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Found 1 Document (1.983 seconds)

BMRB: 4389

Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa

Authors

Kashimori, H., Yoshida, T., Kijima, H., Shimahara, H., Uchiyama, S., Ishino, T., Shuda, M., Nakano, H., Shibata, Y., Saihara, Y., Ohkubo, T., Yoshida, T., Kaji, A., Kobayashi, Y.

Assembly

Ribosome Recycling Factor

Entity

1. Ribosome Recycling Factor (polymer, Thiol state: not present), 185 monomers, 20485.16 Da Detail

MINEIKKEAQ ERMGKTLEAL GHAFAKIRTG RAHPSILDSV MVSYYGADTP LRQVANVTVE DSRTLALAVF DKSMIQAVEK AIMTSDLGLN PATAGTTIRV PMPALTEETR KGYTKQARAE AEQARVSVRN IRRDALAQLK DLQKEKEISE DEERRAGDDV QKLTDKFIGE IEKALEAKEA DLMAV

Formula weight

20485.16 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 47.7 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.7 % (1005 of 2109)	38.3 % (422 of 1101)	50.0 % (408 of 816)	91.1 % (175 of 192)
Backbone	83.2 % (915 of 1100)	93.6 % (350 of 374)	71.6 % (391 of 546)	96.7 % (174 of 180)
Sidechain	10.3 % (122 of 1185)	9.9 % (72 of 727)	11.0 % (49 of 446)	8.3 % (1 of 12)
Aromatic	6.5 % (4 of 62)	12.9 % (4 of 31)	0.0 % (0 of 31)
Methyl	10.5 % (24 of 228)	10.5 % (12 of 114)	10.5 % (12 of 114)

1. Ribosome Recycling Factor

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

Release date

2000-12-14

Citation 1

Backbone NMR assignment and secondary structure of ribosome recycling factor (RRF) from Pseudomonas aeruginosa
Kashimori, H., Yoshida, T., Kijima, H., Shimahara, H., Uchiyama, S., Ishino, T., Shuda, M., Nakano, H., Shibata, Y., Saihara, Y., Ohkubo, T., Yoshida, T., Kaji, A., Kobayashi, Y.
J. Biomol. NMR (1999), 15, 341-342, PubMed 10685343 , DOI: , Abstract

Show more 1 citaion

Citation 2

A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 , Abstract

Hide non-primary 1 citaion

Related entities 1. Ribosome Recycling Factor, : 1 : 1 : 159 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

2 3D HNCA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

3 3D HN(CO)CA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

4 3D HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

6 3D HA(CA)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

7 3D HA(CACO)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

8 4D 1H-15N-15N-1H NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ribosome Recycling Factor	[U-99% 2H; U-99% 15N]		1.5 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ribosome Recycling Factor	[U-99% 2H; U-99% 13C; U-99% 15N]		1.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4389_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4389_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_set_1	OK	876		98.9 (chain: 1, length: 185)
1	Chemical shift references	chemical_shift_references_set_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_set_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 185, Sequence coverage: 98.9%
- Total number of assignments
  - ¹³C chemical shifts: 357
  - ¹H chemical shifts: 346
  - ¹⁵N chemical shifts: 173
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	816	357	43.8
¹H chemical shifts	1101	346	31.4
¹⁵N chemical shifts	206	173	84.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	370	357	96.5
¹H chemical shifts	374	346	92.5
¹⁵N chemical shifts	180	173	96.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	446	0	0.0
¹H chemical shifts	727	0	0.0
¹⁵N chemical shifts	26	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	121	0	0.0
¹H chemical shifts	121	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	31	0	0.0
¹H chemical shifts	31	0	0.0

Keywords backbone assignment, ribosome recycling factor, RRF, secondary structure

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Found 1 Document (1.983 seconds)

BMRB: 4389

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Related entities 1. Ribosome Recycling Factor, : 1 : 1 : 159 entities Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail