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BMRB: 4155

1EOT

Solution Structure of Eotaxin: A Chemokine that Selectively Recruits Eosinophils in Allergic Inflammation

Authors

Crump, M.P., Rajarathnam, K., Kim, K., Sykes, B.D.

Assembly

Eotaxin

Entity

1. Eotaxin (polymer), 74 monomers, 8418.852 Da Detail

XPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

Formula weight

8418.852 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS9:SG	1:CYS34:SG
2	disulfide	sing	0:CYS10:SG	1:CYS50:SG

Source organism

Exptl. method

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 85.5 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	85.5 % (473 of 553)	85.0 % (409 of 481)	88.9 % (64 of 72)
Backbone	98.0 % (200 of 204)	97.8 % (136 of 139)	98.5 % (64 of 65)
Sidechain	78.2 % (273 of 349)	79.8 % (273 of 342)	0.0 % (0 of 7)
Aromatic	80.0 % (20 of 25)	83.3 % (20 of 24)	0.0 % (0 of 1)
Methyl	100.0 % (34 of 34)	100.0 % (34 of 34)

1. Eotaxin

XPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 1EOT, Strand ID: A Detail

Coupling constant

56 J values in 1 lists
Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1) Detail

Release date

1999-02-28

Citation 1

Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation
Crump, M.P., Rajarathnam, K., Kim, K., Sykes, B.D.
J. Biol. Chem. (1998), 273, 22471-22479, PubMed 9712872 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Eotaxin, : 1 : 5 : 247 entities Detail

Interaction partners 1. Eotaxin, : 27 interactors Detail

More details for 27 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 DQF-COSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

2 TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

3 NOESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

4 ROESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

5 13C-HSQC (natural abundance)

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

6 1H-15N HSQC'

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

7 15N edited TOCSY HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

8 15N edited NOESY HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

9 HNHA

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

10 The 13C HSQC was collected to check methyl proton

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

11 13C/1H shifts. The 13C eotaxin shifts are not reported

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

12 in this deposition.

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 303 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eotaxin	[U-15N]		1.0 mM
2	sodium azide			1.0 mM
3	sodium acetate			20.0 mM
4	DSS			1.0 mM

nullKeywords ccr3, chemokine, cytokine, eosinophil, eotaxin, protein synthesis, solution structure