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Found 1 Document (4.98 seconds)

BMRB: 4184

1EH2

Structure and Asn-Pro-Phe Binding Pocket of the Eps15 Homology Domain

Authors

De Beer, T., Carter, R.E., Lobel-Rice, K.E., Sorkin, A., Overduin, M.

Assembly

eps15 homology domain

Entity

1. eps15 homology domain (polymer), 95 monomers, 10550.16 Da Detail

PWAVKPEDKA KYDAIFDSLS PVNGFLSGDK VKPVLLNSKL PVDILGRVWE LSDIDHDGML DRDEFAVAMF LVYCALEKEP VPMSLPPALV PPSKR

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

10590.238 Da

Max. entity weight

10550.16 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.3 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (1080 of 1122)	95.7 % (560 of 585)	96.2 % (432 of 449)	100.0 % (88 of 88)
Backbone	97.1 % (532 of 548)	96.2 % (176 of 183)	96.8 % (272 of 281)	100.0 % (84 of 84)
Sidechain	96.2 % (640 of 665)	95.5 % (384 of 402)	97.3 % (252 of 259)	100.0 % (4 of 4)
Aromatic	92.9 % (78 of 84)	97.6 % (41 of 42)	87.5 % (35 of 40)	100.0 % (2 of 2)
Methyl	99.1 % (113 of 114)	98.2 % (56 of 57)	100.0 % (57 of 57)

1. eps15 homology domain

PWAVKPEDKA KYDAIFDSLS PVNGFLSGDK VKPVLLNSKL PVDILGRVWE LSDIDHDGML DRDEFAVAMF LVYCALEKEP VPMSLPPALV PPSKR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±2) K, pH 7.0 (±0.1), Details homonuclear EH2 and U-98% 15N-labelled EH2 samples were measured under similar conditions. DTT is essential for prevention of Cys oxidation

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

Sample #2

#	Name	Isotope labeling	Concentration
9	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
10	Tris		20 mM
11	KCl		100 mM
12	CaCl2		2 mM
13	DTT		2 mM
14	NaN3		10 uM
15	APMSF		0.0 ~ 0.0 na
16	D2O		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0
¹H	DSS	methyl protons	0.0 ppm	null	direct	1.0
¹⁵N	DSS	methyl protons	4.75 ppm	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0
¹H	DSS	methyl protons	0.0 ppm	null	direct	1.0
¹⁵N	DSS	methyl protons	4.75 ppm	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0
¹H	DSS	methyl protons	0.0 ppm	null	direct	1.0
¹⁵N	DSS	methyl protons	4.75 ppm	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0
¹H	DSS	methyl protons	0.0 ppm	null	direct	1.0
¹⁵N	DSS	methyl protons	4.75 ppm	null	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1EH2, Strand ID: A Detail

Release date

2000-01-10

Citation 1

Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain
De Beer, T., Carter, R.E., Lobel-Rice, K.E., Sorkin, A., Overduin, M.
Science (1998), 281, 1357-1360, PubMed 9721102 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. eps15 homology domain, : 1 : 5 : 289 entities Detail

Interaction partners 1. eps15 homology domain, : 72 interactors Detail

More details for 72 interactors identified by 27 citations

Experiments performed 12 experiments Detail

1 15N-filtered TOCSY and NOESY

Instrument

Varian Inova - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±2) K, pH 7.0 (±0.1), Details homonuclear EH2 and U-98% 15N-labelled EH2 samples were measured under similar conditions. DTT is essential for prevention of Cys oxidation

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

2 15N-1H HSQC-NOESY-HSQC

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

3 1H-15N HMQC-J

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

4 3D and 4D 13C/15N-filtered NOESY's

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

5 HNCO

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

6 CCC-TOCSY-NNH

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

7 HCC-TOCSY-NNH

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

8 HBCBCACONNH

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

9 HNCACB

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

10 HCCH-TOCSY

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

11 HBCBCGCDHD

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

12 HBCBCGCDCEHE

Instrument

Varian Inova - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eps15 homology domain	[U-13C; U-98% 15N]	1.0 ~ 4.0 mM
2	Tris		20 mM
3	KCl		100 mM
4	CaCl2		2 mM
5	DTT		2 mM
6	NaN3		10 uM
7	APMSF		0.0 ~ 0.0 na
8	H2O		1 mM

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Found 1 Document (4.98 seconds)

BMRB: 4184

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. eps15 homology domain, : 1 : 5 : 289 entities Detail

Interaction partners 1. eps15 homology domain, : 72 interactors Detail

Experiments performed 12 experiments Detail

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