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Found 1 Document (1.383 seconds)

BMRB: 4075

1B79

Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli

Authors

Weigelt, J., Miles, C.S., Dixon, N.E., Otting, G.

Assembly

DnaB helicase

Entity

1. DnaB helicase (polymer), 142 monomers, 15700.35 Da Detail

AGNKPFNKQQ AEPRERDPQV AGLKVPPHSI EAEQSVLGGL MLDNERWDDV AERVVADDFY TRPHRHIFTE MARLQESGSP IDLITLAESL ERQGQLDSVG GFAYLAELSK NTPSAANISA YADIVRERAV VREMISVANE IA

Formula weight

15700.35 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.7 %, Completeness: 45.4 %, Completeness (bb): 79.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.4 % (729 of 1604)	37.1 % (310 of 835)	47.3 % (294 of 621)	84.5 % (125 of 148)
Backbone	79.4 % (664 of 836)	91.9 % (261 of 284)	66.7 % (279 of 418)	92.5 % (124 of 134)
Sidechain	9.4 % (85 of 902)	8.9 % (49 of 551)	10.7 % (36 of 337)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 88)	0.0 % (0 of 44)	0.0 % (0 of 43)	0.0 % (0 of 1)
Methyl	13.0 % (21 of 162)	12.3 % (10 of 81)	13.6 % (11 of 81)

1. DnaB helicase

AGNKPFNKQQ AEPRERDPQV AGLKVPPHSI EAEQSVLGGL MLDNERWDDV AERVVADDFY TRPHRHIFTE MARLQESGSP IDLITLAESL ERQGQLDSVG GFAYLAELSK NTPSAANISA YADIVRERAV VREMISVANE IA

Show sample condition

Sample

Temperature 305 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	DnaB helicase	[U-13C;U-15N]	1.8 mM
2	sodium phosphate		20 mM
3	pefabloc		1 mM
4	EDTA		1 mM
5	NaN3		0.02 % w/v

Release date

1998-10-11

Citation 1

Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli
Weigelt, J., Miles, C.S., Dixon, N.E., Otting, G.
J. Biomol. NMR (1998), 11, 233-234

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. DnaB helicase, : 1 : 2 : 1 : 36 entities Detail

Interaction partners 1. DnaB helicase, : 20 interactors Detail

More details for 20 interactors identified by 8 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4075_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4075_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment	OK	636		93.0 (chain: 1, length: 142)

Assigned chemical shifts

Saveframe: chemical_shift_assignment
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 142, Sequence coverage: 93.0%
- Total number of assignments
  - ¹H chemical shifts: 259
  - ¹³C chemical shifts: 253
  - ¹⁵N chemical shifts: 124
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	835	259	31.0
¹³C chemical shifts	621	253	40.7
¹⁵N chemical shifts	159	124	78.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	284	259	91.2
¹³C chemical shifts	284	253	89.1
¹⁵N chemical shifts	134	124	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	551	0	0.0
¹³C chemical shifts	337	0	0.0
¹⁵N chemical shifts	25	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	84	0	0.0
¹³C chemical shifts	84	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	0	0.0
¹³C chemical shifts	43	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords DnaB helicase

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Found 1 Document (1.383 seconds)

BMRB: 4075

Sample

Related entities 1. DnaB helicase, : 1 : 2 : 1 : 36 entities Detail

Interaction partners 1. DnaB helicase, : 20 interactors Detail

Chemical shift validation 3 contents Detail