571	1	48	LYS	HG3	H	1.279
572	1	48	LYS	H	H	8.086	1
573	1	48	LYS	N	N	116.455	1
574	1	49	THR	CA	C	62.618	1
575	1	49	THR	CB	C	72.123	1
576	1	49	THR	CG2	C	21.319	1
577	1	49	THR	C	C	175.608	1
578	1	49	THR	HA	H	4.398	1
579	1	49	THR	HB	H	4.414	1
580	1	49	THR	HG21	H	1.202	1
581	1	49	THR	HG22	H	1.202	1
582	1	49	THR	HG23	H	1.202	1
583	1	49	THR	H	H	8.29	1
584	1	49	THR	N	N	108.987	1
585	1	50	GLN	CA	C	57.446	1
586	1	50	GLN	CB	C	26.265	1
587	1	50	GLN	CG	C	33.64	1
588	1	50	GLN	C	C	174.44	1
589	1	50	GLN	HA	H	3.909	1
590	1	50	GLN	HB2	H	2.327
591	1	50	GLN	HB3	H	2.327
592	1	50	GLN	HG2	H	2.352
593	1	50	GLN	HG3	H	2.352
594	1	50	GLN	H	H	8.618	1
595	1	50	GLN	N	N	116.368	1
596	1	51	GLN	CA	C	53.742	1
597	1	51	GLN	CB	C	29.785	1
598	1	51	GLN	CG	C	33.792	1
599	1	51	GLN	HA	H	4.603	1
600	1	51	GLN	HB2	H	1.757
601	1	51	GLN	HB3	H	1.757
602	1	51	GLN	HE21	H	6.246
603	1	51	GLN	HG3	H	2.015
604	1	51	GLN	HG2	H	2.389
605	1	51	GLN	H	H	7.332	1
606	1	51	GLN	N	N	117.889	1
607	1	52	PRO	CA	C	63.222	1
608	1	52	PRO	CB	C	31.969	1
609	1	52	PRO	CD	C	50.4	1
610	1	52	PRO	C	C	177.145	1
611	1	52	PRO	HA	H	4.368	1
612	1	52	PRO	HB3	H	1.806
613	1	52	PRO	HB2	H	2.317
614	1	52	PRO	HD3	H	3.663
615	1	52	PRO	HD2	H	3.91
616	1	52	PRO	HG2	H	2.074
617	1	52	PRO	HG3	H	2.074
618	1	53	LEU	CA	C	54.006	1
619	1	53	LEU	CB	C	40.967	1
620	1	53	LEU	CD2	C	22.899
621	1	53	LEU	CD1	C	22.776
622	1	53	LEU	HA	H	4.359	1
623	1	53	LEU	HB3	H	1.39
624	1	53	LEU	HB2	H	1.39
625	1	53	LEU	HD11	H	0.781
626	1	53	LEU	HD12	H	0.781
627	1	53	LEU	HD13	H	0.781
628	1	53	LEU	HD21	H	0.978
629	1	53	LEU	HD22	H	0.978
630	1	53	LEU	HD23	H	0.978
631	1	53	LEU	HG	H	1.724	1
632	1	53	LEU	H	H	8.274	1
633	1	53	LEU	N	N	123.913	1
634	1	54	SER	CA	C	59.889	1
635	1	54	SER	CB	C	62.94	1
636	1	54	SER	C	C	174.061	1
637	1	54	SER	HA	H	4.214	1
638	1	54	SER	HB2	H	3.913
639	1	54	SER	HB3	H	3.913
640	1	55	HIS	CA	C	55.311	1
641	1	55	HIS	CB	C	32.375	1
642	1	55	HIS	CD2	C	113.418	1
643	1	55	HIS	C	C	176.424	1
644	1	55	HIS	HA	H	4.75	1
645	1	55	HIS	HB2	H	3.2
646	1	55	HIS	HB3	H	3.2
647	1	55	HIS	HD2	H	6.882	1
648	1	55	HIS	H	H	7.55	1
649	1	55	HIS	N	N	115.889	1
650	1	56	THR	HG21	H	1.335	1
651	1	56	THR	HG22	H	1.335	1
652	1	56	THR	HG23	H	1.335	1
653	1	56	THR	H	H	7.878	1
654	1	56	THR	N	N	120.89	1
655	1	58	LEU	CA	C	54.005	1
656	1	58	LEU	C	C	176.463	1
657	1	59	THR	CA	C	59.747	1
658	1	59	THR	CB	C	71.465	1
659	1	59	THR	CG2	C	21.034	1
660	1	59	THR	HB	H	3.866	1
661	1	59	THR	HG21	H	1.262	1
662	1	59	THR	HG22	H	1.262	1
663	1	59	THR	HG23	H	1.262	1
664	1	59	THR	H	H	7.882	1
665	1	59	THR	N	N	120.899	1
666	1	60	PRO	CA	C	62.699	1
667	1	60	PRO	HA	H	4.268	1
668	1	60	PRO	HB2	H	1.93
669	1	60	PRO	HB3	H	1.93
670	1	60	PRO	HG2	H	2.201
671	1	60	PRO	HG3	H	2.201
672	1	61	ASN	CA	C	54.494	1
673	1	61	ASN	C	C	176	1
674	1	61	ASN	HA	H	4.754	1
675	1	61	ASN	HB2	H	2.986
676	1	61	ASN	HB3	H	2.986
677	1	61	ASN	H	H	8.181	1
678	1	61	ASN	N	N	119.886	1
679	1	62	PHE	CE1	C	126.887
680	1	62	PHE	CE2	C	126.887
681	1	62	PHE	CA	C	62.504	1
682	1	62	PHE	CB	C	38.406	1
683	1	62	PHE	CD1	C	126.166
684	1	62	PHE	CD2	C	126.166
685	1	62	PHE	CZ	C	133.798	1
686	1	62	PHE	HA	H	4.233	1
687	1	62	PHE	HB3	H	2.898
688	1	62	PHE	HB2	H	3.147
689	1	62	PHE	HD1	H	7.28
690	1	62	PHE	HD2	H	7.28
691	1	62	PHE	HE1	H	7.521
692	1	62	PHE	HE2	H	7.521
693	1	62	PHE	H	H	7.394	1
694	1	62	PHE	HZ	H	7.542	1
695	1	62	PHE	N	N	119.549	1
696	1	63	VAL	C	C	176.353	1
697	1	63	VAL	CG1	C	21.51
698	1	63	VAL	CG2	C	21.51
699	1	63	VAL	HA	H	4.154	1
700	1	63	VAL	HB	H	2.17	1
701	1	63	VAL	HG11	H	1.086
702	1	63	VAL	HG12	H	1.086
703	1	63	VAL	HG13	H	1.086
704	1	63	VAL	HG21	H	1.086
705	1	63	VAL	HG22	H	1.086
706	1	63	VAL	HG23	H	1.086
707	1	63	VAL	H	H	9.038	1
708	1	63	VAL	N	N	119.083	1
709	1	64	LEU	CA	C	56.939	1
710	1	64	LEU	CB	C	38.976	1
711	1	64	LEU	CD1	C	22.162
712	1	64	LEU	CD2	C	22.162
713	1	64	LEU	C	C	177.946	1
714	1	64	LEU	HA	H	4.034	1
715	1	64	LEU	HB2	H	1.606
716	1	64	LEU	HB3	H	1.606
717	1	64	LEU	HD11	H	1.075
718	1	64	LEU	HD12	H	1.075
719	1	64	LEU	HD13	H	1.075
720	1	64	LEU	HD21	H	1.075
721	1	64	LEU	HD22	H	1.075
722	1	64	LEU	HD23	H	1.075
723	1	64	LEU	HG	H	1.622	1
724	1	64	LEU	H	H	6.723	1
725	1	64	LEU	N	N	121.988	1
726	1	65	LYS	CA	C	59.645	1
727	1	65	LYS	CB	C	32.14	1
728	1	65	LYS	CE	C	42.593	1
729	1	65	LYS	CG	C	25.004	1
730	1	65	LYS	HA	H	3.823	1
731	1	65	LYS	HB2	H	1.671
732	1	65	LYS	HB3	H	1.671
733	1	65	LYS	HE2	H	3.076
734	1	65	LYS	HE3	H	3.076
735	1	65	LYS	HG2	H	1.495
736	1	65	LYS	HG3	H	1.495
737	1	65	LYS	H	H	8	1
738	1	65	LYS	N	N	118.648	1
739	1	66	SER	CA	C	61.764	1
740	1	66	SER	CB	C	62.746	1
741	1	66	SER	C	C	177.133	1
742	1	66	SER	HA	H	4.203	1
743	1	66	SER	HB2	H	4.09
744	1	66	SER	HB3	H	4.09
745	1	66	SER	H	H	8.004	1
746	1	66	SER	N	N	113.867	1
747	1	67	LEU	CA	C	58.304	1
748	1	67	LEU	CB	C	41.852	1
749	1	67	LEU	CD1	C	22.748
750	1	67	LEU	CD2	C	26.431
751	1	67	LEU	C	C	179.927	1
752	1	67	LEU	HA	H	4.008	1
753	1	67	LEU	HB3	H	1.529
754	1	67	LEU	HB2	H	1.959
755	1	67	LEU	HD11	H	0.743
756	1	67	LEU	HD12	H	0.743
757	1	67	LEU	HD13	H	0.743
758	1	67	LEU	HD21	H	0.927
759	1	67	LEU	HD22	H	0.927
760	1	67	LEU	HD23	H	0.927
761	1	67	LEU	HG	H	1.893	1
762	1	67	LEU	H	H	7.833	1
763	1	67	LEU	N	N	121.664	1
764	1	68	ILE	CA	C	66.185	1
765	1	68	ILE	CB	C	38.111	1
766	1	68	ILE	CD1	C	13.938	1
767	1	68	ILE	CG1	C	28.871	1
768	1	68	ILE	CG2	C	17.162	1
769	1	68	ILE	HA	H	2.921	1
770	1	68	ILE	HB	H	1.643	1
771	1	68	ILE	HD11	H	0.659	1
772	1	68	ILE	HD12	H	0.659	1
773	1	68	ILE	HD13	H	0.659	1
774	1	68	ILE	HG12	H	-0.568
775	1	68	ILE	HG13	H	-0.568
776	1	68	ILE	HG21	H	0.885	1
777	1	68	ILE	HG22	H	0.885	1
778	1	68	ILE	HG23	H	0.885	1
779	1	68	ILE	H	H	8.606	1
780	1	68	ILE	N	N	121.984	1
781	1	68	ILE	C	C	178.513	1
782	1	69	SER	CA	C	62.702	1
783	1	69	SER	C	C	177.143	1
784	1	69	SER	HA	H	4.119	1
785	1	69	SER	HB2	H	4.107
786	1	69	SER	HB3	H	4.107
787	1	69	SER	H	H	8.486	1
788	1	69	SER	N	N	116.982	1
789	1	70	GLN	CA	C	59.133	1
790	1	70	GLN	CB	C	29.523	1
791	1	70	GLN	CG	C	34.746	1
792	1	70	GLN	C	C	178.486	1
793	1	70	GLN	HA	H	4.117	1
794	1	70	GLN	HB2	H	2.192
795	1	70	GLN	HB3	H	2.192
796	1	70	GLN	HG2	H	2.606
797	1	70	GLN	HG3	H	2.606
798	1	70	GLN	H	H	8.107	1
799	1	70	GLN	N	N	120.46	1
800	1	71	TRP	CA	C	62.45	1
801	1	71	TRP	CB	C	29.562	1
802	1	71	TRP	CD1	C	121.964	1
803	1	71	TRP	CE3	C	115.625	1
804	1	71	TRP	CH2	C	119.519	1
805	1	71	TRP	C	C	179.804	1
806	1	71	TRP	CZ2	C	109.576	1
807	1	71	TRP	CZ3	C	115.55	1
808	1	71	TRP	HA	H	4.224	1
809	1	71	TRP	HB2	H	3.654
810	1	71	TRP	HB3	H	3.654
811	1	71	TRP	HD1	H	7.43	1
812	1	71	TRP	HE1	H	10.282	1
813	1	71	TRP	HH2	H	7.088	1
814	1	71	TRP	H	H	8.321	1
815	1	71	TRP	HZ2	H	7.447	1
816	1	71	TRP	HZ3	H	7.844	1
817	1	71	TRP	N	N	123.502	1
818	1	71	TRP	NE1	N	130.173	1
819	1	72	CYS	CA	C	65.102	1
820	1	72	CYS	CB	C	26.237	1
821	1	72	CYS	C	C	177.164	1
822	1	72	CYS	HA	H	3.843	1
823	1	72	CYS	HB2	H	3.23
824	1	72	CYS	HB3	H	3.23
825	1	72	CYS	H	H	9.224	1
826	1	72	CYS	N	N	119.396	1
827	1	73	GLU	CA	C	59.337	1
828	1	73	GLU	CB	C	29.479	1
829	1	73	GLU	CG	C	36.193	1
830	1	73	GLU	C	C	179.457	1
831	1	73	GLU	HA	H	4.038	1
832	1	73	GLU	HB2	H	2.085
833	1	73	GLU	HB3	H	2.085
834	1	73	GLU	HG2	H	2.336
835	1	73	GLU	HG3	H	2.336
836	1	73	GLU	H	H	8.065	1
837	1	73	GLU	N	N	119.722	1
838	1	74	ALA	CA	C	54.22	1
839	1	74	ALA	CB	C	18.504	1
840	1	74	ALA	C	C	178.573	1
841	1	74	ALA	HA	H	4.091	1
842	1	74	ALA	HB1	H	1.351	1
843	1	74	ALA	HB2	H	1.351	1
844	1	74	ALA	HB3	H	1.351	1
845	1	74	ALA	H	H	7.875	1
846	1	74	ALA	N	N	120.484	1
847	1	75	ASN	CA	C	53.566	1
848	1	75	ASN	CB	C	39.766	1
849	1	75	ASN	C	C	174.15	1
850	1	75	ASN	HA	H	4.521	1
851	1	75	ASN	HB3	H	1.435
852	1	75	ASN	HB2	H	2.311
853	1	75	ASN	HD21	H	6.115
854	1	75	ASN	HD22	H	6.369
855	1	75	ASN	H	H	7.28	1
856	1	75	ASN	N	N	112.903	1
857	1	75	ASN	ND2	N	115.349	1
858	1	76	GLY	CA	C	47.097	1
859	1	76	GLY	C	C	174.111	1
860	1	76	GLY	HA2	H	3.843
861	1	76	GLY	HA3	H	3.843
862	1	76	GLY	H	H	7.505	1
863	1	76	GLY	N	N	108.88	1
864	1	77	ILE	CA	C	59.782	1
865	1	77	ILE	CB	C	39.012	1
866	1	77	ILE	CG1	C	27.394	1
867	1	77	ILE	CG2	C	17.135	1
868	1	77	ILE	C	C	175.19	1
869	1	77	ILE	HA	H	4.096	1
870	1	77	ILE	HB	H	1.624	1
871	1	77	ILE	HD11	H	0.907	1
872	1	77	ILE	HD12	H	0.907	1
873	1	77	ILE	HD13	H	0.907	1
874	1	77	ILE	HG12	H	1.359
875	1	77	ILE	HG13	H	1.359
876	1	77	ILE	HG21	H	0.907	1
877	1	77	ILE	HG22	H	0.907	1
878	1	77	ILE	HG23	H	0.907	1
879	1	77	ILE	H	H	8.044	1
880	1	77	ILE	N	N	120.896	1
881	1	78	GLU	CA	C	55.983	1
882	1	78	GLU	CB	C	30.614	1
883	1	78	GLU	CG	C	36.3	1
884	1	78	GLU	C	C	175.316	1
885	1	78	GLU	HA	H	4.234	1
886	1	78	GLU	HB2	H	1.892
887	1	78	GLU	HB3	H	1.892
888	1	78	GLU	HG2	H	2.164
889	1	78	GLU	HG3	H	2.164
890	1	78	GLU	H	H	8.42	1
891	1	78	GLU	N	N	127.19	1
892	1	79	LEU	CA	C	52.787	1
893	1	79	LEU	CB	C	41.575	1
894	1	79	LEU	CD1	C	23.388
895	1	79	LEU	CD2	C	23.388
896	1	79	LEU	HA	H	4.62	1
897	1	79	LEU	HB2	H	1.649
898	1	79	LEU	HB3	H	1.649
899	1	79	LEU	HD11	H	0.934
900	1	79	LEU	HD12	H	0.934
901	1	79	LEU	HD13	H	0.934
902	1	79	LEU	HD21	H	0.934
903	1	79	LEU	HD22	H	0.934
904	1	79	LEU	HD23	H	0.934
905	1	79	LEU	HG	H	1.628	1
906	1	79	LEU	H	H	8.366	1
907	1	79	LEU	N	N	126.614	1
908	1	80	PRO	CD	C	50.304	1
909	1	80	PRO	CG	C	27.525	1
910	1	80	PRO	HA	H	4.317	1
911	1	80	PRO	HB2	H	1.619
912	1	80	PRO	HB3	H	1.619
913	1	80	PRO	HD3	H	3.621
914	1	80	PRO	HD2	H	3.795
915	1	80	PRO	HG2	H	1.961
916	1	80	PRO	HG3	H	1.961

Release date

2023-08-01

Citation

Release of a Ubiquitin Brake Activates Pattern Recognition Receptor-mediated Immunity in Rice
Zhang, Y., Yu, C.Z., Lan, W.X.

Related entities 1. U-box domain-containing protein 12 (E.C.2.3.2.27), : 1 : 1 : 3 : 225 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Agilent DD2 - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.5 mM [U-100% 13C; U-100% 15N] WT-Ubox, 10 mM TRIS, 100 mM sodium chloride, 1 mM PMSF, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

3 2D 1H-13C HSQC aromatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

4 3D 1H-13C NOESY aliphatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

5 3D 1H-13C NOESY aromatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

6 3D 1H-15N NOESY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

7 3D 1H-15N TOCSY

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

8 3D HCCH-TOCSY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

9 3D HNCO

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

10 3D HNCA

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

11 3D HNCACB

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

12 3D CBCA(CO)NH

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36528_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36528_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	starch_output	Warning	916		97.5 (chain: 1, length: 80)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: starch_output
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 80, Sequence coverage: 97.5%
- Total number of assignments
  - ¹³C chemical shifts: 305
  - ¹H chemical shifts: 539
  - ¹⁵N chemical shifts: 72
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
30	TYR	HH	9.478

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	365	305	83.6
¹H chemical shifts	477	438	91.8
¹⁵N chemical shifts	83	72	86.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	160	138	86.3
¹H chemical shifts	157	144	91.7
¹⁵N chemical shifts	73	67	91.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	205	167	81.5
¹H chemical shifts	320	294	91.9
¹⁵N chemical shifts	10	5	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	45	40	88.9
¹H chemical shifts	45	43	95.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	28	24	85.7
¹H chemical shifts	30	27	90.0
¹⁵N chemical shifts	2	2	100.0

Keywords PLANT PROTEIN, Ubox, ligase, ubiquitination

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Found 1 Document (0.552 seconds)

BMRB: 36528

Related entities 1. U-box domain-containing protein 12 (E.C.2.3.2.27), : 1 : 1 : 3 : 225 entities Detail

Experiments performed 12 experiments Detail

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Chemical shift validation 4 contents Detail