BMRB: 4296

1H, 13C, and 15N Chemical Shift Assignments for E. coli Cold-shock Protein A (CspA)

Authors

Feng, W., Tejero, R., Zimmerman, D.E., Inouye, M., Montelione, G.T.

Assembly

Major cold shock protein from E. coli

Entity

1. Major cold shock protein from E. coli (polymer), 70 monomers, 7403.187 Da Detail

MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK GPAAGNVTSL

Formula weight

7403.187 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

CONSTRAINT-VIOLATION MINIMIZATION IN DIHEDRAL ANGLE SPACE

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 90.0 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (704 of 782)	96.3 % (387 of 402)	80.1 % (245 of 306)	97.3 % (72 of 74)
Backbone	97.1 % (404 of 416)	98.0 % (145 of 148)	96.5 % (193 of 200)	97.1 % (66 of 68)
Sidechain	84.0 % (358 of 426)	95.3 % (242 of 254)	66.3 % (110 of 166)	100.0 % (6 of 6)
Aromatic	51.2 % (43 of 84)	100.0 % (42 of 42)	0.0 % (0 of 41)	100.0 % (1 of 1)
Methyl	90.3 % (56 of 62)	100.0 % (31 of 31)	80.6 % (25 of 31)

1. Cold shock protein A

MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK GPAAGNVTSL

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Sample

Temperature 303 (±0.5) K, Details Samples were prepared in H2O containing 5% deuterium oxide for locking purposes. Solutions were buffered at pH* 6.0 +/- 0.1.

#	Name	Isotope labeling	Type	Concentration
1	Cold shock protein A	[U-98% 13C; U-90% 15N]		1.0 ~ 3.0 mM
2	KH2PO4			50 mM
3	NaEDTA			0.1 mM
4	NaN3			1 mM

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.66 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 16 models in PDB: 3MEF, Strand ID: A Detail

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Release date

1999-02-09

Citation 1

Solution NMR structure and backbone dynamics of the major cold-shock protein (CspA) from Escherichia coli: evidence for conformational dynamics in the single-stranded RNA-binding site
Feng, W., Tejero, R., Zimmerman, D.E., Inouye, M., Montelione, G.T.
Biochemistry (1998), 37, 10881-10896, PubMed 9692981 , DOI 10.1021/bi980269j , Abstract

Show more 2 citaions

Citation 2

Solution NMR structure of the major cold shock protein (CspA) from Escherichia coli: identification of a binding epitope for DNA
Newkirk, K., Feng, W., Jiang, W., Tejero, R., Emerson, S.D., Inouye, M., Montelione, G.T.
Proc. Natl. Acad. Sci. U. S. A. (1994), 91, 5114-5118, PubMed 7515185 , DOI: , Abstract

Citation 3

Automated analysis of protein NMR assignments using methods from artificial intelligence
Zimmerman, D.E., Kulikowski, C.A., Huang, Y., Feng, W., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R., Montelione, G.T.
J. Mol. Biol. (1997), 269, 592-610, PubMed 9217263 , DOI 10.1006/jmbi.1997.1052 , Abstract

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Related entities 1. Major cold shock protein from E. coli, : 1 : 2 : 3 : 203 entities Detail

Interaction partners 1. Major cold shock protein from E. coli, : 11 interactors Detail

Experiments performed 18 experiments Detail

Chemical shift validation 3 contents Detail