BMRB: 4246

3D Structure of the M8L Mutant of Squash Trypsin Inhibitor CMTI-I, NMR, 6 Structures

Authors

Zhukov, I.YU., Bolewska, K., Bierzynski, A.

Assembly

CMTI-I(M8L)

Entity

1. CMTI-I(M8L) (polymer, Thiol state: not reported), 29 monomers, 3256.839 Da Detail

RVCPRILLEC KKDSDCLAEC VCLEHGYCG

Formula weight

3256.839 Da

Source organism

Cucurbita maxima

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.6 % (163 of 167)	97.6 % (163 of 167)
Backbone	93.2 % (55 of 59)	93.2 % (55 of 59)
Sidechain	100.0 % (108 of 108)	100.0 % (108 of 108)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (15 of 15)	100.0 % (15 of 15)

1. CMTI-I(M8L)

RVCPRILLEC KKDSDCLAEC VCLEHGYCG

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Sample

Pressure 1 atm, Temperature 303 (±0.2) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CMTI-I(M8L)			0.0 ~ 10.0 mM
2	NaCl			50 mM

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Calculated from 20 models in PDB: 2V1V, Strand ID: A Detail

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Release date

2000-05-01

Citation 1

Conservative mutation Met8 --> Leu affects the folding process and structural stability of squash trypsin inhibitor CMTI-I
Zhukov, I.YU., Bolewska, K., Bierzynski, A.
Protein Sci. (2000), 9, 273-279, PubMed 10716179 , DOI 10.1110/ps.9.2.273 , Abstract

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Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI 10.1007/bf00211777 , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 2 citaions

Related entities 1. CMTI-I(M8L), : 1 : 1 : 10 : 53 entities Detail

Interaction partners 1. CMTI-I(M8L), : 1 interactors Detail

Experiments performed 3 experiments Detail