BMRB: 4351

A 30-residue Fragment of the Carp Granulin-1 Protein Folds into a Stack of two Beta-hairpins Similar to that found in the Native Protein

Authors

Vranken, W.F., Chen, Z.G., Xu, P., James, S., Bennett, H.P.J., Ni, F.

Assembly

granulin N-terminal peptide

Entity

1. granulin N-terminal peptide (polymer, Thiol state: not reported), 30 monomers, 3194.591 Da Detail

VIHCDAATIC PDGTTCSLSP YGVWYCSPFS

Formula weight

3194.591 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS16:SG
2	disulfide	sing	1:CYS10:SG	1:CYS26:SG

Source organism

Cyprinus carpio

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY AND SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 71.1 %, Completeness (bb): 73.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	71.1 % (204 of 287)	96.2 % (151 of 157)	40.8 % (53 of 130)
Backbone	73.5 % (108 of 147)	93.2 % (55 of 59)	60.2 % (53 of 88)
Sidechain	72.6 % (122 of 168)	98.0 % (96 of 98)	37.1 % (26 of 70)
Aromatic	51.2 % (21 of 41)	100.0 % (21 of 21)	0.0 % (0 of 20)
Methyl	56.7 % (17 of 30)	100.0 % (15 of 15)	13.3 % (2 of 15)

1. granulin

VIHCDAATIC PDGTTCSLSP YGVWYCSPFS

Show sample condition

Sample

Pressure 1 atm, Temperature 288.0 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	granulin			0.54 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1QGM, Strand ID: A Detail

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Coupling constant

5 J values in 1 lists
Pressure 1 atm, Temperature 288.0 (±0.2) K, pH 4.5 (±0.1) Detail

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Pressure 1 atm, Temperature 288.0 (±0.2) K, pH 4.5 (±0.1)

List #1 J_values

Release date

2000-12-14

Citation

A 30-residue fragment of the carp granulin-1 protein folds into a stack of two beta-hairpins similar to that found in the native protein
Vranken, W.F., Chen, Z.G., Xu, P., James, S., Bennett, H.P.J., Ni, F.
J. Pept. Res. (1999), 53, 590-597, PubMed 10424355 , DOI: , Abstract

Related entities 1. granulin N-terminal peptide, : 1 : 1 : 2 : 42 entities Detail

Experiments performed 5 experiments Detail