BMRB: 4429

1H and 15N Chemical Shift Assignments for ribosomal protein L7

Authors

Bocharov, E.V.

Assembly

ribosomal protein L7 from E. coli

Entity

1. ribosomal protein L7 from E. coli (polymer, Thiol state: not present), 120 monomers, 12163.84 × 2 Da Detail

SITKDQIIEA VAAMSVMDVV ELISAMEEKF GVSAAAAVAV AAGPVEAAEE KTEFDVILKA AGANKVAVIK AVRGATGLGL KEAKDLVESA PAALKEGVSK DDAEALKKAL EEAGAEVEVK

Total weight

24327.68 Da

Max. entity weight

12163.84 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing combined with molecular dynamics in torsion angle space

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.4 %, Completeness (bb): 97.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	95.4 % (733 of 768)	95.1 % (616 of 648)	97.5 % (117 of 120)
Backbone	97.5 % (355 of 364)	97.6 % (240 of 246)	97.5 % (115 of 118)
Sidechain	93.6 % (378 of 404)	93.5 % (376 of 402)	100.0 % (2 of 2)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	98.9 % (90 of 91)	98.9 % (90 of 91)

1. E.coli ribosomal protein L7/L12

SITKDQIIEA VAAMSVMDVV ELISAMEEKF GVSAAAAVAV AAGPVEAAEE KTEFDVILKA AGANKVAVIK AVRGATGLGL KEAKDLVESA PAALKEGVSK DDAEALKKAL EEAGAEVEVK

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.9 (±0.1), Details The protein L7/L12 degraded during 2-3 weeks due to spontaneous proteolysis in the hinge region.

#	Name	Isotope labeling	Type	Concentration
1	E.coli ribosomal protein L7/L12	[U-95% 15N]		1.0 mM

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 1RQU, Strand ID: A, B Detail

---

Coupling constant

118 J values in 1 lists
Temperature 303 (±0.5) K, pH 6.9 (±0.1) Detail

---

Temperature 303 (±0.5) K, pH 6.9 (±0.1)

List #1 coupling_constants_set_1, Details The HN-HA J couplings of L7 dimer were measured using the HMQC experiment.

Release date

1999-12-22

Citation 1

Topology of the secondary structure elements of ribosomal protein L7/L12 from E.coli in solutio
Bocharov, E.V., Gudkov, A.T., Arseniev, A.S.
FEBS Lett. (1996), 379, 291-294, PubMed , DOI:

Show more 2 citaions

Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Citation 3

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Hide non-primary 2 citaions

Related entities 1. ribosomal protein L7 from E. coli, : 1 : 28 : 3 : 3 : 134 entities Detail

Interaction partners 1. ribosomal protein L7 from E. coli, : 167 interactors Detail

Experiments performed 6 experiments Detail