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Found 1 Document (4.867 seconds)

BMRB: 4381

1C07

Letter to the Editor:Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)

Authors

Enmon, J.L., de Beer, T., Overduin, M.

Assembly

Third EH domain of human Eps15

Entity

1. Third EH domain of human Eps15 (polymer), 104 monomers, 11695.51 Da Detail

KRKTWVVSPA EKAKYDEIFL KTDKDMDGFV SGLEVREIFL KTGLPSTLLA HIWSLCDTKD CGKLSKDQFA LAFHLISQKL IKGIDPPHVL TPEMIPPSDR ASLQ

Formula weight

11695.51 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 90.8 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.8 % (1136 of 1251)	90.7 % (592 of 653)	90.7 % (450 of 496)	92.2 % (94 of 102)
Backbone	91.1 % (556 of 610)	92.2 % (190 of 206)	90.2 % (277 of 307)	91.8 % (89 of 97)
Sidechain	90.8 % (672 of 740)	90.2 % (403 of 447)	91.7 % (264 of 288)	100.0 % (5 of 5)
Aromatic	90.4 % (85 of 94)	93.6 % (44 of 47)	86.7 % (39 of 45)	100.0 % (2 of 2)
Methyl	96.8 % (120 of 124)	96.8 % (60 of 62)	96.8 % (60 of 62)

1. third EH domain of Eps15

KRKTWVVSPA EKAKYDEIFL KTDKDMDGFV SGLEVREIFL KTGLPSTLLA HIWSLCDTKD CGKLSKDQFA LAFHLISQKL IKGIDPPHVL TPEMIPPSDR ASLQ

Show sample condition

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.48 ppm, Outliers: 2 Detail

Release date

2000-03-19

Citation 1

Letter to the Editor: Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)
Enmon, J.L., de Beer, T., Overduin, M.
J. Biomol. NMR (2000), 16, 81-82, PubMed , DOI:

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. Third EH domain of human Eps15, : 1 : 2 : 210 entities Detail

Interaction partners 1. Third EH domain of human Eps15, : 72 interactors Detail

More details for 72 interactors identified by 27 citations

Experiments performed 8 experiments Detail

1 CBCA(CO)NH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

2 HNCACB

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

3 HCC-TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

4 CCC-TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

5 HCCH_TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

6 3D-15N-NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

7 HNCO

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

8 2D CB corelation with HD and HE

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

Sample #1

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
2	d-tris		10 mM
3	KCl		100 mM
4	NaN3		2 mM
5	APMSF		0.010 mM
6	d-DTT		0.1 ~ 40.0 mM
7	D2O		10 %
8	H2O		90 %

Sample #2

Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
9	third EH domain of Eps15	[U-13C; U-15N]	1.0 mM
10	d-tris		10 mM
11	KCl		100 mM
12	NaN3		2 mM
13	APMSF		0.010 mM
14	d-DTT		0.1 ~ 40.0 mM
15	D2O		99.9 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4381_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4381_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1250		96.2 (chain: 1, length: 104)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 104, Sequence coverage: 96.2%
- Total number of assignments
  - ¹H chemical shifts: 713
  - ¹³C chemical shifts: 445
  - ¹⁵N chemical shifts: 92
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
68	GLN	CD	180.13
78	GLN	CD	179.97
104	GLN	CD	181.25

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	653	589	90.2
¹³C chemical shifts	496	442	89.1
¹⁵N chemical shifts	105	92	87.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	206	189	91.7
¹³C chemical shifts	208	183	88.0
¹⁵N chemical shifts	97	87	89.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	447	400	89.5
¹³C chemical shifts	288	259	89.9
¹⁵N chemical shifts	8	5	62.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	62	96.9
¹³C chemical shifts	64	62	96.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	44	93.6
¹³C chemical shifts	45	39	86.7
¹⁵N chemical shifts	2	2	100.0

Keywords calcium-binding, EH domain, Eps15, peptide bindin

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Found 1 Document (4.867 seconds)

BMRB: 4381

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Third EH domain of human Eps15, : 1 : 2 : 210 entities Detail

Interaction partners 1. Third EH domain of human Eps15, : 72 interactors Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail