BMRBj - BMREntryMaster

Found 1 Document (0.777 seconds)

BMRB: 4166

5W5C

Backbone H, CA, N Chemical Shift Assignments for C2A/Ca2+ Binary Complex

Authors

Chae, Y.K., Markley, J.L.

Assembly

C2A domain of synaptotagmin I

Entity

1. C2A domain of synaptotagmin I (polymer, Thiol state: not present), 128 monomers, 14777.73 Da Detail

EKLGKLQYSL DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPEK KKKFETKVHR KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT VDFGHVTEEW RDLQSAEK

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

14817.809 Da

Max. entity weight

14777.73 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 24.3 %, Completeness (bb): 49.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	24.3 % (383 of 1576)	15.9 % (131 of 823)	22.0 % (136 of 619)	86.6 % (116 of 134)
Backbone	49.1 % (371 of 756)	46.9 % (121 of 258)	35.6 % (134 of 376)	95.1 % (116 of 122)
Sidechain	1.9 % (18 of 940)	1.8 % (10 of 565)	2.2 % (8 of 363)	0.0 % (0 of 12)
Aromatic	1.2 % (2 of 164)	2.4 % (2 of 82)	0.0 % (0 of 81)	0.0 % (0 of 1)
Methyl	2.9 % (4 of 136)	2.9 % (2 of 68)	2.9 % (2 of 68)

1. C2A domain of synaptotagmin I

EKLGKLQYSL DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPEK KKKFETKVHR KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT VDFGHVTEEW RDLQSAEK

Show sample condition

Sample

Temperature 303 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	C2A domain of synaptotagmin I	[U-99% 13C; U-99% 15N]		1.0 mM

Release date

2000-03-26

Citation 1

Lipid-induced Local Conformational Changes in the C2A-domain of Synaptotagmin I as Revealed by NMR Spectroscopy
Chae, Y.K., Markley, J.L.
J. Biol. Chem. (1998), 273, 25659-25663

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. C2A domain of synaptotagmin I, : 2 : 14 : 416 entities Detail

Interaction partners 1. C2A domain of synaptotagmin I, : 11 interactors Detail

More details for 11 interactors identified by 10 citations

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4166_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4166_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	Warning	355		97.7 (chain: 1, length: 128)
1	Chemical shift references	chemical_shift_reference_one	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 128, Sequence coverage: 97.7%
- Total number of assignments
  - ¹³C chemical shifts: 125
  - ¹H chemical shifts: 115
  - ¹⁵N chemical shifts: 115
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	619	125	20.2
¹H chemical shifts	823	115	14.0
¹⁵N chemical shifts	137	115	83.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	256	125	48.8
¹H chemical shifts	258	115	44.6
¹⁵N chemical shifts	122	115	94.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	363	0	0.0
¹H chemical shifts	565	0	0.0
¹⁵N chemical shifts	15	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	71	0	0.0
¹H chemical shifts	71	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	81	0	0.0
¹H chemical shifts	82	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords C2A domain, conformational change, lipid binding

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Found 1 Document (0.777 seconds)

BMRB: 4166

Sample

Related entities 1. C2A domain of synaptotagmin I, : 2 : 14 : 416 entities Detail

Interaction partners 1. C2A domain of synaptotagmin I, : 11 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail