BMRBj - BMREntryMaster

Found 1 Document (1.294 seconds)

BMRB: 4432

4X5T

1H-NMR Spectral Assignment of Second Transmembrane Segments of Human Glycine Receptor

Authors

Tang, P., Zubrzycki, I.Z., Hu, J., Xu, Y.

Assembly

glycine receptor alpha 1 subunit TM2 segment

Entity

1. glycine receptor alpha 1 subunit TM2 segment (polymer, Thiol state: not present), 23 monomers, 2304.623 Da Detail

PARVGLGITT VLTMTTQSSG SRA

Formula weight

2304.623 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 83.9 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	83.9 % (99 of 118)	83.9 % (99 of 118)
Backbone	95.8 % (46 of 48)	95.8 % (46 of 48)
Sidechain	75.7 % (53 of 70)	75.7 % (53 of 70)
Methyl	94.1 % (16 of 17)	94.1 % (16 of 17)

1. Neurotransmitter-gated receptor

PARVGLGITT VLTMTTQSSG SRA

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 4.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Neurotransmitter-gated receptor			3.1 mM
4	DPC			0.0 ~ 0.0 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 83.9 %, Completeness (bb): 94.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	83.9 % (198 of 236)	83.9 % (198 of 236)
Backbone	94.8 % (91 of 96)	94.8 % (91 of 96)
Sidechain	76.4 % (107 of 140)	76.4 % (107 of 140)
Methyl	94.1 % (32 of 34)	94.1 % (32 of 34)

1. Neurotransmitter-gated receptor

PARVGLGITT VLTMTTQSSG SRA

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 4.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Neurotransmitter-gated receptor			3.1 mM
2	SDS			677 mM

Release date

2000-05-04

Citation

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Related entities 1. glycine receptor alpha 1 subunit TM2 segment, : 1 : 20 : 3 : 19 : 128 entities Detail

Interaction partners 1. glycine receptor alpha 1 subunit TM2 segment, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4432_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4432_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	OK	131	100.0 (chain: 1, length: 23)
2	Assigned chemical shifts	assigned_chemical_shifts_two	OK	131	100.0 (chain: 1, length: 23)
1	Chemical shift references	chemical_shift_reference	OK	1	No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 131
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: assigned_chemical_shifts_two
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 131
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.294 seconds)

BMRB: 4432

Sample

Sample

Related entities 1. glycine receptor alpha 1 subunit TM2 segment, : 1 : 20 : 3 : 19 : 128 entities Detail

Interaction partners 1. glycine receptor alpha 1 subunit TM2 segment, : 2 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail