BMRB: 4334

1H, 13C, 15N Resonance Assignments of ARID Domain of Dead-Ringer Protein.

Authors

Iwahara, J., Clubb, R.T.

Assembly

ARID domain of dead-ringer protein

Entity

1. ARID domain of dead-ringer protein (polymer), 139 monomers, 16157.34 Da Detail

GSGWSFEEQF KQVRQLYEIN DDPKRKEFLD DLFSFMQKRG TPINRLPIMA KSVLDLYELY NLVIARGGLV DVINKKLWQE IIKGLHLPSS ITSAAFTLRT QYMKYLYPYE CEKKNLSTPA ELQAAIDGNR REGRRSSYG

Formula weight

16157.34 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 92.6 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.6 % (1581 of 1707)	96.8 % (870 of 899)	85.9 % (567 of 660)	97.3 % (144 of 148)
Backbone	98.1 % (806 of 822)	98.2 % (276 of 281)	98.0 % (400 of 408)	97.7 % (130 of 133)
Sidechain	89.0 % (903 of 1015)	96.1 % (594 of 618)	77.2 % (295 of 382)	93.3 % (14 of 15)
Aromatic	81.6 % (124 of 152)	88.2 % (67 of 76)	74.3 % (55 of 74)	100.0 % (2 of 2)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. ARID domain of dead-ringer protein

GSGWSFEEQF KQVRQLYEIN DDPKRKEFLD DLFSFMQKRG TPINRLPIMA KSVLDLYELY NLVIARGGLV DVINKKLWQE IIKGLHLPSS ITSAAFTLRT QYMKYLYPYE CEKKNLSTPA ELQAAIDGNR REGRRSSYG

Show sample condition

Sample #1

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	ARID domain of dead-ringer protein	[U-15N]		1.5 mM
2	Tris-HCl	[U-2H]		20.0 mM
3	NaCl			100.0 mM
4	DTT	[U-2H]		2.0 mM
5	NaN3			0.01 %
6	ZnCl2			1.5 mM
7	H2O			92 %
8	D2O			8 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
9	ARID domain of dead-ringer protein	[U-13C; U-15N]		1.5 mM
10	Tris-HCl	[U-2H]		20.0 mM
11	NaCl			100.0 mM
12	DTT	[U-2H]		2.0 mM
13	NaN3			0.01 %
14	ZnCl2			1.5 mM
15	H2O			92 %
16	D2O			8 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1KQQ, Strand ID: A Detail

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Coupling constant

90 J values in 1 lists
Field strength (¹H) na MHz, Temperature 303 K, pH 6.7 Detail

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Temperature 303 K, pH 6.7

List #1 coupling_constant, Field strength (¹H) na MHz, Details 3D HNHA spectrum was used to measure 3JHNHA coupling constants. Error values for these couplings were estimated with the use of noise intensity of this spectrum.

Release date

1999-06-06

Citation

1H, 13C, 15N Resonance Assignments of ARID Domain of Dead-Ringer Protein
Iwahara, J., Clubb, R.T.

Related entities 1. ARID domain of dead-ringer protein, : 1 : 1 : 51 entities Detail

Interaction partners 1. ARID domain of dead-ringer protein, : 5 interactors Detail

Experiments performed 1 experiments Detail