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Found 1 Document (1.573 seconds)

BMRB: 4127

1TBD

Sequence-specific 1H, 13C, and 15N Resonance Assignment of the Origin DNA-binding Domain of SV40 T Antigen

Authors

Luo, X., Sanford, D.G., Bullock, P.A., Bachovchin, W.W.

Assembly

Origin DNA-binding domain of SV40 T-antigen

Entity

1. Origin DNA-binding domain of SV40 T-antigen (polymer), 134 monomers, 15392.44 Da Detail

GSKVEDPKDF PSELLSFLSH AVFSNRTLAC FAIYTTKEKA ALLYKKIMEK YSVTFISRHN SYNHNILFFL TPHRHRVSAI NNYAQKLCTF SFLICKGVNK EYLMYSALTR DPFSVIEESL PGGLKEHDFN PESS

Formula weight

15392.44 Da

Entity Connection

peptide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	peptide	sing	1:ASP111:C	1:PRO112:N

Source organism

Macaca mulatta polyomavirus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.7 %, Completeness (bb): 92.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (1374 of 1622)	88.7 % (746 of 841)	77.0 % (496 of 644)	96.4 % (132 of 137)
Backbone	92.8 % (735 of 792)	97.0 % (258 of 266)	88.7 % (353 of 398)	96.9 % (124 of 128)
Sidechain	76.7 % (736 of 960)	84.9 % (488 of 575)	63.8 % (240 of 376)	88.9 % (8 of 9)
Aromatic	49.5 % (94 of 190)	60.0 % (57 of 95)	38.9 % (37 of 95)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. Origin DNA-binding domain of SV40 T-antigen

GSKVEDPKDF PSELLSFLSH AVFSNRTLAC FAIYTTKEKA ALLYKKIMEK YSVTFISRHN SYNHNILFFL TPHRHRVSAI NNYAQKLCTF SFLICKGVNK EYLMYSALTR DPFSVIEESL PGGLKEHDFN PESS

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Origin DNA-binding domain of SV40 T-antigen	[U-15N;U-13C]		3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbon	0.0 ppm	null	null
¹H	H2O	protons	4.72 ppm	null	null
¹⁵N	liquid NH3	nitrogen	0.0 ppm	null	null

Referencing offset: 2.8 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbon	0.0 ppm	null	null
¹H	H2O	protons	4.72 ppm	null	null
¹⁵N	liquid NH3	nitrogen	0.0 ppm	null	null

Referencing offset: 2.8 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbon	0.0 ppm	null	null
¹H	H2O	protons	4.72 ppm	null	null
¹⁵N	liquid NH3	nitrogen	0.0 ppm	null	null

Referencing offset: 0.03 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl carbon	0.0 ppm	null	null
¹H	H2O	protons	4.72 ppm	null	null
¹⁵N	liquid NH3	nitrogen	0.0 ppm	null	null

Referencing offset: 3.23 ppm, Outliers: 1 Detail

Release date

1999-03-03

Citation

Solution structure of the origin DNA-binding domain of SV40 T-antigen
Luo, X., Sanford, D.G., Bullock, P.A., Bachovchin, W.W.
Nat. Struct. Biol. (1996), 3, 1034-1039, PubMed 8946857 , DOI 10.1038/nsb1296-1034 , Abstract

Related entities 1. Origin DNA-binding domain of SV40 T-antigen, : 1 : 3 : 10 : 23 entities Detail

Interaction partners 1. Origin DNA-binding domain of SV40 T-antigen, : 65 interactors Detail

More details for 65 interactors identified by 24 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4127_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:111:ASP:C	1:112:PRO:N	unknown	unknown	n/a

Non-standard residues

None

Content subtype: bmr4127_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	Warning	1490		98.5 (chain: 1, length: 134)
1	Chemical shift references	chemical_shift_reference_one	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 134, Sequence coverage: 98.5%
- Total number of assignments
  - ¹H chemical shifts: 885
  - ¹³C chemical shifts: 475
  - ¹⁵N chemical shifts: 130
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
44	TYR	HH	5.96

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	841	742	88.2
¹³C chemical shifts	644	475	73.8
¹⁵N chemical shifts	142	130	91.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	266	257	96.6
¹³C chemical shifts	268	251	93.7
¹⁵N chemical shifts	128	122	95.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	575	485	84.3
¹³C chemical shifts	376	224	59.6
¹⁵N chemical shifts	14	8	57.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	71	100.0
¹³C chemical shifts	71	71	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	95	56	58.9
¹³C chemical shifts	95	36	37.9

Keywords NMR structure, resonance assignment, SV40 T antigen DNA-binding domain

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Found 1 Document (1.573 seconds)

BMRB: 4127

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Origin DNA-binding domain of SV40 T-antigen, : 1 : 3 : 10 : 23 entities Detail

Interaction partners 1. Origin DNA-binding domain of SV40 T-antigen, : 65 interactors Detail

Chemical shift validation 3 contents Detail