BMRB: 4243

Intercalated d(TCCCGTTTCCA) dimer

Authors

GALLEGO, J., CHOU, S., REID, B.R.

Assembly

DNA (5'-D(TCCCGTTTCCA)-3')

Entity

1. DNA (5'-D(TCCCGTTTCCA)-3') (polymer, Thiol state: not present), 11 monomers, 3323.107 × 2 Da Detail

TCCCGTTTCC A

Total weight

6646.214 Da

Max. entity weight

3323.107 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.4 %, Completeness (bb): 73.9 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	69.4 % (86 of 124)	67.3 % (76 of 113)	90.9 % (10 of 11)
Suger, PO4	73.9 % (65 of 88)	71.4 % (55 of 77)	90.9 % (10 of 11)
Nucleobase	58.3 % (21 of 36)	58.3 % (21 of 36)
Aromatic	77.3 % (17 of 22)	77.3 % (17 of 22)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. DNA 1

TCCCGTTTCC A

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Sample

Temperature 302 (±0.1) K, pH 4.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	DNA_1			3.9 mM

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Release date

2000-04-30

Citation 1

Centromeric pyrimidine strands fold into an intercalated motif by forming a double hairpin with a novel T:G:G:T tetrad: solution structure of the D(TCCCGTTTCCA) dimer
GALLEGO, J., CHOU, S., REID, B.R.
J. Mol. Biol. (1997), 273, 840-856, DOI 10.1006/jmbi.1997.1361

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Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Experiments performed 5 experiments Detail