BMRB: 4337

Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex.

Authors

Guilhaudis, L., Simorre, J., Bouchayer, E., Neuburger, M., Bourguignon, J., Douce, R., Marion, D., Gans, P.

Assembly

Oxidized form of the glycine cleavage system H-protein

Entity

1. glycine cleavage system H-protein (polymer), 131 monomers, 13947.34 Da Detail

SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H

2. LPA (non-polymer), 206.326 Da

Total weight

14153.666 Da

Max. entity weight

13947.34 Da

Entity Connection

amide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	amide	sing	1:LYS63:NZ	2:LPA1:CO

Source organism

Pisum sativum

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 33.0 %, Completeness (bb): 64.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	33.0 % (287 of 871)	22.5 % (167 of 741)	92.3 % (120 of 130)
Backbone	64.7 % (253 of 391)	49.6 % (133 of 268)	97.6 % (120 of 123)
Sidechain	7.1 % (34 of 480)	7.2 % (34 of 473)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 51)	0.0 % (0 of 49)	0.0 % (0 of 2)
Methyl	8.1 % (6 of 74)	8.1 % (6 of 74)

1. glycine cleavage system H-protein

SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H

Show sample condition

Sample

Temperature 291 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	glycine cleavage system H-protein	[U-99% 15N]		1.3 mM
2	potassium phosphate			50 mM
3	EDTA			0.1 mM
4	NaN3			0.2 %
5	D2O			10 %
6	H2O			90 %

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Release date

1999-05-09

Citation 1

Backbone and sequence-specific assignment of three forms of the lipoate-containing H-protein of the glycine decarboxylase complex
Guilhaudis, L., Simorre, J., Bouchayer, E., Neuburger, M., Bourguignon, J., Douce, R., Marion, D., Gans, P.
J. Biomol. NMR (1999), 15, 185-186, PubMed 10605092 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. glycine cleavage system H-protein, : 1 : 3 : 161 entities Detail

Interaction partners 1. glycine cleavage system H-protein, : 1 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail