BMRB: 4147

Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein

Authors

Kloks, C.P.A.M., Hoffmann, A., Omichinski, J.G., Vuister, G.W., Hilbers, C.W., Grzesiek, S.

Assembly

Cold Shock domain

Entity

1. Cold Shock domain (polymer), 79 monomers, 8740.782 Da Detail

MKKVIATKVL GTVKWFNVRN GYGFINRNDT KEDVFVHQTA IKKNNPRKYL RSVGDGETVE FDVVEGEKGA EAANVTGPG

Formula weight

8740.782 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS FOLLOWED BY RESTRAINED MD

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 79.2 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.2 % (726 of 917)	76.9 % (367 of 477)	81.1 % (287 of 354)	83.7 % (72 of 86)
Backbone	91.7 % (431 of 470)	92.7 % (153 of 165)	91.2 % (208 of 228)	90.9 % (70 of 77)
Sidechain	69.6 % (360 of 517)	68.6 % (214 of 312)	73.5 % (144 of 196)	22.2 % (2 of 9)
Aromatic	4.2 % (3 of 72)	5.6 % (2 of 36)	0.0 % (0 of 35)	100.0 % (1 of 1)
Methyl	93.0 % (80 of 86)	93.0 % (40 of 43)	93.0 % (40 of 43)

1. Cold Shock domain

MKKVIATKVL GTVKWFNVRN GYGFINRNDT KEDVFVHQTA IKKNNPRKYL RSVGDGETVE FDVVEGEKGA EAANVTGPG

Show sample condition

Sample #1

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	Cold Shock domain	[U-15N]		1.5 mM
2	H2O			90 %
3	D2O			10 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
4	Cold Shock domain	[U-15N;U-13C]		1.5 mM
5	H2O			90 %
6	D2O			10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
7	Cold Shock domain	[U-15N;U-13C]		1.5 mM
8	D2O			100 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	null	direct	0.0
15N	liquid NH3	na	0.0 ppm	null	indirect	0.0

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	null	direct	0.0
15N	liquid NH3	na	0.0 ppm	null	indirect	0.0

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	null	direct	0.0
15N	liquid NH3	na	0.0 ppm	null	indirect	0.0

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	null	direct	0.0
15N	liquid NH3	na	0.0 ppm	null	indirect	0.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 1H95, Strand ID: A Detail

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Coupling constant

70 J values in 1 lists
Field strength (¹H) 600 MHz, Temperature 303 K, pH 6.7 Detail

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Temperature 303 K, pH 6.7

List #1 coupling_constants_one, Field strength (¹H) 600 MHz, Details Correction factor: 5% 3Jhnha

Release date

1999-02-06

Citation

Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein
Kloks, C.P.A.M., Hoffmann, A., Omichinski, J.G., Vuister, G.W., Hilbers, C.W., Grzesiek, S.
J. Biomol. NMR (1998), 12, 463-464, PubMed , DOI:

Related entities 1. Cold Shock domain, : 1 : 21 : 198 entities Detail

Interaction partners 1. Cold Shock domain, : 7 interactors Detail

Experiments performed 1 experiments Detail