BMRB: 4254

1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA

Authors

McIntosh, P.B., Taylor, I.A., Smerdon, S.J., Frenkiel, T.A., Lane, A.N.

Assembly

Mbp1

Entity

1. Mbp1 (polymer), 135 monomers, 15298.13 Da Detail

SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHH

Formula weight

15298.13 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing, energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 52.1 %, Completeness (bb): 75.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	52.1 % (839 of 1611)	52.6 % (441 of 839)	42.8 % (270 of 631)	90.8 % (128 of 141)
Backbone	75.4 % (602 of 798)	85.6 % (232 of 271)	63.8 % (254 of 398)	89.9 % (116 of 129)
Sidechain	37.5 % (353 of 941)	36.8 % (209 of 568)	36.6 % (132 of 361)	100.0 % (12 of 12)
Aromatic	3.6 % (6 of 168)	4.8 % (4 of 84)	0.0 % (0 of 82)	100.0 % (2 of 2)
Methyl	38.8 % (52 of 134)	52.2 % (35 of 67)	25.4 % (17 of 67)

1. MLU1-BOX binding protein

SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHH

Show sample condition

Sample #1

Temperature 288 (±1.0) K, pH 7.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	MLU1-BOX binding protein	[U-15N]		0.8 ~ 1.3 mM

Sample #2

Temperature 288 (±1.0) K, pH 7.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	MLU1-BOX binding protein	[U-15N;U-13C]		0.8 ~ 1.3 mM

Sample #3

Temperature 288 (±1.0) K, pH 7.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	MLU1-BOX binding protein			0.8 ~ 1.3 mM

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 19 models in PDB: 1L3G, Strand ID: A Detail

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Release date

1999-10-25

Citation 1

1H, 15N and 13C assignments of the DNA binding domain of transcription factor Mbp1 from S. cerevisiae in both its free and the DNA bound forms, and 1H assignments of the free DNA
McIntosh, P.B., Taylor, I.A., Smerdon, S.J., Frenkiel, T.A., Lane, A.N.
J. Biomol. NMR (1999), 13, 397-398, PubMed 10353201 , Abstract

Show more 2 citaions

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

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Related entities 1. Mbp1, : 1 : 12 entities Detail

Interaction partners 1. Mbp1, : 19 interactors Detail

Experiments performed 1 experiments Detail