Search

Query history

Search help 
Download

Found 1 Document (3.211 seconds)

BMRB: 4419


4419
1CB9
NMR structure with tightly bound water molecules of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution (major form)
Authors
Dementieva, D.V., Bocharov, E.V., Arseniev, A.S.
Assembly
cytotoxin 2, major form
Entity
1. cytotoxin 2, major form (polymer), 60 monomers, 6636.139 Da Detail

LKCKKLVPLF SKTCPAGKNL CYKMFMVAAP HVPVKRGCID VCPKSSLLVK YVCCNTDKCN


Formula weight
6636.139 Da
Entity Connection
disulfide 4 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS3:SG1:CYS21:SG
2disulfidesing1:CYS14:SG1:CYS38:SG
3disulfidesing1:CYS42:SG1:CYS53:SG
4disulfidesing1:CYS54:SG1:CYS59:SG

Source organism
Naja oxiana
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING BY MOLECULAR DYNAMICS IN TORSION ANGLE SPACE
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 98.7 %, Completeness (bb): 97.4 % Detail
Download

Polymer type: polypeptide(L)

Total1H
All98.7 % (370 of 375)98.7 % (370 of 375)
Backbone97.4 % (114 of 117)97.4 % (114 of 117)
Sidechain99.2 % (256 of 258)99.2 % (256 of 258)
Aromatic100.0 % (20 of 20)100.0 % (20 of 20)
Methyl100.0 % (33 of 33)100.0 % (33 of 33)

1. cytotoxin 2

LKCKKLVPLF SKTCPAGKNL CYKMFMVAAP HVPVKRGCID VCPKSSLLVK YVCCNTDKCN

Show sample condition
Sample

Temperature 303 (±0.5) K, pH 5.5 (±0.1)


#NameIsotope labelingTypeConcentration
1cytotoxin 25.0 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1CB9, Strand ID: A Detail

Coupling constant
52 J values in 1 lists
Field strength (1H) 600 MHz, Temperature 303 (±0.5) K, pH 5.5 (±0.1) Detail
Release date
1999-11-11
Citation 1
Two forms of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution: spatial structures with tightly bound water molecules
Dementieva, D.V., Bocharov, E.V., Arseniev, A.S.
Eur. J. Biochem. (1999), 263, 152-162, PubMed 10429199 , DOI: ,
Show more 3 citaions
Citation 2
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 ,
Citation 3
Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 ,
Citation 4
MOLMOL: a program for display and analysis of macromolecular structures
Koradi, R., Billeter, M., Wuthrich, K.
J. Mol. Graph. (1996), 14, 51-5, 29, PubMed 8744573 , DOI: ,
Entries sharing articles BMRB: 52, Swiss-Prot: 1 entries Detail
  BMRB: 4503 released on 2008-07-16
    Title Solution Structure of Alpha-conotoxin SI
  BMRB: 4604 released on 2008-07-16
    Title NMR STRUCTURE OF THE PEPTAIBOL CHRYSOSPERMIN C BOUND TO DPC MICELLES
  BMRB: 5548 released on 2008-07-16
    Title Bovine Pancreatic Polypeptide (bPP) undergoes significant changes in conformation and dynamics upon binding to DPC micelles
  BMRB: 4921 released on 2008-07-15
    Title Solution structure of poneratoxin
  BMRB: 4396 released on 2007-06-18
    Title Anticoagulant protein from the nematode Ancylostoma caninum
  BMRB: 7080 released on 2006-10-30
    Title Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region")of tropomyosin
  BMRB: 7106 released on 2006-08-14
    Title NMR Assignment of human RGS18 (regulator of G-protein signalling)
  BMRB: 6723 released on 2005-10-13
    Title NMR structure of the heme chaperone CcmE reveals a novel functional motif
  BMRB: 6313 released on 2005-02-07
    Title 1H,13C,and 15N NMR assignments of the Bombyx mori Pheromone-binding Protein fragment BmPBP(1-128) at pH 6.5.
  BMRB: 5844 released on 2004-07-25
    Title ZR18 Structure determination
  BMRB: 6176 released on 2004-06-24
    Title Solution structure of a ubiquitin-like domain of tubulin-folding cofactor B
  BMRB: 6056 released on 2004-05-14
    Title Yeast oligosaccharyltransferase subunit Ost4p
  BMRB: 5859 released on 2003-10-16
    Title Structure of Antibacterial Peptide Microcin J25: A 21-Residue Lariat Protoknot
  BMRB: 5792 released on 2003-10-15
    Title Assignment of the 1H, 15N and 13C resonances of E. coli frataxin orthologue, CyaY
  BMRB: 5596 released on 2003-09-04
    Title Solution Structure of the hypothetical protein yggU from E. coli. Northeast Structural Genomics Consortium Target ER14.
  BMRB: 5517 released on 2003-06-11
    Title NMR studies of the DNA-binding domain of B-Myb
  BMRB: 4982 released on 2003-05-22
    Title 1H, 13C and 15N NMR sequence-specific resonance assignments for bovine apo-S100A1(aa) in oxidized form
  BMRB: 4943 released on 2003-05-20
    Title 1H Chemical shift Assignments for Lysozymes marketed by Seikagaku and Sigma companies at pH 3.8, 308K
  BMRB: 5690 released on 2003-05-07
    Title 1H, 13C, and 15N Chemical Shift Assignments for Cobra Neurotoxin II
  BMRB: 5670 released on 2003-04-29
    Title Structural Characterization of Hellethionins from helleborus purpurascens
  BMRB: 5691 released on 2003-02-24
    Title Solution Structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19
  BMRB: 5379 released on 2003-02-19
    Title Bovine Pancreatic Polypeptide Bound to DPC Micelles
  BMRB: 5184 released on 2002-05-07
    Title 1H Chemical Shift Assignments for toxin BeKm from the Scorpion Buthus eupeus
  BMRB: 5010 released on 2002-04-04
    Title 1H, 15N, and 13C chemical shift assignments for DNA polymerase X
  BMRB: 4792 released on 2002-04-03
    Title Backbone NMR Assignment and Secondary Structure of the Dimeric ParD Protein
  BMRB: 5275 released on 2002-03-21
    Title The rhesus rotavirus sialic acid binding domain without ligand
  BMRB: 5044 released on 2001-11-13
    Title 1H, 13C, and 15N Chemical shift assignments for the third Immunoglobulin domain from the neural cell adhesion molecule, N-CAM
  BMRB: 4979 released on 2001-11-08
    Title 1H and 13C chemical shift assignments for the pheromone Er-23 from Euplotes raikovi
  BMRB: 4964 released on 2001-08-07
    Title 1H, 13C and 15N resonance assignment for barnase
  BMRB: 4936 released on 2001-07-30
    Title 1H, 13C and 15N chemical shift assignments of the outer membrane protein OmpX from E.coli in DHPC micelles
  BMRB: 4935 released on 2001-06-28
    Title Backbone Assignments for Af19
  BMRB: 4953 released on 2001-06-28
    Title Assignment of 1H, 13C and 15N resonances of the PPIase domain of the trigger factor from Mycoplasma genitalium
  BMRB: 4774 released on 2001-05-02
    Title Three-dimensional Structure of the Histidine-containing Phosphocarrier Protein (Hpr) from Enterococcus faecalis in Solution
  BMRB: 4584 released on 2001-05-01
    Title Solution Structure of the DNA-binding Domain of TraM
  BMRB: 4901 released on 2001-04-25
    Title 1H, 15N, 13C assignments of the N-terminal domain of the human TFIIH p62 subunit
  BMRB: 4980 released on 2001-03-24
    Title 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease
  BMRB: 4820 released on 2001-01-28
    Title 1H and 13Ca Chemical Shift Assignments for the Pheromone Er-22
  BMRB: 4422 released on 2000-12-18
    Title NMR Solution Structure of Apis mellifera Chymotrypsin Inhibitor (AMCI).
  BMRB: 4373 released on 2000-12-13
    Title 1H, 15N, and 13C Resonance Assignment of the PH Domain from C. elegans UNC-89
  BMRB: 4602 released on 2000-12-05
    Title Solution Structure of Oxidized Microsomal Rabbit Cytochrome b5. Factors Determining the Heterogeneous Binding of the Heme
  BMRB: 4890 released on 2000-12-03
    Title 1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit
  BMRB: 4815 released on 2000-11-12
    Title NMR structure of Cardiotoxin in DPC-micelle
  BMRB: 4215 released on 2000-10-01
    Title Staphylokinase (NMR, Sakstar Variant)
  BMRB: 4214 released on 2000-06-16
    Title N-terminal Domain of Tissue Inhibitor of Metalloproteinase-2 (N-TIMP-2)
  BMRB: 4246 released on 2000-05-01
    Title 3D Structure of the M8L Mutant of Squash Trypsin Inhibitor CMTI-I, NMR, 6 Structures
  BMRB: 4438 released on 2000-03-22
    Title 1H, 13C and 15N chemical Shift Assignments for SCP2
  BMRB: 4411 released on 2000-03-16
    Title Backbone 1H and 15N Chemical Shift Assignments for Transforming Growth Factor beta
  BMRB: 4429 released on 1999-12-22
    Title 1H and 15N Chemical Shift Assignments for ribosomal protein L7
  BMRB: 4418 released on 1999-11-23
    Title NMR structure with tightly bound water molecules of cytotoxin II (cardiotoxin) from Naja naja oxiana in aqueous solution (minor form)
  BMRB: 4256 released on 1999-11-22
    Title 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA
  BMRB: 4254 released on 1999-10-25
    Title 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA
  BMRB: 4293 released on 1999-03-08
    Title Assignment of 1H, 13C and 15N Signals of the DNase Domain of Colicin E9
  Swiss-Prot: P01441 released on 1986-07-21
    Title CX2_NAJOX Entity Cytotoxin 2
Related entities 1. cytotoxin 2, major form, : 1 : 3 : 1 : 2 : 192 entities Detail
More details for 199 related entities
Experiments performed 4 experiments Detail
nullKeywords bound water, cardiotoxin, cis/trans isomerization, cytotoxin II, membrane perturbation