BMRBj - BMREntryMaster

Found 1 Document (1.26 seconds)

BMRB: 4433

3JAD

1H-NMR Spectral Assignment of Second Transmembrane Segments of Human Glycine Receptor

Authors

Tang, P., Zubrzycki, I.Z., Hu, J., Xu, Y.

Assembly

glycine receptor alpha 1 subunit TM2 segment S267Y

Entity

1. glycine receptor alpha 1 subunit TM2 segment S267Y (polymer, Thiol state: not present), 23 monomers, 2380.719 Da Detail

PARVGLGITT VLTMTTQYSG SRA

Formula weight

2380.719 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 89.3 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	89.3 % (109 of 122)	89.3 % (109 of 122)
Backbone	95.8 % (46 of 48)	95.8 % (46 of 48)
Sidechain	85.1 % (63 of 74)	85.1 % (63 of 74)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. Neurotransmitter-gated receptor

PARVGLGITT VLTMTTQYSG SRA

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 4.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Neurotransmitter-gated receptor			3.1 mM
2	SDS			677 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	90.2 % (220 of 244)	90.2 % (220 of 244)
Backbone	95.8 % (92 of 96)	95.8 % (92 of 96)
Sidechain	86.5 % (128 of 148)	86.5 % (128 of 148)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (34 of 34)	100.0 % (34 of 34)

1. Neurotransmitter-gated receptor

PARVGLGITT VLTMTTQYSG SRA

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 4.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Neurotransmitter-gated receptor			3.1 mM
4	DPC			0.0 ~ 0.0 mM

Release date

2000-05-04

Citation 1

NMR structures of the second transmembrane domain of the human glycine receptor alpha(1) subunit: model of pore architecture and channel gating
Tang, P., Mandal, P.K., Xu, Y.
Biophys. J. (2002), 83, 252-262, PubMed 12080117 , DOI 10.1016/S0006-3495(02)75166-7 , Abstract

Show more 2 citaions

Citation 2

Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes
Grenningloh, G., Schmieden, V., Schofield, P.R., Seeburg, P.H., Siddique, T., Mohandas, T.K., Becker, C.M., Betz, H.
EMBO J. (1990), 9, 771-776, PubMed 2155780 , DOI: , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 2 citaions

Related entities 1. glycine receptor alpha 1 subunit TM2 segment S267Y, : 1 : 39 : 88 entities Detail

Interaction partners 1. glycine receptor alpha 1 subunit TM2 segment S267Y, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4433_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4433_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_one	OK	142	100.0 (chain: 1, length: 23)
2	Assigned chemical shifts	assigned_chemical_shifts_two	OK	143	100.0 (chain: 1, length: 23)
1	Chemical shift references	chemical_shift_reference	OK	1	No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_one
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 142
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: assigned_chemical_shifts_two
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 143
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	122	108	88.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	46	95.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	62	83.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	17	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	122	109	89.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	46	95.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	63	85.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	17	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0

Keywords general anesthetics, glycine receptor, transmembrane domain 2 (TM2)

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Found 1 Document (1.26 seconds)

BMRB: 4433

Sample

Sample

Related entities 1. glycine receptor alpha 1 subunit TM2 segment S267Y, : 1 : 39 : 88 entities Detail

Interaction partners 1. glycine receptor alpha 1 subunit TM2 segment S267Y, : 2 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail