BMRB: 4584

Solution Structure of the DNA-binding Domain of TraM

Authors

Stockner, T., Plugariu, C., Koraimann, G., Hoegenauer, G., Bermel, W., Prytulla, S., Sterk, H.

Assembly

DNA BINDING DOMAIN OF THE TRAM PROTEIN

Entity

1. DNA BINDING DOMAIN OF THE TRAM PROTEIN (polymer, Thiol state: not present), 55 monomers, 6280.147 Da Detail

AKVQAYVSDE IVYKINKIVE RRRAEGAKST DVSFSSISTM LLELGLRVYE AQMER

Formula weight

6280.147 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry; simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 98.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	97.2 % (384 of 395)	97.0 % (327 of 337)	98.3 % (57 of 58)
Backbone	98.8 % (165 of 167)	99.1 % (111 of 112)	98.2 % (54 of 55)
Sidechain	96.1 % (219 of 228)	96.0 % (216 of 225)	100.0 % (3 of 3)
Aromatic	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (35 of 35)	100.0 % (35 of 35)

1. R1 PLASMID TRAM PROTEIN

AKVQAYVSDE IVYKINKIVE RRRAEGAKST DVSFSSISTM LLELGLRVYE AQMER

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Sample #1

Pressure 1 atm, Temperature 300 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	R1 PLASMID TRAM PROTEIN			4 mg/mL
2	phosphate buffer			50 mM
3	H2O			95 %
4	D2O			5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
5	R1 PLASMID TRAM PROTEIN	[U-15N]		1.3 mg/mL
6	phosphate buffer			50 mM
7	H2O			95 %
8	D2O			5 %

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Calculated from 20 models in PDB: 1DP3, Strand ID: A Detail

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Release date

2001-05-01

Citation 1

Solution structure of the DNA-binding domain of TraM
Stockner, T., Plugariu, C., Koraimann, G., Hoegenauer, G., Bermel, W., Prytulla, S., Sterk, H.
Biochemistry (2001), 40, 3370-3377, PubMed 11258958 , DOI: , Abstract

Show more 4 citaions

Citation 2

X-PLOR, version 3.1: A system for X-ray crystallography and NMR
Brunger, A.T.

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 4

MOLMOL: a program for display and analysis of macromolecular structures
Koradi, R., Billeter, M., Wuthrich, K.
J. Mol. Graph. (1996), 14, 51-5, 29, PubMed 8744573 , DOI: , Abstract

Citation 5

Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy
Kraulis, P.J., Domaille, P.J., Campbell-Burk, S.L., Van Aken, T., Laue, E.D.
Biochemistry (1994), 33, 3515-3531, PubMed 8142349 , Abstract

Hide non-primary 4 citaions

Related entities 1. DNA BINDING DOMAIN OF THE TRAM PROTEIN, : 1 : 1 : 12 entities Detail

Experiments performed 3 experiments Detail