BMRB: 4338

Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex.

Authors

Guilhaudis, L., Simorre, J., Bouchayer, E., Neuburger, M., Bourguignon, J., Douce, R., Marion, D., Gans, P.

Assembly

Glycine Cleavage system apoprotein H

Entity

1. Glycine Cleavage system apoprotein H (polymer), 131 monomers, 13947.34 Da Detail

SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H

Formula weight

13947.34 Da

Source organism

Pisum sativum

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 89.3 %, Completeness: 30.9 %, Completeness (bb): 63.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	30.9 % (269 of 871)	20.5 % (152 of 741)	90.0 % (117 of 130)
Backbone	63.2 % (247 of 391)	48.5 % (130 of 268)	95.1 % (117 of 123)
Sidechain	4.6 % (22 of 480)	4.7 % (22 of 473)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 51)	0.0 % (0 of 49)	0.0 % (0 of 2)
Methyl	9.5 % (7 of 74)	9.5 % (7 of 74)

1. Glycine Cleavage System apoprotein H

SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H

Show sample condition

Sample

Temperature 291 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Glycine Cleavage System apoprotein H	[U-99% 15N]		1.0 mM
2	potassium phosphate			50 mM
3	EDTA			0.1 mM
4	NaN3			0.2 %
5	D2O			10 %
6	H2O			90 %

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Chem. Shift Complete2

Sequence coverage: 9.2 %, Completeness: 18.2 %, Completeness (bb): 35.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	18.2 % (317 of 1742)	12.8 % (190 of 1482)	48.8 % (127 of 260)
Backbone	35.2 % (275 of 782)	27.6 % (148 of 536)	51.6 % (127 of 246)
Sidechain	4.4 % (42 of 960)	4.4 % (42 of 946)	0.0 % (0 of 14)
Aromatic	2.0 % (2 of 102)	2.0 % (2 of 98)	0.0 % (0 of 4)
Methyl	8.8 % (13 of 148)	8.8 % (13 of 148)

1. Glycine Cleavage System apoprotein H

SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H

Show sample condition

Sample

Temperature 291 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Glycine Cleavage System apoprotein H	[U-99% 15N]		1.0 mM
2	potassium phosphate			50 mM
3	EDTA			0.1 mM
4	NaN3			0.2 %
5	D2O			10 %
6	H2O			90 %

Hide sample codition

Release date

1999-05-09

Citation 1

Backbone and sequence-specific assignment of three forms of the lipoate-containing H-protein of the glycine decarboxylase complex
Guilhaudis, L., Simorre, J., Bouchayer, E., Neuburger, M., Bourguignon, J., Douce, R., Marion, D., Gans, P.
J. Biomol. NMR (1999), 15, 185-186, PubMed 10605092 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Glycine Cleavage system apoprotein H, : 1 : 3 : 161 entities Detail

Interaction partners 1. Glycine Cleavage system apoprotein H, : 1 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail