1H, 15N, 13C chemical shifts of recombinant rat ferrocytochrome b5, B conformation
DKDVKYYTLE EIQKHKDSKS TWVILHHKVY DLTKFLEEHP GGEEVLREQA GGDATENFED VGHSTDAREL SKTYIIGELH PDDRSKIAKP SETL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.5 % (829 of 1098) | 76.4 % (437 of 572) | 70.1 % (302 of 431) | 94.7 % (90 of 95) |
Backbone | 94.4 % (527 of 558) | 97.4 % (186 of 191) | 90.9 % (251 of 276) | 98.9 % (90 of 91) |
Sidechain | 59.7 % (375 of 628) | 65.9 % (251 of 381) | 51.0 % (124 of 243) | 0.0 % (0 of 4) |
Aromatic | 14.8 % (13 of 88) | 29.5 % (13 of 44) | 0.0 % (0 of 43) | 0.0 % (0 of 1) |
Methyl | 77.7 % (73 of 94) | 87.2 % (41 of 47) | 68.1 % (32 of 47) |
1. cytochrome b5
DKDVKYYTLE EIQKHKDSKS TWVILHHKVY DLTKFLEEHP GGEEVLREQA GGDATENFED VGHSTDAREL SKTYIIGELH PDDRSKIAKP SETLTemperature 313 (±1) K, pH 7.0 (±0.2), Details 4mM 15N, 13C labeled in 100 mM phosphate pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | rat ferrocytochrome b5 B form | [U-15N,; U-13C] | 4 mM | |
2 | phosphate | 100 mM |