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BMRB: 4242

3MSP

Motile Major Sperm Protein (MSP) of Ascaris suum

Authors

Haaf, A., LeClaire, L., Roberts, G., Kent, H.M., Roberts, T.M., Stewart, M., Neuhaus, D.

Assembly

Major Sperm Protein

Entity

1. Major Sperm Protein (polymer), 126 monomers, 14258.95 × 2 Da Detail

AQSVPPGDIN TQPSQKIVFN APYDDKHTYH IKITNAGGRR IGWAIKTTNM RRLSVDPPCG VLDPKEKVLM AVSCDTFNAA TEDLNNDRIT IEWTNTPDGA AKQFRREWFQ GDGMVRRKNL PIEYNL

Total weight

28517.9 Da

Max. entity weight

14258.95 Da

Source organism

Ascaris suum

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.8 %, Completeness (bb): 83.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.8 % (1249 of 1473)	89.8 % (693 of 772)	75.6 % (428 of 566)	94.8 % (128 of 135)
Backbone	83.6 % (617 of 738)	98.8 % (248 of 251)	68.4 % (253 of 370)	99.1 % (116 of 117)
Sidechain	87.0 % (742 of 853)	85.4 % (445 of 521)	90.8 % (285 of 314)	66.7 % (12 of 18)
Aromatic	97.2 % (105 of 108)	96.3 % (52 of 54)	98.0 % (50 of 51)	100.0 % (3 of 3)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. Major Sperm Protein

AQSVPPGDIN TQPSQKIVFN APYDDKHTYH IKITNAGGRR IGWAIKTTNM RRLSVDPPCG VLDPKEKVLM AVSCDTFNAA TEDLNNDRIT IEWTNTPDGA AKQFRREWFQ GDGMVRRKNL PIEYNL

Show sample condition

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.53 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.53 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 3MSP, Strand ID: A, B Detail

Release date

1999-01-24

Citation 1

Solution structure of the motile major sperm protein (MSP) of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation
Haaf, A., LeClaire, L., Roberts, G., Kent, H.M., Roberts, T.M., Stewart, M., Neuhaus, D.
J. Mol. Biol. (1998), 284, 1611-1624, PubMed 9878374 , DOI 10.1006/jmbi.1998.2291 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

Hide non-primary 1 citaion

Related entities 1. Major Sperm Protein, : 1 : 2 : 3 : 53 entities Detail

Interaction partners 1. Major Sperm Protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 NOESY

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

2 TOCSY

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

3 DQ-correlation

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

4 DQF-COSY

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

5 3D 15N HSQC-NOESY

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

6 3D 13C HSQC-NOESY

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

7 HCCH

Instrument

#1 Model Bruker AMX500 (500 MHz)

#2 Model Bruker DMX600 (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Concentration
1	Major Sperm Protein	2.5 ~ 3.5 mM
2	D2O	5 %
3	H2O	95 %

Sample #2

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Major Sperm Protein	[U-98%15N]	2.5 ~ 3.5 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 308 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Major Sperm Protein	[U-98%13C;U-98%15N]	2.5 ~ 3.5 mM
8	D2O		5 %
9	H2O		95 %

nullKeywords amoeboid motility, filaments, major sperm protein, NMR structure, polymerization

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Found 1 Document (1.854 seconds)

BMRB: 4242

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Major Sperm Protein, : 1 : 2 : 3 : 53 entities Detail

Interaction partners 1. Major Sperm Protein, : 1 interactors Detail

Experiments performed 7 experiments Detail

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