Sequence Specific 1H, 13C and 15N Assignment of the Methyl Binding Domain of the Methyl-CpG-binding Protein MeCP2.
ASASPKQRRS IIRDRGPMYD DPTLPEGWTR KLKQRKSGRS AGKYDVYLIN PQGKAFRSKV ELIAYFEKVG DTSLDPNDFD FTVTGRGSGS GC
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.3 % (928 of 1063) | 95.6 % (537 of 562) | 73.3 % (300 of 409) | 98.9 % (91 of 92) |
Backbone | 83.9 % (453 of 540) | 98.4 % (185 of 188) | 68.4 % (182 of 266) | 100.0 % (86 of 86) |
Sidechain | 91.9 % (556 of 605) | 94.1 % (352 of 374) | 88.4 % (199 of 225) | 83.3 % (5 of 6) |
Aromatic | 75.0 % (63 of 84) | 100.0 % (42 of 42) | 48.8 % (20 of 41) | 100.0 % (1 of 1) |
Methyl | 95.8 % (69 of 72) | 94.4 % (34 of 36) | 97.2 % (35 of 36) |
1. Methyl binding domain of MeCP2
ASASPKQRRS IIRDRGPMYD DPTLPEGWTR KLKQRKSGRS AGKYDVYLIN PQGKAFRSKV ELIAYFEKVG DTSLDPNDFD FTVTGRGSGS GCTemperature 291 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Methyl binding domain of MeCP2 | [U-15N; U-13C] | 1.0 mM | |
2 | H2O | 90 % | ||
3 | D2O | 10 % |