Main-Chain NMR Assignments for AsiA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 48.7 % (538 of 1104) | 33.8 % (195 of 577) | 60.6 % (258 of 426) | 84.2 % (85 of 101) |
Backbone | 93.7 % (506 of 540) | 92.4 % (170 of 184) | 94.4 % (251 of 266) | 94.4 % (85 of 90) |
Sidechain | 17.2 % (112 of 650) | 6.6 % (26 of 393) | 35.0 % (86 of 246) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 88) | 0.0 % (0 of 44) | 0.0 % (0 of 44) | |
Methyl | 10.4 % (11 of 106) | 9.4 % (5 of 53) | 11.3 % (6 of 53) |
1. Anti-sigma-A
MNKNIDTVRE IITVASILIK FSREDIVENR ANFIAFLNEI GVTHEGRKLN QNSFRKIVSE LTQEDKKTLI DEFNEGFEGV YRYLEMYTNKTemperature 298 (±0.1) K, pH 6.0 (±0.02), Details 600 uL NMR sample, 13C, 15N uniform labeling, 1.5 mM AsiA, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Anti-sigma-A | [U-99% 13C; U-99% 15N] | 1.5 mM | |
2 | potassium phosphate | 50 mM | ||
3 | sodium azide | 0.02 % | ||
4 | D2O | 10.0 % | ||
5 | H2O | 90.0 % |
Temperature 298 (±0.1) K, pH 6.0 (±0.02), Details 600 uL NMR sample, 15N uniform labeling, 2.0 mM AsiA, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Anti-sigma-A | [U-99% 15N] | 2.0 mM | |
7 | potassium phosphate | 50 mM | ||
8 | sodium azide | 0.02 % | ||
9 | D2O | 10.0 % | ||
10 | H2O | 90.0 % |