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BMRB: 4205

2JV3

Structure of the Ets-1 Pointed Domain and Mitogen-activiated Protein Kinase Phosphorylation Site.

Authors

Slupsky, C.M., Gentile, L.N., Donaldson, L.W., Mackereth, C.D., Seidel, J.J., Graves, B.J., Mcintosh, L.P.

Assembly

Ets-1 Pointed

Entity

1. Ets-1 Pointed (polymer), 110 monomers, 12553.40 Da Detail

MECADVPLLT PSSKEMMSQA LKATFSGFTK EQQRLGIPKD PRQWTETHVR DWVMWAVNEF SLKGVDFQKF CMSGAALCAL GKECFLELAP DFVGDILWEH LEILQKEDVK

Formula weight

12553.4 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 93.4 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.4 % (1231 of 1318)	93.6 % (642 of 686)	92.4 % (477 of 516)	96.6 % (112 of 116)
Backbone	95.4 % (620 of 650)	95.9 % (212 of 221)	94.8 % (307 of 324)	96.2 % (101 of 105)
Sidechain	92.4 % (713 of 772)	92.5 % (430 of 465)	91.9 % (272 of 296)	100.0 % (11 of 11)
Aromatic	91.3 % (115 of 126)	100.0 % (63 of 63)	81.4 % (48 of 59)	100.0 % (4 of 4)
Methyl	98.2 % (112 of 114)	96.5 % (55 of 57)	100.0 % (57 of 57)

1. ETS-1 Pointed domain

MECADVPLLT PSSKEMMSQA LKATFSGFTK EQQRLGIPKD PRQWTETHVR DWVMWAVNEF SLKGVDFQKF CMSGAALCAL GKECFLELAP DFVGDILWEH LEILQKEDVK

Show sample condition

Sample

Pressure 1.0 atm, Temperature 303 (±2) K

#	Name	Isotope labeling	Concentration
1	ETS-1 Pointed domain	[U-99% 15N; U-99% 13C]	1.2 ~ 0.0 mM
2	KPO4		10 mM
3	KCl		10 mM
4	DTT		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	null	direct	0.0
¹H	DSS	methyl protons	null	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	null	direct	0.0
¹H	DSS	methyl protons	null	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	null	direct	0.0
¹H	DSS	methyl protons	null	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	null	direct	0.0
¹H	DSS	methyl protons	null	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.58 ppm, Outliers: 2 Detail

Release date

1999-12-21

Citation

Structure of the Ets-1 Pointed Domain and Mitogen-activiated Protein Kinase Phosphorylation Site
Slupsky, C.M., Gentile, L.N., Donaldson, L.W., Mackereth, C.D., Seidel, J.J., Graves, B.J., Mcintosh, L.P.
Proc. Natl. Acad. Sci. U.S.A. (1998), 95, 12129-12134, PubMed , DOI:

Related entities 1. Ets-1 Pointed, : 1 : 2 : 4 : 1 : 23 entities Detail

Interaction partners 1. Ets-1 Pointed, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 17 experiments Detail

1 15N-HSQC