BMRB: 4167

Backbone H, CA, N chemical shift assignments for C2A/Ca2+/6PS ternary complex

Authors

Chae, Y.K., Markley, J.L.

Assembly

C2A domain of synaptotagmin I

Entity

1. C2A domain of synaptotagmin I (polymer, Thiol state: not present), 128 monomers, 14777.73 Da Detail

EKLGKLQYSL DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPEK KKKFETKVHR KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT VDFGHVTEEW RDLQSAEK

2. CALCIUM ION (non-polymer), 40.078 Da
3. na (non-polymer), 1 monomers, 283.391 Da

Total weight

15101.199 Da

Max. entity weight

14777.73 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 28.4 %, Completeness (bb): 53.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	28.4 % (447 of 1576)	20.2 % (166 of 823)	25.8 % (160 of 619)	90.3 % (121 of 134)
Backbone	53.7 % (406 of 756)	52.3 % (135 of 258)	39.9 % (150 of 376)	99.2 % (121 of 122)
Sidechain	5.4 % (51 of 940)	5.5 % (31 of 565)	5.5 % (20 of 363)	0.0 % (0 of 12)
Aromatic	2.4 % (4 of 164)	4.9 % (4 of 82)	0.0 % (0 of 81)	0.0 % (0 of 1)
Methyl	10.3 % (14 of 136)	11.8 % (8 of 68)	8.8 % (6 of 68)

1. C2A domain of synaptotagmin I

EKLGKLQYSL DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPEK KKKFETKVHR KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT VDFGHVTEEW RDLQSAEK

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Sample

Temperature 303 (±1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	C2A domain of synaptotagmin I	[U-99% 13C; U-99% 15N]		1.0 mM
2	Calcium			5 mM
3	na			5 mM
4	Tris-HCl			50 mM
5	NaCl			200 mM
6	DSS			2 mM

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Release date

2000-03-26

Citation 1

Lipid-induced Local Conformational Changes in the C2A-domain of Synaptotagmin I as Revealed by NMR Spectroscopy
Chae, Y.K., Markley, J.L.
J. Biol. Chem. (1998), 273, 25659-25663

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

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Related entities 1. C2A domain of synaptotagmin I, : 2 : 14 : 416 entities Detail

Interaction partners 1. C2A domain of synaptotagmin I, : 11 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 5 contents Detail