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Found 1 Document (1.306 seconds)

BMRB: 4308

1C8P

1H and 15N Chemical Shift Assignments for Domain 4 of the Common Beta-Chain of the IL-3, IL-5 and GM-CSF Receptors

Authors

Mulhern, T.D., Booker, G.W.

Assembly

cytokine receptor common beta chain domain 4

Entity

1. cytokine receptor common beta chain domain 4 (polymer), 102 monomers, 12105.34 Da Detail

MIQMAPPSLN VTKDGDSYSL RWETMKMRYE HIDHTFEIQY RKDTATWKDS KTETLQNAHS MALPALEPST RYWARVRVRT SRTGYNGIWS EWSEARSWDT ES

Formula weight

12105.34 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	97.7 % (724 of 741)	97.8 % (617 of 631)	97.3 % (107 of 110)
Backbone	96.3 % (290 of 301)	96.1 % (195 of 203)	96.9 % (95 of 98)
Sidechain	98.6 % (434 of 440)	98.6 % (422 of 428)	100.0 % (12 of 12)
Aromatic	100.0 % (73 of 73)	100.0 % (67 of 67)	100.0 % (6 of 6)
Methyl	100.0 % (42 of 42)	100.0 % (42 of 42)

1. beta c domain 4

MIQMAPPSLN VTKDGDSYSL RWETMKMRYE HIDHTFEIQY RKDTATWKDS KTETLQNAHS MALPALEPST RYWARVRVRT SRTGYNGIWS EWSEARSWDT ES

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Release date

2000-01-04

Citation

1H and 15N chemical shift assignments for domain 4 of the common beta-chain of the IL-3, IL-5 and GM-CSF receptors
Mulhern, T.D., Bagley, C.J., Gaunt, C., Lopez, A.F., Vadas, M.A., D'Andrea, R.J., Booker, G.W.
J. Biomol. NMR (1999), 14, 281-282, PubMed 10481277 , Abstract

Related entities 1. cytokine receptor common beta chain domain 4, : 1 : 7 : 64 entities Detail

Interaction partners 1. cytokine receptor common beta chain domain 4, : 11 interactors Detail

More details for 11 interactors identified by 7 citations

Experiments performed 7 experiments Detail

1 NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

2 TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

3 DQF-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

4 1H-15N HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

5 1H-15N HMQC-J

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

6 1H-15N NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

7 1H-15N TOCSY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
1	beta c domain 4	[U-99% 15N]	0.7 mM
2	phosphate		10 mM
3	D20		10 %
4	H2O		90 %

Sample #2

Temperature 293 (±0.1) K, pH 6.1 (±0.05)

#	Name	Isotope labeling	Concentration
5	beta c domain 4	[U-99% 15N]	0.7 mM
6	phosphate		10 mM
7	D2O		100 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4308_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4308_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shift_one	Warning	821		100.0 (chain: 1, length: 102)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shift_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 102, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 710
  - ¹⁵N chemical shifts: 111
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
69	SER	HG	6.463
70	THR	HG1	6.463
100	THR	HG1	6.463

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	631	615	97.5
¹⁵N chemical shifts	119	111	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	203	193	95.1
¹⁵N chemical shifts	98	93	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	428	422	98.6
¹⁵N chemical shifts	21	18	85.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	46	97.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	67	100.0
¹⁵N chemical shifts	6	6	100.0

Keywords Cytokine Receptor Module, Fn3 Domain, WSXWS motif

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Found 1 Document (1.306 seconds)

BMRB: 4308

Sample

Related entities 1. cytokine receptor common beta chain domain 4, : 1 : 7 : 64 entities Detail

Interaction partners 1. cytokine receptor common beta chain domain 4, : 11 interactors Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail