BMRBj - BMREntryMaster

Found 1 Document (1.075 seconds)

BMRB: 4072

2IJK

Sequence Specific 1HA, 15N, 13CA, and 13CB Assignments for RNA-1 Modulator Protein ROM

Authors

Pelton, J.G., Chang, K., Comolli, L.R.

Assembly

RNA-1 Modulator Protein

Entity

1. RNA-1 Modulator Protein (polymer), 63 monomers, 7153.820 Da Detail

GTKQEKTALN MARFIRSQTL TLLEKLNELD ADEQADICES LHDHADELYR SCLARFGDDG ENL

Formula weight

7153.82 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 42.7 %, Completeness (bb): 75.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.7 % (304 of 712)	34.9 % (129 of 370)	41.8 % (114 of 273)	88.4 % (61 of 69)
Backbone	75.1 % (284 of 378)	89.1 % (115 of 129)	60.2 % (112 of 186)	90.5 % (57 of 63)
Sidechain	18.5 % (73 of 394)	5.8 % (14 of 241)	37.4 % (55 of 147)	66.7 % (4 of 6)
Aromatic	22.2 % (8 of 36)	33.3 % (6 of 18)	11.1 % (2 of 18)
Methyl	8.8 % (6 of 68)	0.0 % (0 of 34)	17.6 % (6 of 34)

1. RNA-1 Modulator Protein

GTKQEKTALN MARFIRSQTL TLLEKLNELD ADEQADICES LHDHADELYR SCLARFGDDG ENL

Show sample condition

Sample

Pressure 1.0 (±0.1) atm, Temperature 303 (±1.0) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	RNA-1 Modulator Protein	[U-15N; U-13C]	2 mM
2	phosphate_buffer		10 mM
3	dithiothreitol		1 mM
4	EDTA		1 mM

Release date

1998-02-24

Citation 1

Sequence specific 1HN, 15N, 1H alpha, 13C alpha, and 13C beta assignments for RNA-1 modulator protein ROM
Pelton, J.G., Chang, K., Comolli, L.R.
J. Biomol. NMR (1998), 11, 463-464, PubMed 9691290 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

Hide non-primary 1 citaion

Related entities 1. RNA-1 Modulator Protein, : 1 : 2 : 10 : 8 entities Detail

Interaction partners 1. RNA-1 Modulator Protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4072_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4072_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_ROM_1	Warning	303		90.5 (chain: 1, length: 63)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_ROM_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 63, Sequence coverage: 90.5%
- Total number of assignments
  - ¹H chemical shifts: 129
  - ¹⁵N chemical shifts: 61
  - ¹³C chemical shifts: 113
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	370	129	34.9
¹⁵N chemical shifts	73	61	83.6
¹³C chemical shifts	273	113	41.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	129	115	89.1
¹⁵N chemical shifts	63	57	90.5
¹³C chemical shifts	126	57	45.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	241	14	5.8
¹⁵N chemical shifts	10	4	40.0
¹³C chemical shifts	147	56	38.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	35	0	0.0
¹³C chemical shifts	35	6	17.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	6	33.3
¹³C chemical shifts	18	2	11.1

Keywords RNA-Binding Protein, ROP

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Found 1 Document (1.075 seconds)

BMRB: 4072

Sample

Related entities 1. RNA-1 Modulator Protein, : 1 : 2 : 10 : 8 entities Detail

Interaction partners 1. RNA-1 Modulator Protein, : 1 interactors Detail

Chemical shift validation 3 contents Detail