BMRB: 4324

Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein.

Authors

Karimi, A., Matsumura, M., Wright, P.E., Dyson, H.JANE.

Assembly

B domain of protein A

Entity

1. B domain of protein A (polymer), 58 monomers, 6598.172 Da Detail

ADNKFNKEQQ NAFYEILHLP NLNEEQRNGF IQSLKDDPSQ SANLLAEAKK LNDAQAPK

Formula weight

6598.172 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	97.3 % (429 of 441)	97.0 % (361 of 372)	98.6 % (68 of 69)
Backbone	98.2 % (166 of 169)	98.2 % (112 of 114)	98.2 % (54 of 55)
Sidechain	96.7 % (263 of 272)	96.5 % (249 of 258)	100.0 % (14 of 14)
Aromatic	90.5 % (19 of 21)	90.5 % (19 of 21)
Methyl	100.0 % (25 of 25)	100.0 % (25 of 25)

1. B domain of protein A

ADNKFNKEQQ NAFYEILHLP NLNEEQRNGF IQSLKDDPSQ SANLLAEAKK LNDAQAPK

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Sample

Temperature 303 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	B domain of protein A	[U-95% 15N]		1.0 ~ 4.0 mM

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Release date

1999-07-11

Citation 1

Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein
Karimi, A., Matsumura, M., Wright, P.E., Dyson, H.JANE.
J. Pept. Res.

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. B domain of protein A, : 2 : 6 : 3 : 102 : 64 entities Detail

Interaction partners 1. B domain of protein A, : 1 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail