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BMRB: 4161

2IRF

1H, 13C and 15N Assignment of the DNA Binding Domain of Interferon Regulatory Factor-2

Authors

Furui, J., Uegaki, K., Yamazaki, T., Shirakawa, M., Harada, H., Taniguchi, T., Kyogoku, Y.

Assembly

IRF-2 DNA binding domain

Entity

1. IRF-2 DNA binding domain (polymer, Thiol state: not reported), 113 monomers, 13400.39 Da Detail

PVERMRMRPW LEEQINSNTI PGLKWLNKEK KIFQIPWMHA ARHGWDVEKD APLFRNWAIH TGKHQPGIDK PDPKTWKANF RCAMNSLPDI EEVKDRSIKK GNNAFRVYRM LPA

Formula weight

13400.39 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 84.1 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.1 % (1194 of 1420)	84.4 % (639 of 757)	80.3 % (436 of 543)	99.2 % (119 of 120)
Backbone	97.6 % (642 of 658)	99.1 % (219 of 221)	96.1 % (321 of 334)	99.0 % (102 of 103)
Sidechain	75.5 % (657 of 870)	78.4 % (420 of 536)	69.4 % (220 of 317)	100.0 % (17 of 17)
Aromatic	52.2 % (71 of 136)	85.3 % (58 of 68)	11.3 % (7 of 62)	100.0 % (6 of 6)
Methyl	93.6 % (88 of 94)	93.6 % (44 of 47)	93.6 % (44 of 47)

1. IRF-2 DNA binding domain

PVERMRMRPW LEEQINSNTI PGLKWLNKEK KIFQIPWMHA ARHGWDVEKD APLFRNWAIH TGKHQPGIDK PDPKTWKANF RCAMNSLPDI EEVKDRSIKK GNNAFRVYRM LPA

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	IRF-2 DNA binding domain	[U-13C; U-15N]		0.5 ~ 1.5 mM

Sample #2

Temperature 298 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	IRF-2 DNA binding domain	[U-15N]		0.5 ~ 1.0 mM

Sample #3

Temperature 298 (±1) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	IRF-2 DNA binding domain	[2H; HD1, HE3, HZ2, HZ3, HH2]-Trp		1.0 mM
4	IRF-2 DNA binding domain	[2H; HD1, HD2, HE1, HE2, HZ]-Phe		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.52 ppm, Outliers: 2 Detail

Release date

2000-03-20

Citation 1

Solution structure of the IRF-2 DNA-binding domain: a novel subgroup of the winged helix-turn-helix family
Furui, J., Uegaki, K., Yamazaki, T., Shirakawa, M., Harada, H., Taniguchi, T., Kyogoku, Y.
Structure (1998), 6, 491-500, PubMed 9562558 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. IRF-2 DNA binding domain, : 1 : 5 : 79 entities Detail

Interaction partners 1. IRF-2 DNA binding domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 HNCA