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BMRB: 4214


4214
2TMP
N-terminal Domain of Tissue Inhibitor of Metalloproteinase-2 (N-TIMP-2)
Authors
Muskett, F.W., Frenkiel, T.A., Feeney, J., Freedman, R.B., Carr, M.D., Williamson, R.A.
Assembly
Tissue Inhibitor of Metalloproteinases-2 (TIMP-2)
Entity
1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) (polymer, Thiol state: all disulfide bound), 127 monomers, 14090.00 Da Detail

CSCSPVHPQQ AFCNADVVIR TKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCE


Formula weight
14090.0 Da
Entity Connection
disulfide 3 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS1:SG1:CYS72:SG
2disulfidesing1:CYS3:SG1:CYS102:SG
3disulfidesing1:CYS13:SG1:CYS126:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING USING TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 82.0 %, Completeness (bb): 79.9 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All82.0 % (1206 of 1471)91.4 % (702 of 768)66.7 % (381 of 571)93.2 % (123 of 132)
Backbone79.9 % (599 of 750)95.3 % (246 of 258)64.7 % (240 of 371)93.4 % (113 of 121)
Sidechain85.6 % (717 of 838)89.4 % (456 of 510)79.2 % (251 of 317)90.9 % (10 of 11)
Aromatic29.8 % (31 of 104)46.2 % (24 of 52)11.8 % (6 of 51)100.0 % (1 of 1)
Methyl97.6 % (123 of 126)96.8 % (61 of 63)98.4 % (62 of 63)

1. tissue inhibitor of metalloproteinases-2 (TIMP-2)

CSCSPVHPQQ AFCNADVVIR TKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCE

Show sample condition
Sample

Pressure 1 atm, Temperature 308 (±0.1) K


#NameIsotope labelingTypeConcentration
1tissue inhibitor of metalloproteinases-2 (TIMP-2)[U-96% 13C; U-98% 15N]1.4 mM
2Sodium Phosphate25 mM
3potassium Chloride100 mM

Protein Blocks Logo
Calculated from 49 models in PDB: 2TMP, Strand ID: A Detail

Coupling constant
164 J values in 1 lists
Pressure 1 atm, Temperature 308 (±0.1) K Detail
Release date
2000-06-16
Citation 1
High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3
Muskett, F.W., Frenkiel, T.A., Feeney, J., Freedman, R.B., Carr, M.D., Williamson, R.A.
J. Biol. Chem. (1998), 273, 21736-21743, PubMed 9705310 , DOI: ,
Show more 2 citaions
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 ,
Citation 3
Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 ,
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    Title TIMP2_HUMAN Entity Metalloproteinase inhibitor 2
Related entities 1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2), : 1 : 6 : 63 entities Detail
More details for 70 related entities
Interaction partners 1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2), : 24 interactors Detail
More details for 24 interactors identified by 4 citations
Experiments performed 12 experiments Detail
nullKeywords Matrix Metalloproteinase Inhibitor, MMP Inhibitor, OB-fold, TIMP