BMRB: 4174

Chemical Shift Assignments of the Mg2+-Loaded State of the N-Terminal Domain of Calmodulin

Authors

Malmendal, A., Evenas, J., Thulin, E., Gippert, G.P., Drakenberg, T., Forsen, S.

Assembly

N-terminal domain of calmodulin

Entity

1. N-terminal domain of calmodulin (polymer, Thiol state: not present), 81 monomers, 9038.946 Da Detail

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT D

2. MG (non-polymer), 24.305 Da

Total weight

9063.251 Da

Max. entity weight

9038.946 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 96.1 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	96.1 % (537 of 559)	95.6 % (452 of 473)	98.8 % (85 of 86)
Backbone	98.0 % (240 of 245)	97.6 % (162 of 166)	98.7 % (78 of 79)
Sidechain	94.6 % (297 of 314)	94.5 % (290 of 307)	100.0 % (7 of 7)
Aromatic	100.0 % (25 of 25)	100.0 % (25 of 25)
Methyl	97.4 % (38 of 39)	97.4 % (38 of 39)

1. N-terminal domain of calmodulin

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT D

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.2 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of calmodulin			4.0 mM
2	D2O			10 %
3	H2O			90 %
4	KCL			10 mM
5	Azide			100 uM

Sample #2

Temperature 298 (±1) K, pH 7.2 (±0.05)

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of calmodulin	[U-15N]		4.0 mM
7	D2O			10 %
8	H2O			90 %
9	KCL			10 mM
10	Azide			100 uM

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Release date

2000-04-02

Citation 1

When Size is Important: Accommodation of Magnesium in a Calcium Binding Regulatory Domain
Malmendal, A., Evenas, J., Thulin, E., Gippert, G.P., Drakenberg, T., Forsen, S.
J. Biol. Chem. (1998), 273, 28994-29001

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. N-terminal domain of calmodulin, : 2 : 124 : 12 : 6 : 416 entities Detail

Interaction partners 1. N-terminal domain of calmodulin, : 1 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail